EFFECTIVENESS TEST OF BLACK CUMIN SEEDS (NIGELLA SATIVA) EXTRACT ON THE GROWTH OF KLEBSIELLA PNEUMONIAE AND PSEUDOMONAS AERUGINOSA BACTERIA

Black cumin seeds are known to have beneficial contents as antibacterial. This study aimed to determine the effectiveness of black cumin seed extract (Nigella sativa) on the growth of Klebsiella pneumoniae and Pseudomonas aeruginosa. The method used was the disc diffusion method. The ethanol extract of black cumin seeds gain by maceration method and was made into various concentration (5, 7.5, 10, 12.5, and 15)%. For positive control, ciprofloxacin disc were used and negative control used distilled water. The research model used was in vitro experimental research. The results showed that ethanol extract of black cumin seeds have antibacterial activity against Klebsiella pneumoniae with inhibition zone diameters (4.5, 6.7, 10.1, 11, 12.8) mm, Pseudomonas aeruginosa with inhibition zone diameters (7.5, 12, 12.6, 13.4, 15.6) mm, positive controls respectively (24.3 and 20.7) mm and negative controls (0). It can be concluded that black cumin seed extract has an inhibiting effect on the growth of Klebsiella pneumoniae and Pseudomonas aeruginosa.

GLANDULAR TRICHOME IN THE ASTERACEAE FAMILY

The Asteraceae is a diverse plant species and widely distributed, especially in the tropics and subtropics, consisting of 1,600 - 1,700 genera which include 24,000 - 30,000 species. Asteraceae has characteristics of cup flowers and brackets that are not owned by other plants. The objective of this study was to determine the morphological structure of plants and escpecially the leaf glandular trichome in several species of Asteraceae. The research method used was a descriptive method, to describe and interpret the shape, structure and distribution of leaf trichome in the Asteraceae family. This study used eight species of the Asteraceae family, namely Elephantopus mollis, Bidens pilosa, Tithonia deversifolia, Tridax procumbens, Synedrella nodiflora, Eclipta prostrate, Sphagneticola trilobata and Ageratum conyzoides. The observation results of trichomes at 8 species by Scanning Electrone Microscope (SEM) was obtained varied forms of trichomes, both in shape and size. From the research, it was obtained that the forms of multicellular glandular trichome with various shapes, ranging in size from 50.6 µm - 831.9 µm.

ESTIMATION OF NUMBERS OF COLIFORM BACTERIAL AS WATER QUALITY INDICATOR IN KEPAHIANG DISTRICT RIVERS, BENGKULU PROVINCE

Coliform is a group of microbes that are used as indicators of water quality. Water pollution is generally caused by pathogenic microbes from feces, household waste, and industrial activity waste. This study aimed to estimate the total number of coliform contamination in several rivers in Kepahiang Regency, namely Tebat Monok (TM), Sempiyang (SPY), Penanjung Panjang (PP), Embong Ijok (EI) Air Langkap(ALK), and Air Belimbing (ABB). Total coliform and Fecal coliform tests were carried out using the Most Probable Number (MPN) method on Lactose Broth, Brillian Green Lactose Bile Broth and pour plates on Eosin Methylene Blue Agar media. Measurement of abiotic factors was on temperature and pH parameters. The test results of total coliform showed that 6 rivers contained total coliform under the Class II river water quality standards with a range of 1210/100 mL– 4310/100 mL and 2 rivers that were contaminated with Fecal coliform, TM and ALK, have the content of 1500/100 mL and 1700 / 100 mL. The results of the measurement of the abiotic factor, the river pH range was 7.4 - 8.2. The lowest temperature was 25oC in SPY river and the highest temperature was 26 oC on the TM, PP, EI, ALK, and ABB rivers.

