scholarly journals Cytogenetic studies of three cultivated hill cotton (Gossypium arboreum L.) varieties from Bangladesh

2021 ◽  
Vol 81 (01) ◽  
pp. 135-138
Author(s):  
Afsana Hossain ◽  
Chandan Kumar Dash ◽  
Syeda Sharmeen Sultana
Keyword(s):  
Somatic Chromosome ◽  
Repetitive Sequences ◽  
Differential Staining ◽  
Gossypium Arboreum ◽  
Somatic Chromosome Number ◽  
Chromomycin A3 ◽  
Banding Patterns ◽  
Cytogenetic Studies ◽  
Karyotypic Diversity ◽  
Dapi Banding

Three hill cotton (Gossypium arboreum L.) varieties viz., HC-1, HC-2 and HC-3, released by Bangladesh Cotton Development Board were investigated through orcein, CMAand DAPI-banding for cytogenetical characterization and to elucidate the karyotypic diversity among these varieties. All these three varieties were found to possess 2n = 26 metacentric chromosomes with ‘1A’ karyotype. Based on TF%, AsK% and Syi index, HC-3 was little advanced over HC-1 and HC-2. These three varieties showed differential Chromomycin A3 (CMA)- and 4Ê-6 Diamidino-2-Phenyl Indole (DAPI)-banding patterns and a tendency of acumulation of repetitive sequences at the terminal regions was observed. Despite possessing same somatic chromosome number these three hill cotton varieties could be characterized by diversified karyotypic parameters through differential staining.

scholarly journals FIRST KARYOTYPE REPORT ON Colocasia oresbia: A COMPARATIVE CYTOGENETIC STUDY BETWEEN TWO VARIETIES

2020 ◽  
Vol 31 (Issue 2) ◽  
pp. 7-11
Author(s):  
N. Begum Kazi ◽  
K. Dash Chandan ◽  
S. Sultana Syeda
Keyword(s):  
Cytogenetic Study ◽  
Chromosome Length ◽  
Future Research ◽  
Somatic Chromosome Number ◽  
Chromomycin A3 ◽  
Banding Patterns ◽  
Ecological Zones ◽  
Karyotypic Formula ◽  
Dapi Banding

Karyotypes of two Colocasia oresbia botanical varieties from Bangladesh were analyzed and compared with orcein, chromomycin A3 (CMA) and 4´-6 diamidino-2-phenylindole (DAPI). Both varieties had 2n=2x=26 chromosomes (karyotypic formula: 20m+6sm) and a pair of satellites each. Total chromosome length was 144.18±2.45 μm in C. oresbia var. oresbia and 133.02±2.75 μm in C. oresbia var. stolonifera. The karyotype of Colocasia oresbia var. oresbia is 2A whereas that of C. oresbia var. stolonifera is 1A. Six CMA and four DAPI bands were observed in C. oresbia var. oresbia and eight CMA and six DAPI bands in C. oresbia var. stolonifera. However, in these two morphologically distinct C. oresbia varieties of two different ecological zones, the same somatic chromosome number, diversification in various karyotypic parameters and CMA/DAPI-banding patterns were observed. In addition to taxonomic characters, the studied karyotype features will contribute to the characterization of these two C. oresbia varieties and to establish a base for future research. Key words: chromosome banding; CMA; DAPI; Karyotype.

Cytogenetical analysis of Clitoria ternatea

2021 ◽  
Vol 8 (1) ◽  
pp. 71-77
Author(s):  
P. Phukan ◽  
M. A. Laskar ◽  
B. J. Mylliemngap ◽  
J. M. Lamo
Keyword(s):  
Somatic Chromosome ◽  
Heterochromatic Region ◽  
Somatic Chromosome Number ◽  
Chromomycin A3 ◽  
Important Species ◽  
Clitoria Ternatea ◽  
Nucleolar Organizing Region ◽  
The Family ◽  
Cytogenetical Analysis

Clitoria ternatea L. of the family Fabaceae is an economical, ornamental as well as medicinal important species. Chromosome characterization of C. ternatea, encompassing karyomorphological as well as fluorochrome binding was carried out in the present investigation. Karyomorphological studies showed the presence of 2n = 16 somatic chromosome number with three pairs of metacentric chromosomes and five pairs of submedian chromosomes. The analysis also revealed the present of one pair of nucleolar organizing region or satellite. Fluorochrome binding using chromomycin A3 (CMA) and 4-6-diamidino-2-phenylindole (DAPI) showed the presence of GC- and AT- rich heterochromatic region. Further analysis revealed the percentage of GC-rich was comparatively higher than AT-rich heterochromatic region. This is the first report on heterochromatin characterization in C. ternatea.

