scholarly journals Progesterone supplementation to lactating dairy cows without a corpus luteum at initiation of the Ovsynch protocol

2015 ◽  
Vol 98 (4) ◽  
pp. 2515-2528 ◽  
Author(s):  
R.S. Bisinotto ◽  
L.O. Castro ◽  
M.B. Pansani ◽  
C.D. Narciso ◽  
N. Martinez ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Corpus Luteum ◽  
Lactating Dairy Cows ◽  
Progesterone Supplementation

Effect of progesterone supplementation on fertility responses of lactating dairy cows with corpus luteum at the initiation of the Ovsynch protocol

2015 ◽  
Vol 83 (2) ◽  
pp. 257-265 ◽  
Author(s):  
R.S. Bisinotto ◽  
M.B. Pansani ◽  
L.O. Castro ◽  
C.D. Narciso ◽  
L.D.P. Sinedino ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Corpus Luteum ◽  
Lactating Dairy Cows ◽  
Progesterone Supplementation

scholarly journals 141 Effect of induction of an ipsilateral vs. contralateral accessory corpus luteum (CL) on pregnancy per AI and pregnancy loss in lactating dairy cows

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 113-114
Author(s):  
Pedro L J Monteiro ◽  
Caio A Gamarra ◽  
Rodrigo S Genari ◽  
Alexandre B Prata ◽  
Rafael V Barletta ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Corpus Luteum ◽  
Pregnancy Loss ◽  
Statistical Analyses ◽  
Holstein Cows ◽  
Crown Rump Length ◽  
Blood Samples ◽  
Vesicle Size ◽  
Lactating Dairy Cows ◽  
In Pregnancy

Abstract Induction of accessory CL can increase circulating progesterone (P4) and potentially improve fertility; although, regression of accessory CL contralateral to the pregnancy can occur, potentially negating their benefit. In this study, primiparous (n = 377) and multiparous (n = 678) lactating Holstein cows (80.0 ± 3.3DIM, 43.2 ± 13.3 kg milk/d, and BCS = 2.85 ± 0.24) were enrolled in Presynch-Ovsynch (PGF-14d-PGF-12d-GnRH-7d-PGF-56h-GnRH-16h-AI) with AI at 81 ± 3 DIM. On d5 after AI, cows were randomly but unequally assigned as Control (n = 289) or GnRH (n = 641; 100 µg gonadorelin acetate). Blood samples were collected for P4 and ovaries evaluated by ultrasound on d5, d12, d19, d26, d33, d47, and d61. mRNA for ISGs (d19) and PSPB (d26) concentrations were evaluated and pregnancy diagnoses were done on d26, d33, d47 (also embryonic measurements), and d61. Statistical analyses were performed with PROC GLIMMIX of SAS 9.4. Ovulation to GnRH on d5 was 85.4% (577/676). Cows were designated as: Control (n = 289), Ipsilateral (n = 239), or Contralateral (n = 241). Overall P4 differed (P < 0.01) within groups (Control = 7.90 ± 0.35c, Ipsilateral = 10.5 ± 0.34a, and Contralateral = 9.55 ± 0.26a). Interestingly, 52.7% (78/148) of contralateral pregnant cows had accessory CL regression by d61 with decreases in P4 after contralateral CL regression (P < 0.001). There were no treatment differences for pregnant cows in ISG15 (P = 0.63) or Mx2 (P = 0.51) mRNA, circulating PSPB (P = 0.56), amniotic vesicle size (P = 0.89), or crown-rump length (P = 0.19) or in pregnancy/AI on d26 (P = 0.24), d33 (P = 0.67), d47 (P = 0.53), or d61 (P = 0.62; overall 50.3% [387/769]). Nevertheless, pregnancy losses between d26 and d61 were lower (P = 0.03) for ipsilateral (6.6 ± 2.3%) than contralateral (14.0 ± 3.0%) and from controls (P = 0.065; 13.7 ± 2.9%) With differences also from d26-33 (Control = 7.5 ± 2.1; Ipsilateral = 3.1 ± 1.5; Contralateral = 9.6 ± 2.5). Within contralateral group, early accessory CL regression was associated with greater pregnancy loss from d26-33 (P = 0.04) and d26-61 (P = 0.01). Thus, induction of accessory CL increases P4 and may reduce pregnancy loss, although these advantages are reduced for contralateral accessory CL, because many of these CL regress during pregnancy.

