scholarly journals Effects of Electro-Acupuncture and Acupotomy Dissolution on mRNA Expression of Center Pro-Opiomelanocortin Detected by In Situ Hybridization in Rats with Non-Specific Low Back Pain

2016 ◽  
Vol 5 (4) ◽  
pp. 194-99
Author(s):  
Shapour Badiee Aval ◽  
Ali Khorsand ◽  
Seyed Javad Mojtabavi ◽  
Shima Rezaei Deloei ◽  
Guo Chang Qin
Keyword(s):  
Low Back Pain ◽  
Back Pain ◽  
Mrna Expression ◽  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Low Back ◽  
Model Group ◽  
Peripheral Tissues ◽  
Spinal Dorsal ◽  
Pomc Mrna

Background: Pro-opiomelanocortin (POMC) mainly exists in the pituitary gland, hypothalamus, and peripheral tissues and can relieve pain through its degradation product β-endorphin. Its mRNA expression quantity represents the level of gene expression of endorphin system. We aimed to determine the effects of electro-acupuncture and acupotomy dissolution on the mRNA expression of center POMC in rats with non-specific low back pain.Materials and Methods: This study was performed on 42 Sprague-Dawley rats in four groups of normal, model, electro-acupuncture, and acupotomy. The normal group did not receive any intervention, while non-specific low back pain was established in other groups. Then, the model group did not receive any treatment, electro-acupuncture and acupotomy groups were treated with electro-acupuncture therapy and acupotomy, respectively. Microscopic images of the slices, prepared from spinal dorsal horn and hypothalamus, were analyzed to evaluate the mRNA expression of center POMC. Results: Under light microscopy examination, the positive POMC mRNA cells of electro-acupuncture and acupotomy groups increased more than the model group, while its expression in the hypothalamus and spinal dorsal horn was less than the model group, but the difference was not significant (P<0.01). Conclusion: Electro-acupuncture and acupotomy could reduce POMC mRNA expression in spinal cord and increase it in the hypothalamus of rats with non-specific low back pain.[GMJ.2016;5(4):194-99]

scholarly journals A Role for Global DNA Methylation Level and IL2 Expression in the Transition From Acute to Chronic Low Back Pain

2021 ◽  
Vol 2 ◽  
Author(s):  
Olivia C. Eller ◽  
Nicole Glidden ◽  
Brittany Knight ◽  
Noelle McKearney ◽  
Mallory Perry ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Low Back Pain ◽  
Back Pain ◽  
Histone Acetylation ◽  
Mrna Expression ◽  
Interleukin 2 ◽  
Global Dna Methylation ◽  
Low Back ◽  
Recent Onset ◽  
Acute Low Back Pain

Objectives: The transition from acute low back pain (aLBP) to chronic LBP (cLBP) results from a variety of factors, including epigenetic modifications of DNA. The aim of this study was to (1) compare global DNA (gDNA) methylation and histone acetylation at LBP onset between the aLBP and cLBP participants, (2) compare mRNA expression of genes with known roles in the transduction, maintenance, and/or modulation of pain between the aLBP and cLBP participants, (3) compare somatosensory function and pain ratings in our participants, and (4) determine if the aforementioned measurements were associated.Methods: A total of 220 participants were recruited for this prospective observational study following recent onset of an episode of LBP. We retained 45 individuals whose gDNA was of sufficient quality for analysis. The final sample included 14 participants whose pain resolved within 6 weeks of onset (aLBP),15 participants that reported pain for 6 months (cLBP), and 16 healthy controls. Participants were subjected to quantitative sensory testing (QST), blood was drawn via venipuncture, gDNA isolated, and global DNA methylation and histone acetylation, as well as mRNA expression of 84 candidate genes, were measured.Results: Individuals that develop cLBP display multimodal somatosensory hypersensitivity relative to aLBP participants. cLBP participants also had significantly lower global DNA methylation, which was negatively correlated with interleukin-2 (IL2) mRNA expression.Discussion: cLBP is characterized by somatosensory hypersensitivity, lower global DNA methylation, and higher IL2 expression level compared to those whose pain will resolve quickly (aLBP). These results suggest potential diagnostic and therapeutic relevance for global DNA methylation and IL2 expression in the pathology underlying the transition from acute to chronic LBP.

