scholarly journals Management of stockplant for the in vitro culture of nodal segments of guava (Psidium guajava L.).

1969 ◽  
Vol 88 (1-2) ◽  
pp. 73-89
Author(s):  
Maribel Ramírez-Villalobos ◽  
Silvia León de Sierralta ◽  
Merylin Marín ◽  
Alba Nava
Keyword(s):  
In Vitro Culture ◽  
Psidium Guajava ◽  
Nodal Segments ◽  
Control Treatment ◽  
Culture Initiation ◽  
Sun And Shade ◽  
Adult Plants

Nodal segments collected from shoots of adult grafts on juvenile rootstocks from seedlings grown under full sun and shade (40%), and from adult plants grown without and with shade (80%) were cultivated in vitro. In addition, nodal segments from field grown plants were cultivated in vitro under the following conditions: control; sixty days after dormex (hydrogenated cyanamide 49%) application at 2% and 4%; sixty days after partial pruning; and 75 days of 80% shading. Sixty days after culture initiation, seedlings and adult plants under shade produced no browning explants. The highest percentage of budbreak (PBB) (100%) was obtained at 20 days in explants of seedlings; at 40 days in explants of adult plants under shade. At sixty days, adult and grafted plants without shade presented 40% and 35% browning explants, respectively, and 85% and 80% budbreak explants, respectively. At sixty days, the control treatment produced 65% budbreak; the dormex treatment with the concentrations of 2% and 4%, 80% and 70% PBB, respectively; the partial pruning, 65%. Dormex application at 2% increased PBB and percentage of leaves formed to more than that of the control.

scholarly journals Problems Encountered during In vitro Culture Establishment in Terminalia arjuna

2020 ◽  
pp. 154-160
Author(s):  
Meena Choudhary ◽  
Inder Dev Arya ◽  
Sarita Arya
Keyword(s):  
In Vitro Culture ◽  
Culture Media ◽  
Terminalia Arjuna ◽  
Culture Initiation ◽  
Surface Sterilization ◽  
Initial Stage ◽  
In Vitro Shoots ◽  
Half Strength ◽  
Bud Growth

The main aim of present study was to overcome the problems associated with the in vitro culture initiation in Terminalia arjuna. The micropropagation of tree species is not easy as shrubs and herbs. Many problems encountered from explant collection to in vitro culture establishment. The problems that have been occurred during T. arjuna micropropagation were culture contamination, phenolic exudation, bud growth inhibition, shoots yellowing and leaf fall. All these problems have been solved by applying certain treatments prior to explant collection and inoculation. The mother tree was lopped in November months (six months prior to explant collection) to remove any inhibitory substance and release bud growth. Different sterilizing agents were used to minimize the bacterial and fungal contamination. Some modification in culture media (use of different concentration of NH4NO3 and KNO3 salts and adenine sulphate) was done. Surface sterilization of nodal explants collected from lopped branches with 0.1% HgCl2 for 8 min., treatment with chilled antioxidant solution (Ascorbic acid, Citric acid and PVP) and half strength of NH4NO3 and KNO3 salts of MS medium supported 100% bud break response with proliferation of green and healthy in vitro shoots. Removing these hurdles already in the initial stage of micropropagation is very important and maximize mass in vitro propagation of this medicinally important Arjun tree. 

scholarly journals Introduction of Stevia rebaudiana Bertoni in in vitro culture

2020 ◽  
Author(s):  
S. Sh. Asadova
Keyword(s):  
Cell Culture ◽  
In Vitro Culture ◽  
High Speed ◽  
Stevia Rebaudiana ◽  
Stevia Rebaudiana Bertoni ◽  
A Cell ◽  
Different Levels ◽  
Adult Plants

A cell culture obtained from explants of adult plants and aseptic seedlings of the Stevia rebaudiana Bertoni variety with different levels of ploidy, characterized by high speed, proliferation and ability to morphogenesis.

scholarly journals In vitro Clonal Multiplication of Two Grape (Vitis spp.) Rootstock Genotypes

2018 ◽  
Vol 28 (1) ◽  
pp. 1-11 ◽  
Author(s):  
M Alizadeh ◽  
SK Singh ◽  
VB Patel ◽  
PS Deshmukh
Keyword(s):  
In Vitro Regeneration ◽  
Shoot Multiplication ◽  
Nodal Segments ◽  
Multiplication Rate ◽  
Culture Initiation ◽  
Single Node ◽  
Clonal Multiplication ◽  
Rooting Medium ◽  
In Vitro Performance

