Proline-U-14C injected into the endosperm of 3-day-old etiolated seedlings of Montcalm barley and its mutant was readily translocated to the embryo. Of label present in the embryo, over 65% was accounted for by protein and free amino acids and about 25% was present in organic acids. Proline accounted for over 90% of the label in free amino acids of the endosperm but accounted for less than 50% of label in this fraction in the embryo. Proline was readily converted to glutamate in the embryo. Metabolism of proline was similar in both lines.