THE EFFECT OF COCOPEAT AND RICE HUSK PLANTING MEDIA HYDROPONICALLY ON THE GROWTH OF PALM OIL IN PRE NURSERY

Palm oil can produce high productivity which must be supported from several aspects, one of them is a good and correct nursery. Palm oil nursery isusually carried out conventionally, namely planting using soil media. Hydroponic research using cocopeat and rice husks planting media on the growth of palm oil seeds in pre nursery has never been carried out. The objective of this study was to determine the growth of palm oil seedlings using the hydroponic method. This research was conducted in the STIPAP Medan greenhouse. This research was conducted from April to July 2018. This research was a non-factorial RAK, which consisted of four treatments, namely M0 (top soil), M1 (Cocopeat), M2 (rice husks), M3 (combination). Height, stem, number of leaves, dry and wet weight of roots and plants, root length were observed. Then data analysis with ANOVA with the continued test of 5% Duncan was performed. The results of the effect of cocopeat and rice husk media was there was no a significant effect on the treatment parameters of seed height, number of leaves, dry and wet weight of roots and plants, and root length.

TEMPERATURE AND NITRATE CONCENTRATION EFFECT ON THE ABUNDANCE AND GROWTH RATE OF MELOSIRA SP.

Microalgae are one of the natural resources that have high potential as a source of biofuels, one of them is the marine microalgae Melosira sp.. The objective of this study was to determine the effect of increasing temperature and nitrate concentration on the abundance, growth rate, biomass, organic C-content and free fatty acids of Melosira sp.. A Completely Randomized Design was used for this experiment with four temperature treatments and three nitrate concentrations treatments in microalgae test planting media. The test microalgae were cultured for 21 days, while the abundance of Melosira sp..was carried out every 3rd day, while for other parameters measurements were made at the end of the experiment. The results showed that the increasing in temperature and nitrate concentration in culture media significantly affected the abundance of Melosira sp.. Meanwhile, the results of statistical analysis showed that the increase in temperature and concentration of culture media did not have a significant effect on biomass except at 30°C treatment, the dry weight of Melosira sp. was only significantly different between the nitrate concentrations of 0.0 g/L and 0.4 g/L. For the content of C-organic and free fatty acids generally showed a significant difference between treating temperature and nitrate concentration in the culture media.

ETHNOMEDICINE OF BAJO TRIBE COMMUNITY IN BANGKO VILLAGE, MAGINTI DISTRICT, WEST MUNA, SOUTHEAST SULAWESI

This study aims to reveal the use of marine and coastal plants, and animals as medicine by Bajo Tribe society in Bangko Village, West Muna. The method used was an exploratory survey. Data were collected using in-depth interviews with 20 traditional healers selected by purposive sampling. The data obtained were analyzed descriptively. The result revealed that there were 39 species of plants and 5 species of animals used as medicine. The most widely used plant families were the Arecaceae and Poaceae, 3 species respectively, while animals from the families of Holothuriidae, Onuphidae, Pinnidae, Syngnathidae, and Tridacnidae, 1 species respectively. The most commonly part of plant used was leaves (50%), while for animal was parts of flesh and whole body were used 40% respectively. The most commonly used preparation and application were boiling and drinking. In conclusion, Bajo people in Bangko Village with their maritime culture use plants and animals in the marine and coastal environment to overcome various diseases and health problems, in addition using “baca-baca” method.

MACROFUNGI DIVERSITY IN MOUNT BURNI TELONG BENER MERIAH REGENCY ACEH PROVINCE

Mount Burni Telong has a diversity of fungi that play an important role in the forest ecosystem. This study aimed to determine the types of macrofungi found in Mount Burni Telong and index diversity macrofungi found in Mount Burni Telong. The method used in this study was to use the pathway method by observing macrofungi along the hiking trail with a distance of 10 meters to the left and 10 meters to the right starting from an altitude of 1800 mdpl to an altitude of 2600 mdpl. Macroscopic fungi found were to identified and measured the physico-chemical parameters of the environment. Macroscopic fungi found were dominated by the Basidiomycota division and the Polyporaceae family. The results obtained were 31 macroscopic fungi from 2 divisions, 6 classes, 6 orders, 21 families, 26 genera, and 31 species. The most macroscopic fungi found in the Basidiomycota division, Agaricomycetes class, were 23 species and at least in the Ascomycota division, Pezizomycetes class, was as many as 1 species.

