Effects of reciprocal full-sib selection in maize

1985 ◽  
Author(s):  
Louis Robert Reeder
Keyword(s):  
1985 ◽  
Vol 5 (9) ◽  
pp. 2272-2278
Author(s):  
R A Akins ◽  
A M Lambowitz

We have developed a sib selection procedure for cloning Neurospora crassa nuclear genes by complementation of mutants. This procedure takes advantage of a modified N. crassa transformation procedure that gives as many as 10,000 to 50,000 stable transformants per microgram of DNA with recombinant plasmids containing the N. crassa qa-2+ gene. Here, we describe the use of the sib selection procedure to clone genes corresponding to auxotrophic mutants, nic-1 and inl. The identities of the putative clones were confirmed by mapping their chromosomal locations in standard genetic crosses and using restriction site polymorphisms as genetic markers. Because we can obtain very high N. crassa transformation frequencies, cloning can be accomplished with as few as five subdivisions of an N. crassa genomic library. The sib selection procedure should, for the first time, permit the cloning of any gene corresponding to an N. crassa mutant for which an appropriate selection can be devised. Analogous procedures may be applicable to other filamentous fungi before the development of operational shuttle vectors.


Crop Science ◽  
1986 ◽  
Vol 26 (2) ◽  
pp. 275-278 ◽  
Author(s):  
Malkit Singh ◽  
A. S. Khehra ◽  
B. S. Dhillon

1987 ◽  
pp. 445-449 ◽  
Author(s):  
Mary McCormick
Keyword(s):  

1990 ◽  
Vol 56 (1) ◽  
pp. 49-56 ◽  
Author(s):  
Andrew G. Clark ◽  
Frances M. Szumski ◽  
Karen A. Bell ◽  
Lisa Esterling Keith ◽  
Sharyn Houtz ◽  
...  

SummaryA large outbred population ofDrosophila melanogasterwas subjected to artificial selection on lipid and glycogen storage. In three separate experiments, two replicates underwent sib selection for both increased and decreased storage. In the first study, flies were selected on the basis of total triacylglycerol for ten generations. This experiment resulted in no significant direct response, but there was a significant change in total body weight, underscoring the importance of concern for the allometric relationship between body weight and lipid content. In the second study, selection was performed for 15 generations on the percentage of body composition that was triacylglycerol. A significant direct response was obtained, and the two replicates revealed heritability estimates of 0·40 and 0·43. The third study selected glycogen content for 15 generations, and produced a significant response with heritabilities of 0·25 and 0·31. A series of 12 biochemical and enzyme kinetic traits was examined at five generation intervals in all experiments, and a number of correlated responses were detected. The results are interpreted with respect to the evolutionary constraints on energy storage evolution and the genetic basis of the allometric relationship between body weight and fat content.


2007 ◽  
Vol 42 (10) ◽  
pp. 1383-1391 ◽  
Author(s):  
José Marcelo Soriano Viana

The objectives of this work were to analyze theoretical genetic gains of maize due to recurrent selection among full-sib and half-sib families, obtained by Design I, Full-Sib Design and Half-Sib Design, and genotypic variability and gene loss with long term selection. The designs were evaluated by simulation, based on average estimated gains after ten selection cycles. The simulation process was based on seven gene systems with ten genes (with distinct degrees of dominance), three population classes (with different gene frequencies), under three environmental conditions (heritability values), and four selection strategies. Each combination was repeated ten times, amounting to 25, 200 simulations. Full-sib selection is generally more efficient than half-sib selection, mainly with favorable dominant genes. The use of full-sib families derived by Design I is generally more efficient than using progenies obtained by Full-Sib Design. Using Design I with 50 males and 200 females (effective size of 160) did not result in improved populations with minimum genotypic variability. In the populations with lower effective size (160 and 400) the loss of favorable genes was restricted to recessive genes with reduced frequencies.


Crop Science ◽  
2009 ◽  
Vol 49 (2) ◽  
pp. 443-450 ◽  
Author(s):  
Brandon M. Wardyn ◽  
Jode W. Edwards ◽  
Kendall R. Lamkey

2007 ◽  
Vol 115 (2) ◽  
pp. 289-297 ◽  
Author(s):  
K. C. Falke ◽  
H. P. Maurer ◽  
A. E. Melchinger ◽  
H.- P. Piepho ◽  
C. Flachenecker ◽  
...  

1991 ◽  
Vol 69 (10) ◽  
pp. 2130-2134 ◽  
Author(s):  
Chih-Cheng T. Chao ◽  
Albert H. Ellingboe

Sib selection for mating competence among isolates of Magnaporthe grisea that are pathogenic on rice has led to the selection of isolates where the mating type alleles are the only restriction on mating competence. Isolates are able to mate with all sibling isolates of opposite mating type and produce asci with ascospores. Isolates can function as male, female, or hermaphrodite in mating depending on the isolates with which they are mated. Mating competence is controlled by the genes in both mated isolates. Key words: mating competence, sib selection, rice blast, genetics.


1987 ◽  
Vol 73 (5) ◽  
pp. 672-674 ◽  
Author(s):  
B. S. Dhillon ◽  
A. S. Khehra ◽  
M. Singh

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