72 EFFECT OF PROGESTERONE SUPPLEMENTATION ON DAY 4 AFTER TIMED ARTIFICIAL INSEMINATION ON PREGNANCY RATE OF LACTATING DAIRY COWS

2017 ◽  
Vol 29 (1) ◽  
pp. 143
Author(s):  
G. Tortorelli ◽  
A. J. Azrak ◽  
V. da Costa Andrade ◽  
R. dos Santos Ramos ◽  
A. S. Moraes ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Pregnancy Rate ◽  
Fixed Time ◽  
Lactating Dairy Cows ◽  
Embryonic Loss ◽  
Progesterone Supplementation ◽  
Group 2 ◽  
Intravaginal Device ◽  
One Way Anova ◽  
Group 1

Progesterone plays an important role in the maintenance of pregnancy. It is hypothesised that insufficient progesterone early in pregnancy may result in embryonic loss, and that supplemental progesterone would decrease pregnancy loss in dairy cows. The aim of this study was to compare the effect of progesterone supplementation after timed AI on pregnancy rate of dairy cows. The experiment was conducted in a single commercial herd, in Sao Paulo State (Brazil), during a period of 1 year (January–December 2016). The cows were assigned at a nonpregnancy diagnosis 32 ± 3 days after AI to 1 to 6 resynchronization treatments. They were submitted to the same fixed time AI protocol: Day 0, 0.1 mg of gonadorelin, 2 mg of oestradiol benzoate, and an intravaginal device of 1.9 g of progesterone; Day 7, 25 mg of dinoprost tromethamine; Day 8, 25 mg of dinoprost tromethamine, 1 mg of oestradiol cipionate, and device withdrawal; Day 10, AI. At Day 14, the cows were alternately arranged in 2 groups. Group 1 received a 900-mg progesterone supplementation IM injection, and Group 2 did not receive progesterone supplementation. Then, 32 ± 3 days after insemination, pregnancy diagnosis was performed by transrectal ultrasonography, and the data were tabulated. Full statistical analysis will be done at the end of the study, when more than 500 cows will be allocated to the 2 groups. From January until April 2016, 230 lactating dairy cows were submitted to the experiment. Both groups had similar average days in lactation (166), average number of lactations (1.95), and average number of inseminations (4.3). Group 1 had a pregnancy rate of 25% (29/116), and group 2 had a pregnancy rate of 15.8% (18/114) (P = 0.01, one-way ANOVA). Based on these preliminary data, pregnancy rates of lactating dairy cows may be improved with progesterone supplementation on Day 4 after insemination. These results need to be confirmed on larger numbers of cows.

Follicular dynamics, corpus luteum growth and regression in lactating dairy cattle

1991 ◽  
Vol 71 (1) ◽  
pp. 61-68 ◽  
Author(s):  
C. Taylor ◽  
R. Rajamahendran
Keyword(s):  
Dairy Cows ◽  
Corpus Luteum ◽  
Estrous Cycle ◽  
Follicular Growth ◽  
Dominant Follicle ◽  
Lactating Dairy Cows ◽  
Ovulatory Follicle ◽  
Second Wave ◽  
Lactating Dairy Cattle ◽  
Follicular Dynamics

Ultrasound imaging and progesterone (P4) profiles were used to characterize follicular and corpus luteum (CL) growth and regression in 10 cycling dairy cows. Thirteen of sixteen cycles were characterized by two waves of follicular growth, the second wave giving rise to the ovulatory follicle. The remaining three cycles had three waves. There was no difference between two- and three-wave cycles with regard to the day of emergence of the first or second dominant follicle (day 2.5 ± 0.9 vs. day 2.4 ± 1.2 and day 12.4 ± 1.6 vs. day 12.0 ± 2.0, respectively) or the onset of regression of the first dominant follicle (day 12.5 ± 1.5 vs. day 12.3 ± 1.5). In two wave cycles, estrous cycle length was shorter (20.8 ± 1.7 d vs. 29.7 ± 4.9 d), the first day of decline in P4 (day 16.2 ± 1.5 vs. day 23.7 ± 5.5) and the onset of CL regression (day 17.1 ± 1.3 vs. day 24.3 ± 5.5) was earlier. It is concluded that two waves of follicular growth in an estrous cycle is the norm in lactating dairy cows. The presence of an additional wave is due to a prolonged luteal phase. Key words: Follicle, corpus luteum, estrous cycle, dairy cow

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