Clone selection of in vitro horseradish hairy root cultures

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
R Bertóti ◽  
Á Alberti ◽  
A Böszörményi ◽  
R Könye ◽  
T Horváth ◽  
...  
Keyword(s):  
Hairy Root ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Clone Selection ◽  
Selection Of

scholarly journals Selection of reference genes for qPCR in hairy root cultures of peanut

2011 ◽  
Vol 4 (1) ◽  
Author(s):  
Jose Condori ◽  
Cesar Nopo-Olazabal ◽  
Giuliana Medrano ◽  
Fabricio Medina-Bolivar
Keyword(s):  
Hairy Root ◽  
Reference Genes ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Selection Of

A Protocol for the Selection of Spontaneous Variants from Established Catharanthus roseus Hairy Root Cultures

2020 ◽  
pp. 65-75
Author(s):  
Felipe A. Vázquez-Flota ◽  
María de Lourdes Miranda-Ham ◽  
Marina del Pilar Navarrete-Loeza ◽  
Miriam Monforte-González
Keyword(s):  
Hairy Root ◽  
Catharanthus Roseus ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Selection Of

Hepatoprotective Triterpenes from Hairy Root Cultures of Ocimum basilicum L.

2009 ◽  
Vol 64 (3-4) ◽  
pp. 201-209 ◽  
Author(s):  
Amani M. Marzouk
Keyword(s):  
Oxidative Stress ◽  
Hairy Root ◽  
Liver Homogenate ◽  
Hepatoprotective Activity ◽  
Ocimum Basilicum ◽  
Albino Rats ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Oxidative Stress Status

Six triterpene acids identified as betulinic, oleanolic, ursolic, 3-epimaslinic, alphitolic and euscaphic acids have been isolated from a dichloromethane extract of hairy root cultures of Ocimum basilicum L. (Lamiaceae). These cultures were obtained by genetic transformation using Agrobacterium rhizogenes. The extract as well as the isolated compounds were evaluated for their hepatoprotective activity by measuring their effect on the oxidative stress status of liver, induced by carbon tetrachloride, in albino rats and in liver homogenate in vitro. All tested compounds displayed hepatoprotective activity comparable to oleanolic and ursolic acids.

Epiallo-yohimbine derivatives isolated from in vitro hairy-root cultures of Catharanthus trichophyllus

1989 ◽  
Vol 28 (5) ◽  
pp. 1383-1387 ◽  
Author(s):  
Elisabeth Davioud ◽  
Christiane Kan ◽  
Jean-Charles Quirion ◽  
Bhupesh C. Das ◽  
Henri-Philippe Husson
Keyword(s):  
Hairy Root ◽  
Hairy Root Cultures ◽  
Root Cultures

scholarly journals Alterations in oleanolic acid and sterol content in marigold (Calendula officinalis) hairy root cultures in response to stimulation by selected phytohormones

2021 ◽  
Vol 43 (3) ◽  
Author(s):  
Abdulwadood S. M. Alsoufi ◽  
Klaudia Staśkiewicz ◽  
Michał Markowski
Keyword(s):  
Oleanolic Acid ◽  
Hairy Root ◽  
Hairy Roots ◽  
Naphthaleneacetic Acid ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Calendula Officinalis ◽  
Plant Metabolites ◽  
Triterpenoid Saponins

AbstractHairy root cultures are an efficient tool for the biotechnological production of plant metabolites and a convenient experimental model for analyzing the effect of various compounds on plant metabolism. In contrast to many other types of in vitro plant cultures, hairy roots do not require an external supply of phytohormones to the medium. Consequently, plant growth regulators such as auxins and cytokinins are rarely used as elicitors in hairy root in vitro cultures; however, they can strongly influence plant defense responses. The aim of this study was to investigate the influence of two auxins: natural indole-3-acetic acid (IAA) and synthetic 1-naphthaleneacetic acid (NAA), as well as two cytokinins: natural kinetin and synthetic 6-benzylaminopurine (BAP) at a concentration of 0.75 mg/L on the metabolism of sterols and triterpenoids in Calendula officinalis hairy roots. Auxins prevented the accumulation of triterpenoid saponins (oleanolic acid glycosides), while cytokinin BAP increased their accumulation by 17% and their release into the culture medium by a factor of 10. Other cytokinins and kinetins increased the sterol levels by 17%, the level of stigmasterol by 15%, and the level of isofucosterol by 7 times.

scholarly journals Production of hairy root cultures of lettuce (Lactuca sativa L.)

2014 ◽  
Vol 9 (12) ◽  
pp. 1196-1205 ◽  
Author(s):  
Tadić Vojin ◽  
Milošević Snežana ◽  
Cingel Aleksandar ◽  
Petrić Marija ◽  
Trifunović Milana ◽  
...  
Keyword(s):  
Hairy Root ◽  
Lactuca Sativa ◽  
Target Genes ◽  
Pcr Analysis ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Lactuca Sativa L ◽  
Half Strength ◽  
Independent Transformation

AbstractHairy root cultures of lettuce (Lactuca sativa L.) were obtained by inoculation of cotyledonary leaves of in vitro lettuce seedlings (cvs. Nansen and Ljubljanska ledenka) with Agrobacterium rhizogenes A4M70GUS. Approximately in 96.7% cvs. Nansen and in 91.2% Ljubljanska ledenka inoculated explants produced hairy root when they were incubated on Murashige and Skoog (MS) half-strength medium without plant growth regulators. A total of 54% of all hairy root cultures expressed GUS activity. Every hairy root represented an independent transformation event. Line Ljubljanska ledenka 18 showed the highest biomass (5.5 times the biomass of control root). A PCR analysis of the genomic DNA confirmed the presence of marker and target genes in 15 hairy roots examined.

scholarly journals Influence of Selected Abiotic Factors on Triterpenoid Biosynthesis and Saponin Secretion in Marigold (Calendula officinalis L.) in Vitro Hairy Root Cultures

Molecules ◽  
2019 ◽  
Vol 24 (16) ◽  
pp. 2907 ◽  
Author(s):  
Abdulwadood Shakir Mahmood Alsoufi ◽  
Cezary Pączkowski ◽  
Marek Długosz ◽  
Anna Szakiel
Keyword(s):  
Hairy Root ◽  
Abiotic Factors ◽  
Sterol Composition ◽  
Root Tissue ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Calendula Officinalis ◽  
Pentacyclic Triterpenoids ◽  
Uv C

The aim of the study was the evaluation of the efficiency of selected abiotic elicitors, i.e., silver and cadmium ions, ultrasound, and UV-C irradiation, in the stimulation of triterpenoid biosynthesis, accumulation, and saponin secretion in Calendula officinalis hairy root cultures. Apart from the possible enhancement of triterpenoid production, the relationship between primary and secondary metabolism (represented respectively by sterols and pentacyclic triterpenes), modifications of the sterol compositional profile, and fluctuations in the total triterpenoid content were monitored in the performed experiments. The main phenomenon observed as a response to heavy metal treatment was the stimulation (up to 12-fold) of the secretion of saponins, accompanied by significant changes in sterol composition. Ultrasound stimulated the secretion of saponins (up to 11-fold); however, it exerted diverse influences on the triterpenoid content in hairy root tissue (stimulating or decreasing) depending on the duration of the exposure to the elicitor. UV-C radiation caused a slight increase in the content of both sterols and saponins in hairy root tissue, and stimulated saponin secretion up to 8.5-fold. The expected symptoms of the competition between the biosynthetic pathways of sterols and pentacyclic triterpenoids were less evident in reactions to abiotic stressors than those reported previously for biotic elicitors.

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