scholarly journals 052 In Vitro Culture Initiation and Proliferation of Exochorda racemosa

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 397C-397
Author(s):  
Guochen Yang ◽  
Marihelen Kamp-Glass
Keyword(s):  
In Vitro Culture ◽  
Woody Plant ◽  
Shoot Proliferation ◽  
Deionized Water ◽  
In Vitro Cultures ◽  
Culture Initiation ◽  
Woody Plant Medium ◽  
Ornamental Shrub ◽  
Ornamental Species

Exochorda racemosa is an ornamental shrub with white flowers that is spiraea-like, deciduous, and hardy. The buds resemble pearls. Normally it is propagated by seeds, layers, and cuttings of softwood. However, it is a slow process that takes a few years to produce a reasonable size plant for the demanding market. Our objective was to establish a successful in vitro culture and to rapidly multiply this ornamental species. Softwood explant materials were collected from a local nursery and were disinfested with 15% bleach solution and rinsed three times with sterile distilled and deionized water. In vitro cultures were established and maintained in woody plant medium (WPM) supplemented with BA at 0.1 mg·L-1, 3% sucrose, and 0.7% agar with the pH adjusted to 5.8. Then shoots were transferred to the multiplication medium containing BA, CPPU, or thidiazuron (TDZ) at various concentrations. Preliminary results show that explants cultured on medium containing TDZ produced the best shoot proliferation.

Period of harvest, sprouting ability of cuttings, and in vitro plant regeneration in Fraxinus excelsior

1994 ◽  
Vol 72 (2) ◽  
pp. 261-267 ◽  
Author(s):  
Conceição Eneida Silveira ◽  
Alain Cottignies
Keyword(s):  
Chromosome Number ◽  
In Vitro Culture ◽  
Adventitious Roots ◽  
Woody Plant ◽  
Fraxinus Excelsior ◽  
Stem Cuttings ◽  
Natural Conditions ◽  
Apical Bud ◽  
Woody Plant Medium

Propagation by stem cuttings and in vitro culture of apical bud explants were studied on Fraxinus excelsior L. Stem cuttings from 4- to 7-year-old trees growing under natural conditions sprouted only when cuttings were taken from dormant material. Only 6% of those that had sprouted developed roots by the 7th month of culture. Similarly, only apical bud explants harvested during the dormant period sprouted in vitro. Up to 87% of these sprouts developed two to four branching adventitious roots after 5 months of culture. During the initial phase of in vitro culture, the Quoirin and Lepoivre medium and the woody plant medium favoured sprout lengthening. During the phase of multiplication, up to three sprouts per explant developed with the woody plant medium in the presence of a combination of high 6-benzylaminopurine (3.0–4.0 mg∙L−1) and low indole-3-butyric acid (0.01–0.03 mg∙L−1) concentrations. Rooting was obtained in a medium without any growth regulators. Microscopic analysis showed a direct connection between the vascular elements of adventitious roots and stem of plantlet. Chromosome number in root apices of ash plantlets and ash trees grown under natural conditions was 2n = 46. Key words: chromosome number, Fraxinus excelsior L., in vitro plants, micropropagation, stem cuttings.

scholarly journals MICROPROPAGATION OF ARONIA ARBUTIFOLIA AND A. MELANOCARPA

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1096a-1096
Author(s):  
Mark H. Brand ◽  
William G. Cullina
Keyword(s):  
Rapid Growth ◽  
Woody Plant ◽  
Shoot Proliferation ◽  
Breeding Programs ◽  
Murashige And Skoog Medium ◽  
Woody Plant Medium ◽  
Culture Morphology ◽  
Selection Of ◽  
Growing Shoot

Increasing interest in landscape use of Aronia arbutifolia and A. melanocarpa has led to the establishment of breeding programs and selection of improved phenotypes within the genus. A rnicropropagation system was developed to facilitate rapid and easy multiplication of improved forms of Aronia. Actively growing shoot tips of A. arbutifolia `Brilliantissima' and A. melanocarpa were used to initiate shoot proliferation from axillary buds. Optimum proliferation of shoots useful for micropropagation occurred on media supplemented with 0.5 to 1.0 mg 1-1 benzyladenine. Both Murashige and Skoog medium and Woody Plant medium supported vigorous shoot proliferation, but differences in culture morphology were evident. In vitro rooting and non-sterile rooting methods both resulted in high rooting percentages, the formation of numerous roots and subsequent rapid growth of plantlets.

