scholarly journals Isolasi dan Identifikasi Bakteri Endofit Pada Akar Cabai (Capsicum annuum L.) Untuk Menghambat Pertumbuhan Jamur (Fusarium oxysporum)

2020 ◽  
Vol 2 (2) ◽  
pp. 93-98
Author(s):  
Shela Fahdila ◽  
Ferdinand Susilo ◽  
Abdul Karim

Penelitian ini bertujuan untuk mendapatkan isolat bakteri endofit dari akar cabai merah Capsicum annuum dan mengetahui kemampuannya dalam menghambat pertumbuhan jamur patogen Fusarium oxysporum. Penelitian ini menggunakan metode deskriptif berskala laboratorium. Hasil isolasi dari akar cabai merah Capsicum annuum ditemukan dua koloni isolat yang berbeda yang diberi dengan kode isolat A1 dan A2. Kedua isolat bakteri endofit diduga merupakan genus Bacillus. Hasil daya antagonis kedua isolat menyatakan bahwa bakteri endofit tersebut mampu menghambat pertumbuhan jamur patogen Fusarium oxysporum, hal ini dapat dilihat dari diameter zona hambat yang terbentuk.

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Temesgen Oljira ◽  
Sefawdin Berta

The yield of pepper (Capsicum annuum L.) is extremely threatened by different diseases in Ethiopia. The objective of the study was isolation of wilt-causing pathogens and susceptibility test of local growing pepper. Eighteen pepper farming fields were selected for disease assessment study. The samples of Mareko Fana, Dubi, and Mitmita local cultivar pepper’s pods, seeds, leaves, stems, and roots were collected, surface sterilized, and cultured on potato dextrose agar (PDA). Selective peptone pentachloronitrobenzene (PCNB) agar medium was used for fungus. Similarly, for bacteria isolation, nutrient agar (NA) was used. Morphological and biochemical tests revealed eleven fungal isolates of Fusarium oxysporum f. sp. that were isolated. The pathogenicity test confirmed nine of them were virulent to Mareko Fana, Dubi, and Mitmita local pepper. It is confirmed that Fusarium oxysporum f. sp. is the pathogen Fusarium oxysporum f. sp. capsici. Besides, Ralstonia solanacearum was identified as a bacterium pathogen causing complex pepper wilt disease. The highest mean PDI was registered in Remuga Keble (93.0%) and the lowest in Buyi Keble (58.3%). Similarly, the highest mean PSI was recorded in Buyi Keble (87.0%) and the lowest PSI (54.5%) was registered in Tawlla Keble. Among 60 seeds, Mareko local pepper inoculated by F. oxysporum f. sp. and R. solanacearum shows the highest susceptibility of 55 (91.0%) and 30 (50.0%), respectively. However, Mitmita local pepper was registered as the lowest susceptibility to both F. oxysporum f. sp. and R. solanacearum of 28.3% and 30.0%, respectively. Based on the finding, it can be concluded that pepper wilt was caused by a complex of fungus Fusarium oxysporum f. sp. capsici and bacteria Ralstonia solanacearum sp. in the study area. So, it is recommended that an integrated disease management approach should be implemented to manage the complex diseases of the site.


2014 ◽  
Vol 8 (3) ◽  
pp. 121-126
Author(s):  
REJEKI SITI FERNIAH ◽  
◽  
BUDI SETIADI DARYONO ◽  
RINA SRI KASIAMDARI ◽  
ACHMADI PRIYATMOJO

2018 ◽  
Vol 29 (2) ◽  
pp. 29-37
Author(s):  
Rejeki Siti Ferniah ◽  
◽  
Rina Sri Kasiamdari ◽  
Achmadi Priyatmojo ◽  
Budi Setiadi Daryono ◽  
...  

2018 ◽  
Vol 4 (2) ◽  
pp. 138
Author(s):  
Deswidya Sukrisna Hutauruk

This study was aims to determine the viability of chitinolytic bacterial isolates in some carrier media and to know the potential of chitinolytic bacterial isolates in various carrier media in controlling the disease of sprout caused by F. oxysporum and S. rolfsii on red pepper plant. The method used by measuring the viability and ability of bacteria in growing media and carrier media on MGMK media after storage of carrier media for 90 days. Bacteria NR09 has good viability on peat carrier media and palm oil bunch compost  and has the potential to inhibit attack of pathogenic fungi S. rolfsii and F. oxysporum.


2015 ◽  
Vol 9 (3) ◽  
pp. 142-147
Author(s):  
Rejeki Siti Ferniah ◽  
Rina Sri Kasiamdari ◽  
Achmadi Priyatmojo ◽  
Budi Setiadi Daryono

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