The measurement of muzzle vibration responses during gun firing is one of the most important factors for successful design of gun. The use and result are limited by traditional method for the effect of firing environment, so new measurement method is required, the high-speed imaging technology is one choice. The application of high-speed imaging technology in measurement of muzzle vibration is focused on qualitative analysis, in order to discuss the quantitative analysis in this paper, images of muzzle vibration responses are obtained by using high-speed vidicon. Image pretreatment is done for post processing expediently, the process of pretreatment include de-noising by using wavelet transform method, gray processing by using averaged-value method and contrast improvement by using wavelet transform method. After image pretreatment, orientation and matching is done by using correlative matching method, and then the numerical of muzzle vibration responses during gun firing are obtained. So a novel method is afforded for measuring of muzzle vibration responses during gun firing, and the new method has practical significance in the gun’s design, research and production.