scholarly journals Use of Forcing Solution Techniques to Improve Chestnut Micropropagation

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 757A-757 ◽  
Author(s):  
Guochen Yang ◽  
Paul E. Read ◽  
Marihelen Kamp-Glass
Keyword(s):  
Woody Plant ◽  
Shoot Proliferation ◽  
Contamination Rate ◽  
Regenerate Shoot ◽  
Normal Morphology ◽  
Woody Plant Medium ◽  
Forcing Solution ◽  
High Contamination Rate ◽  
Solution Techniques

Chestnut (Castanea spp.) is considered difficult to micropropagate. The timing for harvesting explant materials from forced stems is critical, although many factors need to be considered for successful micropropagation. Previous research with spirea and five-leaf aralia demonstrated that forcing solution techniques extended the availability of high-quality explant material, thus expediting micropropagation. However, preliminary research illustrated that chestnut is very difficult to force and the new forced softwood growth is very short-lived, which made micropropagation difficult. It was found that, at about 7 days from budbreak, the forced chestnut softwood growth (about 2 cm long) served as the best explant material. If longer than this timing window, the new growth would die. If shorter, the explants had a high contamination rate, exudation of purported phenolic compounds, and explants would not regenerate. Shoot proliferation and callus regeneration were achieved by culturing good-quality explants on Woody Plant Medium supplemented with 0.1 mg BA/liter. The new shoots grew vigorously in vitro with apparent normal morphology.

scholarly journals How Carbon Source and Seedcoat Influence the In Vitro Culture of Peach (Prunus persica L. Batsch) Immature Seeds

HortScience ◽  
2021 ◽  
pp. 1-2
Author(s):  
Margarita Pérez-Jiménez ◽  
Alfonso Guevara-Gázquez ◽  
Antonio Carrillo-Navarro ◽  
José Cos-Terrer
Keyword(s):  
Seed Germination ◽  
Carbon Source ◽  
In Vitro Culture ◽  
Seedling Growth ◽  
Woody Plant ◽  
Prunus Persica ◽  
In Vitro Germination ◽  
Immature Seeds ◽  
Woody Plant Medium

The effects of carbon source and concentration and of seedcoat were tested on the in vitro germination of peach seeds derived from crosses performed in the field. Seeds were extracted from the fruit and cultured in Woody Plant Medium (WPM) supplemented with sucrose, glucose, or sorbitol at concentrations of 15, 30, and 45 g·L−1. The percentage of germination as well as the root and hypocotyl lengths were measured after the stratification process and before acclimatization. Seedcoat did not have any influence on seed germination in any tested media and genotype. Glucose at a concentration of 15 g·L−1 and sucrose at 15, 30, and 45 g·L−1 resulted in greater stem seedling growth. The root developed the most when seeds were cultured in media with 15 or 30 g·L−1 of sucrose.

scholarly journals In vitro tissue culture initiation from potted and garden Hydrangea macrophylla explants

Agricultura ◽  
2016 ◽  
Vol 13 (1-2) ◽  
pp. 65-69
Author(s):  
Metka Šiško
Keyword(s):  
Survival Rate ◽  
Woody Plant ◽  
Contamination Rate ◽  
Multiplication Rate ◽  
Culture Initiation ◽  
Hydrangea Macrophylla ◽  
Genotype 2 ◽  
Woody Plant Medium ◽  
Dichloroisocyanuric Acid

