IN VITRO-CULTURE INITIATION AND MICROPROPAGATION OF FEIJOA CULTIVAR ‘COOLIDGE’

The main aim of present study was to overcome the problems associated with the in vitro culture initiation in Terminalia arjuna. The micropropagation of tree species is not easy as shrubs and herbs. Many problems encountered from explant collection to in vitro culture establishment. The problems that have been occurred during T. arjuna micropropagation were culture contamination, phenolic exudation, bud growth inhibition, shoots yellowing and leaf fall. All these problems have been solved by applying certain treatments prior to explant collection and inoculation. The mother tree was lopped in November months (six months prior to explant collection) to remove any inhibitory substance and release bud growth. Different sterilizing agents were used to minimize the bacterial and fungal contamination. Some modification in culture media (use of different concentration of NH4NO3 and KNO3 salts and adenine sulphate) was done. Surface sterilization of nodal explants collected from lopped branches with 0.1% HgCl2 for 8 min., treatment with chilled antioxidant solution (Ascorbic acid, Citric acid and PVP) and half strength of NH4NO3 and KNO3 salts of MS medium supported 100% bud break response with proliferation of green and healthy in vitro shoots. Removing these hurdles already in the initial stage of micropropagation is very important and maximize mass in vitro propagation of this medicinally important Arjun tree.

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IN VITRO CULTURE INITIATION AND CRYOPRESERVATION OF ENDEMIC TAXA DIANTHUS GIGANTEUS SSP. BANATICUS

Acta Horticulturae ◽

10.17660/actahortic.2011.918.18 ◽

2011 ◽

pp. 153-159 ◽

Cited By ~ 3

Author(s):

L. Jarda ◽

V. Cristea ◽

A. Halmagyi ◽

M. Plada

Keyword(s):

In Vitro Culture ◽

Culture Initiation

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Management of stockplant for the in vitro culture of nodal segments of guava (Psidium guajava L.).

The Journal of Agriculture of the University of Puerto Rico ◽

10.46429/jaupr.v88i1-2.2736 ◽

1969 ◽

Vol 88 (1-2) ◽

pp. 73-89

Author(s):

Maribel Ramírez-Villalobos ◽

Silvia León de Sierralta ◽

Merylin Marín ◽

Alba Nava

Keyword(s):

In Vitro Culture ◽

Psidium Guajava ◽

Nodal Segments ◽

Control Treatment ◽

Culture Initiation ◽

Sun And Shade ◽

Adult Plants

Nodal segments collected from shoots of adult grafts on juvenile rootstocks from seedlings grown under full sun and shade (40%), and from adult plants grown without and with shade (80%) were cultivated in vitro. In addition, nodal segments from field grown plants were cultivated in vitro under the following conditions: control; sixty days after dormex (hydrogenated cyanamide 49%) application at 2% and 4%; sixty days after partial pruning; and 75 days of 80% shading. Sixty days after culture initiation, seedlings and adult plants under shade produced no browning explants. The highest percentage of budbreak (PBB) (100%) was obtained at 20 days in explants of seedlings; at 40 days in explants of adult plants under shade. At sixty days, adult and grafted plants without shade presented 40% and 35% browning explants, respectively, and 85% and 80% budbreak explants, respectively. At sixty days, the control treatment produced 65% budbreak; the dormex treatment with the concentrations of 2% and 4%, 80% and 70% PBB, respectively; the partial pruning, 65%. Dormex application at 2% increased PBB and percentage of leaves formed to more than that of the control.

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052 In Vitro Culture Initiation and Proliferation of Exochorda racemosa

HortScience ◽

10.21273/hortsci.35.3.397c ◽

2000 ◽

Vol 35 (3) ◽

pp. 397C-397

Author(s):

Guochen Yang ◽

Marihelen Kamp-Glass

Keyword(s):

In Vitro Culture ◽

Woody Plant ◽

Shoot Proliferation ◽

Deionized Water ◽

In Vitro Cultures ◽

Culture Initiation ◽

Woody Plant Medium ◽

Ornamental Shrub ◽

Ornamental Species

Exochorda racemosa is an ornamental shrub with white flowers that is spiraea-like, deciduous, and hardy. The buds resemble pearls. Normally it is propagated by seeds, layers, and cuttings of softwood. However, it is a slow process that takes a few years to produce a reasonable size plant for the demanding market. Our objective was to establish a successful in vitro culture and to rapidly multiply this ornamental species. Softwood explant materials were collected from a local nursery and were disinfested with 15% bleach solution and rinsed three times with sterile distilled and deionized water. In vitro cultures were established and maintained in woody plant medium (WPM) supplemented with BA at 0.1 mg·L-1, 3% sucrose, and 0.7% agar with the pH adjusted to 5.8. Then shoots were transferred to the multiplication medium containing BA, CPPU, or thidiazuron (TDZ) at various concentrations. Preliminary results show that explants cultured on medium containing TDZ produced the best shoot proliferation.

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In vitro culture initiation using immature seeds of a rare species Fritillaria meleagroides Patrin ex Schult. et Schult. fil. (Liliaceae)

Vestnik Tomskogo gosudarstvennogo universiteta Biologiya ◽

10.17223/19988591/44/10 ◽

2018 ◽

pp. 172-187 ◽

Cited By ~ 1

Author(s):

Dinara S. Muraseva ◽

◽

Tatyana I. Novikova ◽

Keyword(s):

In Vitro Culture ◽

Rare Species ◽

Culture Initiation ◽

Immature Seeds

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Rhizome Buds Disinfection for Preparation of Red Ginger (Zingiber officinale Roxb. var. rubrum Rosc.) In Vitro Culture

Media Pharmaceutica Indonesiana (MPI) ◽

10.24123/mpi.v3i1.2836 ◽

2020 ◽

Vol 3 (1) ◽

pp. 19-26

Author(s):

Popy Hartatie Hardjo ◽

Alfian Hendra Krisnawan

Keyword(s):

In Vitro Culture ◽

Axenic Culture ◽

Zingiber Officinale ◽

Culture Initiation ◽

Surface Sterilization ◽

Sterilization Method ◽

Ginger Rhizome ◽

Red Ginger ◽

Rhizome Buds

The success of culture initiation depends on explant surface sterilization techniques. Suitable concentration, combinations, and duration of exposure of sterilizing agents are important to raise in vitro culture successfully. The aim of this work is to obtain the suitable sterilization method for explant buds of red ginger rhizome to get the axenic culture. Four sterilizing agents, fungicide, bactericide, Cefotaxime antibiotic, and NaOCl were tested for sterilization by various concentration and duration of exposure. The results showed that sterilizing agents 200 mg/L Cefotaxime and 100 mg/L Benomyl combined with NaOCl decreased the contamination of explants, and achieved 20% axenic culture.

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