scholarly journals In vitro culture initiation using immature seeds of a rare species Fritillaria meleagroides Patrin ex Schult. et Schult. fil. (Liliaceae)

2018 ◽  
pp. 172-187 ◽  
Author(s):  
Dinara S. Muraseva ◽  
◽  
Tatyana I. Novikova ◽  
Keyword(s):  
In Vitro Culture ◽  
Rare Species ◽  
Culture Initiation ◽  
Immature Seeds

scholarly journals In vitro culture as an aid to conservation of indigenous ferns: Diplazium proliferum

2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Zubeir M. Golamaully ◽  
Vishwakalyan Bhoyroo ◽  
Nadeem Nazurally ◽  
Vineshwar Gopal
Keyword(s):  
Growth Rate ◽  
In Vitro Culture ◽  
Mercuric Chloride ◽  
Rare Species ◽  
Vascular Plants ◽  
Culture Media ◽  
Fungal Contamination ◽  
Increase Growth Rate ◽  
Growing Population

With the ever growing population and economic needs of Mauritius, the flora of Mauritius has never been in more danger and one group of vascular plants is even more in peril; ferns.<em> Diplazium proliferum</em> is indigenous to the Mascarene region and is considered as a rare species in Mauritius. The need to develop a tested <em>in vitro</em> propagation protocol is a must to protect the biodiversity of Mauritius. This experiment was geared towards the establishment of a proper sterilization technique and the effect of 6-benzylaminopurine (BAP) and light on <em>in vitro</em> culture of this fern. Sterilization with 0.05% Mercuric chloride was effective to eliminate fungal contamination and allow germination of spores. Culture media supplemented with BAP did not significantly increase growth rate of both gametophytes and sporophytes of<em> D. proliferum</em>. Present results suggest efficient sterilization methods to be a crucial stage for successful<em> in vitro r</em>egeneration of ferns. The established protocol will be used as an optimized baseline protocol for the propagation of other indigenous ferns.

scholarly journals Problems Encountered during In vitro Culture Establishment in Terminalia arjuna

2020 ◽  
pp. 154-160
Author(s):  
Meena Choudhary ◽  
Inder Dev Arya ◽  
Sarita Arya
Keyword(s):  
In Vitro Culture ◽  
Culture Media ◽  
Terminalia Arjuna ◽  
Culture Initiation ◽  
Surface Sterilization ◽  
Initial Stage ◽  
In Vitro Shoots ◽  
Half Strength ◽  
Bud Growth

The main aim of present study was to overcome the problems associated with the in vitro culture initiation in Terminalia arjuna. The micropropagation of tree species is not easy as shrubs and herbs. Many problems encountered from explant collection to in vitro culture establishment. The problems that have been occurred during T. arjuna micropropagation were culture contamination, phenolic exudation, bud growth inhibition, shoots yellowing and leaf fall. All these problems have been solved by applying certain treatments prior to explant collection and inoculation. The mother tree was lopped in November months (six months prior to explant collection) to remove any inhibitory substance and release bud growth. Different sterilizing agents were used to minimize the bacterial and fungal contamination. Some modification in culture media (use of different concentration of NH4NO3 and KNO3 salts and adenine sulphate) was done. Surface sterilization of nodal explants collected from lopped branches with 0.1% HgCl2 for 8 min., treatment with chilled antioxidant solution (Ascorbic acid, Citric acid and PVP) and half strength of NH4NO3 and KNO3 salts of MS medium supported 100% bud break response with proliferation of green and healthy in vitro shoots. Removing these hurdles already in the initial stage of micropropagation is very important and maximize mass in vitro propagation of this medicinally important Arjun tree. 

scholarly journals How Carbon Source and Seedcoat Influence the In Vitro Culture of Peach (Prunus persica L. Batsch) Immature Seeds

HortScience ◽  
2021 ◽  
pp. 1-2
Author(s):  
Margarita Pérez-Jiménez ◽  
Alfonso Guevara-Gázquez ◽  
Antonio Carrillo-Navarro ◽  
José Cos-Terrer
Keyword(s):  
Seed Germination ◽  
Carbon Source ◽  
In Vitro Culture ◽  
Seedling Growth ◽  
Woody Plant ◽  
Prunus Persica ◽  
In Vitro Germination ◽  
Immature Seeds ◽  
Woody Plant Medium

The effects of carbon source and concentration and of seedcoat were tested on the in vitro germination of peach seeds derived from crosses performed in the field. Seeds were extracted from the fruit and cultured in Woody Plant Medium (WPM) supplemented with sucrose, glucose, or sorbitol at concentrations of 15, 30, and 45 g·L−1. The percentage of germination as well as the root and hypocotyl lengths were measured after the stratification process and before acclimatization. Seedcoat did not have any influence on seed germination in any tested media and genotype. Glucose at a concentration of 15 g·L−1 and sucrose at 15, 30, and 45 g·L−1 resulted in greater stem seedling growth. The root developed the most when seeds were cultured in media with 15 or 30 g·L−1 of sucrose.