Abstract The effect of sterilisation treatments on the initiation of the culture of three Hydrangea macrophylla genotypes in vitro was studied. The results indicated difference among different disinfection treatment; the consequence of treatments with dichloroisocyanuric acid, in combination with silver nitrate, was a significantly higher survival rate comparing to treatments based on NaOCl. Success of sterilisation was significantly influenced by studied genotypes. Two potted hydrangea genotypes (G2 and G3) showed higher survival rate (45.00 %, 55.00 %, respectively) comparing to the genotype 1 (originated from a local garden), which exhibited the highest percentage of contamination rate (58.75 %). Culturing the explants on a McCown woody plant medium supplemented with BAP at 2 mg/L and NAA at 0.005 mg/L produced the highest number of shoots with multiplication rate of 1.31 in 5 weeks. The highest rooting percentage (100.00 %) was obtained with the genotype 2 on a McCown woody plant medium supplemented with 0.5 mg/L IBA.

scholarly journals In Vitro and Macropropagation of the Wildflower Dyssodia pentacheta (D. C.) Robins

HortScience ◽  
1991 ◽  
Vol 26 (12) ◽  
pp. 1555-1557
Author(s):  
Thomas W. Zimmerman ◽  
Fred T. Davies ◽  
Jayne M. Zajicek
Keyword(s):  
Shoot Growth ◽  
Woody Plant ◽  
Shoot Proliferation ◽  
Nodal Segments ◽  
Naphthaleneacetic Acid ◽  
Stem Cuttings ◽  
Nodal Explant ◽  
System A ◽  
Woody Plant Medium

Dyssodia pentacheta, a prostrate-growing perennial Texas wildflower with potential for use in low-maintenance landscapes, was propagated in vitro and by stem cuttings under mist. Optimum rooting for IBA-treated semihardwood terminal stem cuttings (3 to 30 mm IBA) and in vitro-grown nodal segments (30 to 100 mm IBA) occurred after 4 weeks under an intermittent mist system. A 300-mm IBA basal dip was lethal to macroand microcuttings. In vitro, D. pentacheta produced more shoots per nodal explant on Woody Plant Medium (2 g Gelrite/liter) with 1 to 10 μ m BA than with combinations of BA and 0.5 μm NAA. After shoot proliferation, the shoots were subculture twice and grown on growth regulator-free medium. When maintaining D. pentacheta in vitro on media devoid of plant growth regulators, 1% sucrose was more effective than 2% for promoting shoot growth and suppressing apparent production of phenolics. Chemical names used: N-(phenylmethyl) -1H-purin-6-amine (BA); 1H-indole-3-butyric acid (IBA); 1-naphthaleneacetic acid (NAA).

scholarly journals Micropropagation of Betula platyphylla ‘Fargo’ via Shoot Tip Culture and Regeneration from Leaf Tissues

2000 ◽  
Vol 18 (2) ◽  
pp. 119-122 ◽  
Author(s):  
Zong-Ming Cheng ◽  
Jeffrey P. Schnurr ◽  
Wenhao Dai
Keyword(s):  
Woody Plant ◽  
Leaf Explants ◽  
Shoot Proliferation ◽  
Betula Platyphylla ◽  
Ex Vitro ◽  
White Birch ◽  
Dark Treatment ◽  
Woody Plant Medium ◽  
Leaf Tissues

Abstract A micropropagation system was developed for mass propagation of ‘Fargo’® (Dakota Pinnacle™), a newly released cultivar of Asian white birch (Betula platyphylla). Shoot tips from the mature, 7-year-old tree were established on 75% strength Murashige and Skoog medium supplemented with 0.1 μM thidiazuron. The greatest shoot proliferation occurred on Woody Plant Medium supplemented with 2.2 μM benzyladenine (BA), solidified with 6.5 g/liter agar, and cultured at 24C (75F). Microshoots were rooted in vitro or ex vitro followed by establishment in the greenhouse. A system to regenerate plantlets from leaves of aseptically cultured shoots was also developed. The optimum conditions for shoot regeneration from leaves included a 2-week dark treatment before exposure to a 16/8 hour light/dark photoperiod, use of large and healthy leaf explants, and culture on the Woody Plant Medium containing 20.0 or 30.0 μM BA. The regenerated shoots proliferated on the micropropagation medium and were divided, and the resulting shoots were rooted ex vitro and acclimated in greenhouse conditions.

