scholarly journals PHILOGENETIC DIVERSITY OF FUNGI OF THE GENUS PENICILLIUM AND TALAROMYCES ASSOCIATED WITH BROWN ALGAE OF THE GENUS SARGASSUM

2020 ◽  
Vol 3 (9(78)) ◽  
pp. 12-18
Author(s):  
N. Kirichuk ◽  
M. Pivkin ◽  
Yu. Hudyakova
Keyword(s):  
Phylogenetic Analysis ◽  
Species Identification ◽  
Sea Of Japan ◽  
Brown Algae ◽  
Phylogenetic Diversity ◽  
Molecular Genetic ◽  
The Sea Of Japan ◽  
Gene Sequences ◽  
Penicillium Species ◽  
As Species

The results of the investigation of Penicillium sensu lato phylogenetic diversity of brown algae Sargassum spp. (the Sea of Japan) are presented in this article. Seventeen fungal strains were studied using traditional methods of phenotypic investigations and modern molecular-genetic approaches. As a result of phylogenetic analysis of ITS and BenA gene sequences, Penicillium species from five sections were revealed (sections Fasciculata, Paradoxa, Ramosa, Canescentia, Aspergilloides). One strain was identified as species of Talaromyces which more related to section Talaromyces. Five species (P. spinulosum, P. subspinulosum, P. roseomaculatum, P. thomii, P. murcianum, P. antarcticum) were identified based on BenA gene analysis. For species identification of some strains additional analysis of genetic and phenotypic features is necessary. 

scholarly journals Legume Symbiotic Nitrogen Fixation byβ-Proteobacteria Is Widespread inNature

2003 ◽  
Vol 185 (24) ◽  
pp. 7266-7272 ◽  
Author(s):  
Wen-Ming Chen ◽  
Lionel Moulin ◽  
Cyril Bontemps ◽  
Peter Vandamme ◽  
Gilles Béna ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Nitrogen Fixation ◽  
Phylogenetic Tree ◽  
Phylogenetic Diversity ◽  
Symbiotic Nitrogen Fixation ◽  
The Other ◽  
Gene Sequences ◽  
Free Living ◽  
Initial Discovery ◽  
Different Strains

ABSTRACT Following the initial discovery of two legume-nodulating Burkholderia strains (L. Moulin, A. Munive, B. Dreyfus, and C. Boivin-Masson, Nature 411:948-950, 2001), we identified as nitrogen-fixing legume symbionts at least 50 different strains of Burkholderia caribensis and Ralstonia taiwanensis, all belonging to the β-subclass of proteobacteria, thus extending the phylogenetic diversity of the rhizobia. R. taiwanensis was found to represent 93% of the Mimosa isolates in Taiwan, indicating thatβ -proteobacteria can be the specific symbionts of a legume. The nod genes of rhizobial β-proteobacteria (β-rhizobia) are very similar to those of rhizobia from theα -subclass (α-rhizobia), strongly supporting the hypothesis of the unique origin of common nod genes. Theβ -rhizobial nod genes are located on a 0.5-Mb plasmid, together with the nifH gene, in R. taiwanensis and Burkholderia phymatum. Phylogenetic analysis of available nodA gene sequences clustered β-rhizobial sequences in two nodA lineages intertwined with α-rhizobial sequences. On the other hand, theβ -rhizobia were grouped with free-living nitrogen-fixingβ -proteobacteria on the basis of the nifH phylogenetic tree. These findings suggest that β-rhizobia evolved from diazotrophs through multiple lateral nod gene transfers.

The current approaches to the study of algae: DNA barcoding and DNA taxonomy

2021 ◽  
pp. 124-130
Author(s):  
Anna D. Temraleeva ◽  
Elena S. Krivina ◽  
Yury S. Bukin
Keyword(s):  
Phylogenetic Analysis ◽  
Dna Sequencing ◽  
Dna Barcoding ◽  
Species Identification ◽  
Green Algae ◽  
Molecular Genetic ◽  
Algal Species ◽  
Species Boundaries ◽  
Sequencing Technology ◽  
Dna Taxonomy

The understanding of the impossibility of distinguishing algal species based on morphological features came with the development of DNA sequencing technology, which today is a necessary tool for defining species boundaries and testing traditional species concepts. The paper discusses popular approaches to species identification (DNA barcoding) and the description of new and revision of known species (DNA taxonomy) using molecular genetic methods. The requirements and limitations in their work are given, as well as examples of phylogenetic analysis of green algae from the clade Moewusinia and Parachlorella, including the genus Micractinium.

