The purpose of this research was to create new flax genotypes resistant to anthracnose using biotechnological techniques and methods. As a result of studies with using a culture of immature embryos and a selective medium, flax regenerant plants resistant to the culture filtrate (CF) of the fungus - anthracnose pathogen Colletotrichum lini Manns et Bolley and line 21 resistant to this pathogen were obtained. A scheme for differentiating flax genotypes in vitro by resistance to anthracnose has been developed. It was established that upon cultivation of immature embryos on Sh-2 medium containing CF of the fungus, the causative agent of anthracnose, of a mixture of strains 680, 677 * 674, 674 * at a concentration of 36.0 ml / l, the amount of morphogenic callus formed in the first and second passages, expressed in percent, and the indicator of the field resistance of this genotype to anthracnosis on an artificial infectious-provocative background was close in value, and the number of morphogenic callus formed in the first and second passages can be used to judge the resistance of the studied genotypes to ant cancer and differentiate them by resistance to this pathogen. The influence of the flax genotype on the potency of cells to morphogenesis under selective conditions was revealed. Cells of genotypes L 957-8-7, Alexim, Punjab, Zaryanka had high morphogenetic activity. The morphogenetic potential of genotypes L 1506-8-4, Rosinka has already been exhausted by 2 ... 3 passages. It was found that biotechnological methods: cell selection in vitro, embryo culture are effective in creating genotypes of flax, more resistant to anthracnose than original forms.