Single node segments were used to initiate in vitro cultures in two grape rootstocks namely, Dogridge (Vitis champini) and H-144 (Vitis vinifera × V. labrusca). Culture establishment was enhanced using different growth regulators, while BAP was found essential for culture initiation in both genotypes. Less success (38.31%) was obtained in culture establishment of H-144 but it exhibited better vegetative growth and rooting and ex vitro performance as compared to Dogridge. Higher shoot multiplication rate (12 micro-cuttings per culture) was recorded in H-144 while only 9 micro-cuttings per subculture were registered in Dogridge. Addition of activated charcoal to the rooting medium was found beneficial with enhancement of rooting and reduction in time to root initiation in both genotypes. The results suggested that multiplication of these two grape rootstock genotypes can be carried out efficiently by means of direct in vitro regeneration using nodal segments. In vitro performance of these two genotypes was also compared during different stages of micropropagation.Plant Tissue Cult. & Biotech. 28(1): 1-11, 2018 (June)

scholarly journals Multiple shoot formation in Hypericum perforatum L.and hypericin production

2003 ◽  
Vol 15 (1) ◽  
pp. 43-47 ◽  
Author(s):  
Eliane Romanato Santarém ◽  
Leandro Vieira Astarita
Keyword(s):  
Hypericum Perforatum ◽  
Secondary Compounds ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Nodal Segments ◽  
Proliferation Medium ◽  
Shoot Production ◽  
Leaf Differentiation ◽  
Adult Plants

Hypericum perforatum is a traditional medicinal plant with wound healing and antidepressive properties. Among the secondary compounds of interest is hypericin, a naphtodianthrone that seems to participate in the medicinal effects of this species. The aim of this work was to obtain an efficient micropropagation system of H. perforatum and to compare the hypericin content between in vitro and field-grown plants. Cultures were initiated from nodal segments of mature plants inoculated onto MS medium supplemented with 4.5 muM BA, kinetin, thidiazuron, individually or in combination with 0.05 muM NAA. Organogenic explants were observed on medium with either BA or kinetin alone or in combination of these with NAA. Subculture of organogenic explants onto the proliferation medium containing 4.5 muM BA promoted the organogenic response. The highest average of shoot production (52.6 shoots) was obtained on those explants induced in the presence of BA and NAA. Rooted plantlets were successfully acclimated. Analysis of hypericin contents showed that levels found in callus represented only 0.11 % of what was detected in adult plants, while shoots and leaves from in vitro plants showed similar hypericin levels to those found in the leaves of the field-grown plants, suggesting that the accumulation of this compound is related to leaf differentiation.

scholarly journals Micropropagation of Different Citrus Rootstocks Using WPM Medium Culture

2019 ◽  
Vol 11 (4) ◽  
pp. 136
Author(s):  
Reisane Teles Santiago ◽  
Karen Cristina Fialho dos Santos ◽  
Carlos Alberto da Silva Ledo ◽  
Abelmon da Silva Gesteira ◽  
Walter dos Santos Soares Filho ◽  
...  
Keyword(s):  
Experimental Design ◽  
In Vitro Culture ◽  
Analysis Of Variance ◽  
San Diego ◽  
Nodal Segments ◽  
Direct Regeneration ◽  
Cultivated Plants ◽  
Multiplication Process ◽  
Medium Culture

Micropropagation is a method that enables in vitro cloning of cultivated plants, irrespective of their potential to produce seeds and their polyembryony rate, guaranteeing genetic identity and absence of phytosanitary problems of the propagated plants if the multiplication process is conducted suitably. In this respect, citrus rootstocks of agronomic interest were micropropagated in Wood Plant Medium (WPM). The experiment was conducted in the municipality of Cruz das Almas, in the Recôncavo Baiano region of Bahia state, Brazil, with 10 rootstocks: ‘Indio’, ‘Riverside’ and ‘San Diego’ citrandarins, ‘Sunki Tropical’ mandarin, and the hybrids RL × TR-001, FRL × (RL × TR)-005, CSM × (RL × TR)-059, TRH-051, TRH-069 and CSM × TRBK-Colombia. Nodal segments of these genotypes, with approximate length of 1 cm, were used. The number of plants formed per explant was evaluated 180 days after inoculation. The experimental design was completely randomized, with ten treatments and 12 repetitions, the data obtained were submitted to analysis of variance (F-test) and the grouped means were analyzed by the Scott-Knott test, in both cases a 5% probability. In vitro culture of explants of the ten genotypes studied presented positive responses to micropropagation in the WPM medium, once the explants of all genotypes showed direct regeneration and formed plants. The citrandarin 'Indio' he stood out with the highest average of explants by plant (4.20).

scholarly journals Micropropagation of Morus cathayana through in vitro culture from local Bogor, West Java, Indonesia

2019 ◽  
Vol 11 (1) ◽  
pp. 18-22 ◽  
Author(s):  
YASINTA RATNA ESTI WULANDARI ◽  
MENWANGI ADRIANASHINTA HARJOSUDIRJO
Keyword(s):  
In Vitro Culture ◽  
Shoot Growth ◽  
Nodal Segments ◽  
Statistical Data Analysis ◽  
Juvenile Period ◽  
West Java ◽  
Culture Techniques ◽  
Morus Spp ◽  
Tissue Culture Techniques