510 Effect of inhalational anesthetics on dynorphin mRNA expression in spinal dorsal horn neurons after noxious stimuli

1997 ◽  
Vol 28 ◽  
pp. S80
Author(s):  
Masaaki Tanimoto ◽  
Chikara Tashiro ◽  
Atsushi Tokunaga ◽  
Kenji Miki ◽  
Tetsuo Fukuoka ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Dorsal Horn Neurons ◽  
Spinal Dorsal ◽  
Inhalational Anesthetics ◽  
Spinal Dorsal Horn Neurons ◽  
Noxious Stimuli

scholarly journals Prevention and reversal of latent sensitization of dorsal horn neurons by glial blockers in a model of low back pain in male rats

2017 ◽  
Vol 118 (4) ◽  
pp. 2059-2069 ◽  
Author(s):  
Juanjuan Zhang ◽  
Siegfried Mense ◽  
Rolf-Detlef Treede ◽  
Ulrich Hoheisel
Keyword(s):  
Low Back Pain ◽  
Back Pain ◽  
Nerve Growth Factor ◽  
Dorsal Horn ◽  
Microglial Activation ◽  
Low Back ◽  
Astrocyte Activation ◽  
Dorsal Horn Neurons ◽  
Resting Activity ◽  
Stimulation Of

In an animal model of nonspecific low back pain, recordings from dorsal horn neurons were made to investigate the influence of glial cells in the central sensitization process. To induce a latent sensitization of the neurons, nerve growth factor (NGF) was injected into the multifidus muscle; the manifest sensitization to a second NGF injection 5 days later was used as a read-out. The sensitization manifested in increased resting activity and in an increased proportion of neurons responding to stimulation of deep somatic tissues. To block microglial activation, minocycline was continuously administered intrathecally starting 1 day before or 2 days after the first NGF injection. The glia inhibitor fluorocitrate that also blocks astrocyte activation was administrated 2 days after the first injection. Minocycline applied before the first NGF injection reduced the manifest sensitization after the second NGF injection to control values. The proportion of neurons responsive to stimulation of deep tissues was reduced from 50% to 17.7% ( P < 0.01). No significant changes occurred when minocycline was applied after the first injection. In contrast, fluorocitrate administrated after the first NGF injection reduced significantly the proportion of neurons with deep input (15.8%, P < 0.01). A block of glia activation had no significant effect on the increased resting activity. The data suggest that blocking microglial activation prevented the NGF-induced latent spinal sensitization, whereas blocking astrocyte activation reversed it. The induction of spinal neuronal sensitization in this pain model appears to depend on microglia activation, whereas its maintenance is regulated by activated astrocytes. NEW & NOTEWORTHY Activated microglia and astrocytes mediate the latent sensitization induced by nerve growth factor in dorsal horn neurons that receive input from deep tissues of the low back. These processes may contribute to nonspecific low back pain.

scholarly journals The Lumbodorsal Fascia as a Potential Source of Low Back Pain: A Narrative Review

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Jan Wilke ◽  
Robert Schleip ◽  
Werner Klingler ◽  
Carla Stecco
Keyword(s):  
Low Back Pain ◽  
Back Pain ◽  
Dorsal Horn ◽  
Morphological Changes ◽  
Low Back ◽  
Dorsal Horn Neurons ◽  
Potential Source ◽  
Stimulation Of

The lumbodorsal fascia (LF) has been proposed to represent a possible source of idiopathic low back pain. In fact, histological studies have demonstrated the presence of nociceptive free nerve endings within the LF, which, furthermore, appear to exhibit morphological changes in patients with chronic low back pain. However, it is unclear how these characteristics relate to the aetiology of the pain. In vivo elicitation of back pain via experimental stimulation of the LF suggests that dorsal horn neurons react by increasing their excitability. Such sensitization of fascia-related dorsal horn neurons, in turn, could be related to microinjuries and/or inflammation in the LF. Despite available data point towards a significant role of the LF in low back pain, further studies are needed to better understand the involved neurophysiological dynamics.

Short-term swimming exercise attenuates the sensitization of dorsal horn neurons in rats with NGF-induced low back pain

2018 ◽  
Vol 22 (8) ◽  
pp. 1409-1418 ◽  
Author(s):  
G. de Azambuja ◽  
U. Hortscht ◽  
U. Hoheisel ◽  
M.C. Oliveira Fusaro ◽  
S. Mense ◽  
...  
Keyword(s):  
Low Back Pain ◽  
Back Pain ◽  
Dorsal Horn ◽  
Swimming Exercise ◽  
Low Back ◽  
Short Term ◽  
Dorsal Horn Neurons
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