DETERMINATION OF THE TROPICAL STATUS OF FLOATING NET CAGE WATER BASED ON THE DISTRIBUTION OF NITROGEN, PHOSPHORUS AND CHLOROPHYLL-A

The dominant source of pollutants for floating net cages are fish feed and feces. They cause phosphorus and nitrogen in water increase, trigger eutrophication, marked by the appearance of algae. Algae are green plants, contain chlorophyll-a. The content of phosphorus, nitrogen and chlorophyll-a can be used to determine the tropical status of water. The objective of this research is to determine the tropical status of marine cage water. The research method was descriptive laboratory. Nitrogen content is measured as nitrite according to SNI 6989.9-2004, nitrate according to APHA Section 4500-NO3, ammonia according to SNI 19-1655-1989. Phosphorus analysis according to SNI 06-6989-31: 2005. Chlorophyll-a analysis used the Strickland & Parson method by spectrophotometry. Laboratory data were analyzed for tropical status based on nitrogen, phosphorus and chlorophyll-a content. The results showed that the tropical status of the KJA water of Gajah Mungkur Reservoir Wonogiri in the rainy season had eutrophic status, containing high levels of phosphorus and nitrogen elements. The eutrophic status indicated that the water had been polluted by an enhancement of nitrogen levels by 18.345 µg/L and phosphorus by 420.65 µg/L. These nitrogen and phosphorus pollutants increased the growth of chlorophyll-a by 12.70 µg/L.

VEGETATION COMPOSITION ON ECOLOGICAL FUNCTION IN MATARAM MERAH PARK, JAKARTA

The existence of the city park is important for Jakarta as a metropolitan city with the current activity level of motor vehicles and air pollution greatly affects the quality of the city environment. The selection of vegetation in Mataram Merah park considered functional aspects and aesthetic values . The objective of this study were to determine the composition and type of vegetation landscape in Mataram Merah park and to determine the function of each type of landscape vegetation in Mataram Merah park. This study used a descriptive qualitative method with several stages, which is survey; identification of the name and type of vegetation; size identification; identification of the type of vegetation making up the composition (name of type and size that makes up the vegetation structure). The results showed that Mataram Merah park was generally planted with vegetation that functioned aesthetically rather than functionally. The function of landscape plants in Mataram Merah park were as aesthetics is 96.55%, as controlling view as much as 13.80%, vegetation function as physical barrier as much as 62.07%, plants as controlling erosion 51.72%, function of plants as controlling climate 13.79% and vegetation function for wildlife as much as 17.24%.

THE POTENTIAL OF NGOKILO LEAVES EXTRACT (STACHYTARPHETA MUTABILIS. VAHL) AS A LOWERING OF BLOOD GLUCOSE LEVELS OF WHITE MICE (RATTUS NORVEGICUS)

Diabetes is a disease, in which the body cannot produce enough insulin so that there is excess sugar in the blood which becomes toxic to the body. Ngokilo plants are believed to be able to lower blood sugar levels because of the antioxidants and polyphenols contained therein.The objective of this study was to determine the potential of the ethanol extract of Ngokilo leaves (Stachytarpheta mutabilis, Vahl) as a lowering blood glucose levels in white mice(Rattus norvegicus). The tests were carried out by an experimental method using Swiss Webster male mice (Rattus norvegicus) aged 2-3 months with a body west of 30-40 grams as test animals. This study used four dosage ratios of ngokilo leaf extract, namely 1: 20: 40: 60 and control used aquadest. The results showed that the ethanol extract of Ngokilo leaves had an F value of 27.033> F Table 2.60. It indicates that the independent variable (the difference in the concentration of ethanol extract of Ngokilo leaves) was proven to have a significant effect on the dependent variable (blood sugar levels).