In situ seed development and in vitro regeneration of three difficult-to-propagate Lepidosperma species (Cyperaceae)

2010 ◽  
Vol 58 (2) ◽  
pp. 107 ◽  
Author(s):  
Andrea Kodym ◽  
Shane Turner ◽  
John Delpratt
Keyword(s):  
In Vitro Culture ◽  
In Vitro Regeneration ◽  
Field Studies ◽  
Fruit Production ◽  
Germination Response ◽  
Immature Seeds ◽  
Short Period ◽  
Viable Seeds

Field studies of fruit production from Lepidosperma concavum R.Br., L. laterale R.Br. and L. longitudinale Labill. showed that large proportions (21–77%) of fruits were unfilled and that filled and unfilled fruits looked alike. Bagging of inflorescences demonstrated that filled fruits tended to be shed, while empty fruits remained within the inflorescence. Time of collection was critical for obtaining viable seeds, with successful harvesting limited to a short period (weeks) after maturation. The timing of flowering and fruit maturation were fairly consistent between species, populations and years in our study area. In L. concavum fruit production was increased in cultivation compared with wild populations. In all three species, very little or no germination of fruits occurred under nursery conditions. In vitro culture initiation was attempted using intact fruits, nicked fruits and seeds on 1/2MS (Murashige and Skoog) medium with 1 µM zeatin and 0.5 µM gibberellic acid in darkness. Culture of intact fruit resulted in no germination, while nicked fruit showed some germination response. Best results were achieved from seeds with germination occurring as early as 7 to 18 days depending on the species. Germination of L. concavum, L. laterale and L. longitudinale was 86%, 64% and 83% respectively within 5 weeks. Germination response was strongly influenced by seed maturity. Mature seeds germinated significantly faster than immature seeds. On a small proportion of cultured seeds, calli formed and differentiated into numerous plantlets on growth regulator-free medium. Given the promising results observed in this study, in vitro culture appears to be a practical means of mass propagating Lepidosperma species.

scholarly journals Management of stockplant for the in vitro culture of nodal segments of guava (Psidium guajava L.).

1969 ◽  
Vol 88 (1-2) ◽  
pp. 73-89
Author(s):  
Maribel Ramírez-Villalobos ◽  
Silvia León de Sierralta ◽  
Merylin Marín ◽  
Alba Nava
Keyword(s):  
In Vitro Culture ◽  
Psidium Guajava ◽  
Nodal Segments ◽  
Control Treatment ◽  
Culture Initiation ◽  
Sun And Shade ◽  
Adult Plants

Nodal segments collected from shoots of adult grafts on juvenile rootstocks from seedlings grown under full sun and shade (40%), and from adult plants grown without and with shade (80%) were cultivated in vitro. In addition, nodal segments from field grown plants were cultivated in vitro under the following conditions: control; sixty days after dormex (hydrogenated cyanamide 49%) application at 2% and 4%; sixty days after partial pruning; and 75 days of 80% shading. Sixty days after culture initiation, seedlings and adult plants under shade produced no browning explants. The highest percentage of budbreak (PBB) (100%) was obtained at 20 days in explants of seedlings; at 40 days in explants of adult plants under shade. At sixty days, adult and grafted plants without shade presented 40% and 35% browning explants, respectively, and 85% and 80% budbreak explants, respectively. At sixty days, the control treatment produced 65% budbreak; the dormex treatment with the concentrations of 2% and 4%, 80% and 70% PBB, respectively; the partial pruning, 65%. Dormex application at 2% increased PBB and percentage of leaves formed to more than that of the control.

scholarly journals 052 In Vitro Culture Initiation and Proliferation of Exochorda racemosa

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 397C-397
Author(s):  
Guochen Yang ◽  
Marihelen Kamp-Glass
Keyword(s):  
In Vitro Culture ◽  
Woody Plant ◽  
Shoot Proliferation ◽  
Deionized Water ◽  
In Vitro Cultures ◽  
Culture Initiation ◽  
Woody Plant Medium ◽  
Ornamental Shrub ◽  
Ornamental Species

Exochorda racemosa is an ornamental shrub with white flowers that is spiraea-like, deciduous, and hardy. The buds resemble pearls. Normally it is propagated by seeds, layers, and cuttings of softwood. However, it is a slow process that takes a few years to produce a reasonable size plant for the demanding market. Our objective was to establish a successful in vitro culture and to rapidly multiply this ornamental species. Softwood explant materials were collected from a local nursery and were disinfested with 15% bleach solution and rinsed three times with sterile distilled and deionized water. In vitro cultures were established and maintained in woody plant medium (WPM) supplemented with BA at 0.1 mg·L-1, 3% sucrose, and 0.7% agar with the pH adjusted to 5.8. Then shoots were transferred to the multiplication medium containing BA, CPPU, or thidiazuron (TDZ) at various concentrations. Preliminary results show that explants cultured on medium containing TDZ produced the best shoot proliferation.

Rhizome Buds Disinfection for Preparation of Red Ginger (Zingiber officinale Roxb. var. rubrum Rosc.) In Vitro Culture

2020 ◽  
Vol 3 (1) ◽  
pp. 19-26
Author(s):  
Popy Hartatie Hardjo ◽  
Alfian Hendra Krisnawan
Keyword(s):  
In Vitro Culture ◽  
Axenic Culture ◽  
Zingiber Officinale ◽  
Culture Initiation ◽  
Surface Sterilization ◽  
Sterilization Method ◽  
Ginger Rhizome ◽  
Red Ginger ◽  
Rhizome Buds

The success of culture initiation depends on explant surface sterilization techniques. Suitable concentration, combinations, and duration of exposure of sterilizing agents are important to raise in vitro culture successfully. The aim of this work is to obtain the suitable sterilization method for explant buds of red ginger rhizome to get the axenic culture. Four sterilizing agents, fungicide, bactericide, Cefotaxime antibiotic, and NaOCl were tested for sterilization by various concentration and duration of exposure. The results showed that sterilizing agents 200 mg/L Cefotaxime and 100 mg/L Benomyl combined with NaOCl decreased the contamination of explants, and achieved 20% axenic culture.

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