Wulandari YRE, Harjosudirjo MA. 2019. Micropropagation of Morus cathayana through in vitro culture from local Bogor, West Java, Indonesia. Nusantara Bioscience 11: 18-22. Mulberry (Morus spp.) is a dicotyledonous plant known for its medicinal benefits as well as silkworm breeding (Bombyx mori L.) to produce silk. Morus cathayana mainly cultivated due to its high content of 1-deoxynojirimycin, widely utilized as an anti-diabetic agent. However, common practice in planting and maintenance of mulberry generate less profit, contributed to its long juvenile period and high heterozygosity level. Hence the development and cultivation through plant tissue culture techniques are necessary. The purpose of this research was to obtain the best combination of thidiazuron as a plant growth regulator. M. cathayana branches with nodal segments were used as explants. Research stages were explants initiation into Murashige and Skoog media, shoot induction in MS media as control and MS+BAP 1 mg/L+NAA 0.25 mg/L+TDZ 0.1, 0.5, 1 mg/L as treatment media, and statistical data analysis. Greater increase in shoot growth was observed in MS+BAP 1 mg/L+NAA 0.25 mg/L+TDZ 0.5 mg/L media, while the formation of shoots and calluses on the explants grown in control and treatment media showed no significant growth.

scholarly journals EFFECT OF EXPALNT TYPE AND SOME PLANT GROWTH REGULATORS ON CULTURE INITIATION OF STEVIA PLANTS IN VITRO

2017 ◽  
Vol 48 (5) ◽  
Author(s):  
Khierallah & Al-Obaidy
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Shoot Tips ◽  
Control Treatment ◽  
Ms Medium ◽  
Explant Type ◽  
Culture Initiation ◽  
The Impact

This research was conducted in order to study the effect of explant type and some plant growth regulators on culture initiation of Stevia rebaudiana Bertoni in vitro. The experiments included surface sterilization and test two types of explants (shoot tips and stem nodes) and the impact of KIN and BA and IAA and IBA in the cultures initiation. Results revealed the efficiency of sodium hypochlorite (NaOCl) for disinfestation of explant at 0.050% concentration giving less contamination for shoot tips and stem nods (10% and 20% respectively). Results showed that shoot tips inoculated in MS medium plus KIN at 0.3 mg. L-1 was significantly increase the number of regenerated shoots as it produced 4.2 shoots per explant while medium without cytokinin (control) produced less number of shoots reached 1.4 shoots per explant. KIN treatment reduced shoots length as control treatment produced the highest length (6.74 cm).  The interaction between the explant type and BA concentration was significantly increase the number of regenerated shoots as shoot tips produced 3.6 shoots per explant in MS medium supplemented with 0.1 mg. L-1. BA treatment reduced shoots length as control treatment produced the highest length (6.74 cm). No positive effect was gain when auxins (IBA and IAA) were added in combination with cytokinin in culture medium. The above results can be adopted to established stevia in vitro culture successfully.

scholarly journals In vitro culture and diversity of endophytic fungi in Bambusa oldhamii

2019 ◽  
Vol 49 ◽  
Author(s):  
Ana Paula de Azevedo Pasqualini ◽  
Mariely Cristine dos Santos ◽  
Bruno Francisco Sant´Anna-Santos ◽  
Hugo Pacheco de Freitas Fraga ◽  
Marguerite Quoirin
Keyword(s):  
In Vitro Culture ◽  
Molecular Identification ◽  
Endophytic Fungi ◽  
Large Scale ◽  
Culture Medium ◽  
Current Knowledge ◽  
Nodal Segments ◽  
Scale Production ◽  
Bambusa Oldhamii

ABSTRACT Bambusa oldhamii Munro is a fast-growing species of woody bamboo with strong commercial appeal. In Brazil, the use of this species is limited, mainly due to the low availability of seedlings for commercial plantations. Micropropagation is a technique used for the large scale production of seedlings, but protocols for the establishment of aseptic cultures are hampered by the presence of endophytic contamination. This study aimed to develop an in vitro establishment protocol for B. oldhamii, as well as to make the molecular identification of fungi associated with the explants used. Nodal segments of adult plants grown in the field were used as explants. This material was submitted to two experiments carried out to evaluate the effect of 6-benzylaminopurine (BAP) on multiplication and of Plant Preservative Mixture (PPMTM) as a disinfectant. In the first one, 10 µM, 15 µM or 20 µM of BAP were combined with 1 mL L-1, 2 mL L-1 or 3 mL L-1 of PPMTM; while the second one used 0 µM, 2.5 µM, 5 µM or 7.5 µM of BAP with 4 mL L-1 of PPMTM, both added to MS culture medium. After 21 days of culture, the use of 4 mL L-1 of PPMTM inhibited the bacterial growth and reduced fungal contamination. The addition of BAP to the culture medium above 10 µM inhibited the formation and growth of new shoots, while additions of less than 7,5 µM had no effect. The molecular identification of the endophytic fungi isolated during the in vitro culture indicated the presence of numerous fungal species, increasing the current knowledge about the diversity of fungi associated with bamboo.

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