scholarly journals In vitro establishment and proliferation of red currant cultivars

2012 ◽  
Vol 39 (No. 1) ◽  
pp. 21-25 ◽  
Author(s):  
J. Sedlák ◽  
F. Paprštein
Keyword(s):  
Woody Plant ◽  
Adventitious Bud ◽  
Mineral Salts ◽  
The Czech Republic ◽  
Shoot Number ◽  
Adventitious Bud Formation ◽  
Woody Plant Medium ◽  
In Vitro Establishment ◽  
Red Currant

The goal of this study was to investigate in vitro multiplication protocols for use with red currant cultivars grown in the Czech Republic. Cultivars Detvan, Vitan and Rotte Höllandische were successfully established in vitro using mercuric chloride in a concentration of 0.15% as a sterilization solution. The overall rate of contamination was 25.7%. Two proliferation media Murashige and Skoog medium (MS) and McCown woody plant medium (WPM) containing 1 or 2 mg/l of 6-benzylaminopurine (BAP) were tested. Initial explants produced new plants in the form of rosettes. Rosettes arose from the base of the initial explants in the form of adventitious bud formation. The shoot number was relatively low and varied between 1.0 and 2.1. Generally, the highest number was obtained for cultivar Rotte Holländische that produced 2.1 ± 0.1 new rosettes on MS medium containing lower concentration 1 mg/l BAP. In contrary, Vitan cv. had significantly lower shoot number ranging from 1.0 to 1.3. WPM medium with a lower concentration of mineral salts proved to be unsuitable for the multiplication of tested cultivars.

Manipulation of desiccation-sensitive axes of wampee (Clausena lansium) to facilitate increased dehydration tolerance

2000 ◽  
Vol 10 (3) ◽  
pp. 397-400
Author(s):  
J.R. Fu ◽  
X.M. Huang ◽  
S.Q. Songa
Keyword(s):  
Butyric Acid ◽  
Water Loss ◽  
Adventitious Roots ◽  
Woody Plant ◽  
Dehydration Tolerance ◽  
Woody Plant Medium ◽  
Napthaleneacetic Acid ◽  
Pre Treatment

AbstractThe plumules of newly-excised wampee embryos, which are more sensitive to dehydration than the roots, became more resistant to water loss when axes were allowed to sprout on woody plant medium [WPM; McCown and Lloyd (1981) Hortscience16, 453] before being dried. Pre-treatment of sprouting axes (seedlings) with sucrose incorporated in the WPM enhanced survival. Although the roots withered following further dehydration of seedlings cultured on WPM containing 60% sucrose, excised plumules were capable of generating adventitious roots when a combination of 10 mM α-napthaleneacetic acid and 10 mM indole-3-butyric acid was used during subsequent in vitro incubation.

scholarly journals Micropropagation of sea buckthorn (Hippophae rhamnoides L.)

1995 ◽  
Vol 4 (5-6) ◽  
pp. 503-512
Author(s):  
Yingmou Yao
Keyword(s):  
Growth Regulator ◽  
Woody Plant ◽  
Average Rate ◽  
Root Nodules ◽  
Optimal Concentration ◽  
Sea Buckthorn ◽  
Adventitious Buds ◽  
Woody Plant Medium ◽  
Different Origins

A protocol for micropropagation of sea buckthorn was developed starting with shoot tips or meristems from plants up to 18 years old. Among the different media used, the best medium for both initiation and multiplication was the woody plant medium (WPM). 6-Benzylaminopurine (BAP) was the most suitable growth regulator with an optimal concentration of 0.10-0.25 mg/l for initiation and 0.4-1.0 mg/l for multiplication. On WPM medium with BAP, the average rate of multiplication in Erlenmeyer flasks was 3.3-4.0 shoots per explant per month and in test tubes 2.0-3.0 shoots. Moreover, most explants produced several to tens of adventitious buds which grew into shoots. Explants rooted spontaneously in the multiplication medium at a frequency of about 33%. With this method, explants of different origins have been successfully propagated in vitro; and rooted young plants which had developed root nodules were produced both in the greenhouse and in the field.

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