Phylogenetic study and identification of human pathogenicVibriospecies based on partialhsp60 gene sequences

2002 ◽  
Vol 48 (10) ◽  
pp. 903-910 ◽  
Author(s):  
Anita Y.C Kwok ◽  
Jason T Wilson ◽  
Michael Coulthart ◽  
Lai-King Ng ◽  
Lucy Mutharia ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
16S Rrna ◽  
Species Identification ◽  
Vibrio Species ◽  
Phylogenetic Study ◽  
Rrna Gene ◽  
Gene Sequences ◽  
Hsp60 Gene ◽  
Sequence Identity ◽  
Marine Vibrios

The use of hsp60 gene sequences for phylogenetic study and identification of pathogenic marine vibrios was investigated. A 600-bp partial hsp60 gene was amplified by PCR and sequenced from 29 strains representing 15 Vibrio species within the family Vibrionaceae. Sequence comparison of the amplified partial hsp60 gene revealed 71–82% sequence identity among different Vibrio species and 96–100% sequence identity among epidemiologically distinct strains with the same species designation. This degree of discrimination allows unambiguous differentiation of all Vibrio species included in the current study from each other, as well as from Aeromonas hydrophila and Plesiomonas shigelloides, which are often misidentified as Vibrio species by conventional biochemical methods. Based on the hsp60 gene sequences, two previously unidentified shrimp isolates were found to be more closely related to Vibrio alginolyticus (93–94% sequence identity) than to Vibrio parahaemolyticus (89% sequence identity), whereas 16S rRNA gene analysis was unable to differentiate among these closely related species (95–97% sequence identity). Our results indicate that the hsp60 gene may be a useful alternative target for phylogenetic analysis and species identification of marine Vibrios to complement more conventional identification systems.Key words: Vibrio, hsp60, 16S rRNA, phylogenetic analysis, species identification.

Application of PCR-RFLP technique to species identification and phylogenetic analysis of Streptomyces associated with potato scab in Brazil based on partial atpD gene sequences

2014 ◽  
Vol 142 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Daniele Bussioli Alves Corrêa ◽  
Denise Salomão ◽  
Júlio Rodrigues-Neto ◽  
Ricardo Harakava ◽  
Suzete Aparecida Lanza Destéfano
Keyword(s):  
Phylogenetic Analysis ◽  
Species Identification ◽  
Potato Scab ◽  
Gene Sequences ◽  
Pcr Rflp

scholarly journals Differentiation of the frog sculpin Myoxocephalus stelleri Tilesius, 1811 (Actinopterygii, Cottidae) based on mtDNA and karyotype analyses

2021 ◽  
Vol 15 (2) ◽  
pp. 179-197
Author(s):  
Irina N. Moreva ◽  
Olga A. Radchenko ◽  
Anna V. Petrovskaya
Keyword(s):  
Sea Of Japan ◽  
Coastal Waters ◽  
Sea Of Okhotsk ◽  
Molecular Genetic ◽  
Kuril Islands ◽  
The Sea Of Japan ◽  
Large Area ◽  
Phylogenetic Groups ◽  
The Sea Of Okhotsk ◽  
Shikotan Island

A molecular genetic and karyological study of the frog sculpin Myoxocephalus stelleri Tilesius, 1811 was carried out on an extensive sample from a large area of the species’ range. A total of 42 specimens was sampled from the Sea of Japan, Sea of Okhotsk, and coastal waters off the southern Kuril Islands, which makes this sampling scheme the most comprehensive to date. The level of mtDNA polymorphism was found to be low. The haplotypes of the species formed three phylogenetic groups. The unique M. stelleri haplotype from the coast of Shikotan Island linked all the studied groups, indicating that it is likely ancestral. Robertsonian polymorphism was identified in the species. In all five cytotypes (I – 2n = 44, II – 2n = 43, III – 2n = 42, IV – 2n = 41, V – 2n = 40; NF = 44+2) were identified, all of which were present in the Sea of Japan. Only one (cytotype I) was found in the Sea of Okhotsk, which is probably the closest to the ancestral karyotype. The significant chromosomal polymorphism and the presence of common haplotypes in the studied samples indicate their recent origin from a common ancestor and/or relatively recent contacts within the range. The discrepancies between mtDNA and karyotypes in assigning the ancestral M. stelleri to the coastal waters off Shikotan Island (southern Kuril Islands) and the Sea of Okhotsk, respectively, can be explained by the different inheritance mechanisms and the rates of evolution of molecular genetic and karyological traits.

Characteristics of Upward Leaders of Winter Lightning in the Coastal Area of the Sea of Japan

2012 ◽  
Vol 132 (6) ◽  
pp. 560-567 ◽  
Author(s):  
Megumu Miki ◽  
Toru Miki ◽  
Akira Asakawa ◽  
Takatoshi Shindo ◽  
Shigeru Yokoyama
Keyword(s):  
Sea Of Japan ◽  
Coastal Area ◽  
The Sea Of Japan
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