CYTOGENETIC STUDIES IN AMARANTHUS: I. CYTOLOGICAL ASPECTS OF SEX DETERMINATION IN DIOECIOUS SPECIES

1959 ◽  
Vol 37 (3) ◽  
pp. 413-417 ◽  
Author(s):  
William F. Grant
Keyword(s):  
Chromosome Number ◽  
Sex Determination ◽  
Chromosome Numbers ◽  
Somatic Chromosome ◽  
Pollen Grain ◽  
Somatic Chromosome Number ◽  
Dioecious Species ◽  
Absolute Size ◽  
Morphological Studies ◽  
Cytogenetic Studies

Cytological observations are contained in this report on four dioecious species of Amaranthns, two of which were formerly included in the genus Acnida. The species studied are A. arenicola, A. palmeri, A. tamariscinus, and A. tuberculatus. Three of these species have a diploid chromosome number of 32, whereas A. palmeri has a somatic chromosome number of 34. A single spontaneous triploid (2n = 48) female plant was found in collections of A. tamariscinus and a tetraploid (2n = 64) male plant in collections of A. tuberculatus. A fifth species, A. australis, has previously been reported as having 32 somatic chromosomes. The chromosome numbers for half the dioecious species of Amaranthus have now been determined. Detailed observations on the dividing chromosomes of these species in mitosis, in meiosis, and in the first division of the nucleus in the pollen grain have been made and have failed to distinguish heteromorphic chromosomes which might be associated with sex determination. The small size of the chromosomes has made detailed morphological studies impractical and there is no marked difference in absolute size of the chromosomes between species. Since haploid numbers of 16 and 17 are found in both monoecious and dioecious species, it would seem that the aneuploid condition in Amaranthus arose early and hybridization within the genus has resulted in promoting the genie condition which has been necessary for the expression of the dioecious condition.

Chromosome morphology of Clematis, subsection Viornae (Ranunculaceae)

1976 ◽  
Vol 54 (10) ◽  
pp. 1135-1139 ◽  
Author(s):  
W. Michael Dennis
Keyword(s):  
Chromosome Number ◽  
Somatic Chromosome ◽  
Chromosome Complement ◽  
Chromosome Morphology ◽  
Terminal Region ◽  
Somatic Chromosome Number ◽  
Median Region ◽  
Acrocentric Chromosomes ◽  
Subterminal Region ◽  
Marked Stability

Cytological studies were made on the following taxa: C. addisonii, C. filifera, C. glaucophylla, C. pitcheri, C. reticulata, C. texensis, C. versicolor, and C. viorna. All species were found to have a somatic chromosome number of 16 with a uniform karyotype consisting of five pairs of metacentric chromosomes with centromeres in the median region and three pairs of acrocentric chromosomes, two pairs with centromeres in the terminal region and one pair with centromeres in the subterminal region. These findings agree with reports of chromosome number and karyotype for other species of Clematis and suggest a marked stability of chromosome complement in the genus.

Investigations of the inheritance of flower variegation in Mirabilis jalapa L. 3. Somatic chromosome number, 4. Distribution of the pigments and 5. Chromatographic studies

Euphytica ◽  
1975 ◽  
Vol 24 (1) ◽  
pp. 6-12 ◽  
Author(s):  
W. N. M. Van Kester ◽  
C. J. T. Spitters ◽  
L. Vosselman ◽  
J. M. M. Engels ◽  
A. C. Zeven
Keyword(s):  
Chromosome Number ◽  
Somatic Chromosome ◽  
Somatic Chromosome Number ◽  
Mirabilis Jalapa

Allium kyrenium (Amaryllidaceae), a new species from Northern Cyprus

Phytotaxa ◽  
2015 ◽  
Vol 213 (3) ◽  
pp. 282 ◽  
Author(s):  
GIANPIETRO GIUSSO DEL GALDO ◽  
CRISTIAN BRULLO ◽  
Salvatore Brullo ◽  
CRISTINA SALMERI
Keyword(s):  
New Species ◽  
Somatic Chromosome ◽  
Eastern Mediterranean ◽  
Conservation Status ◽  
Diploid Species ◽  
Mediterranean Area ◽  
Somatic Chromosome Number ◽  
Northern Cyprus ◽  
Eastern Mediterranean Area ◽  
A New Species

Allium kyrenium, a new species of Allium sect. Codonoprasum, is described and illustrated from northern Cyprus. It is a very circumscribed geophyte growing on the calcareous cliffs of the Kyrenia range. This diploid species, with a somatic chromosome number 2n = 16, shows close morphological relationships with A. stamineum, a species complex distributed in the eastern Mediterranean area. Its morphology, karyology, leaf anatomy, ecology, conservation status and taxonomical relationships with the allied species belonging to the A. stamineum group are examined.

Fluorescent banding patterns of the chromosomes of the genus Salmo

Genome ◽  
1988 ◽  
Vol 30 (2) ◽  
pp. 193-197 ◽  
Author(s):  
Ruth B. Phillips ◽  
Sheila E. Hartley
Keyword(s):  
Great Britain ◽  
North America ◽  
Acrocentric Chromosome ◽  
Organizer Region ◽  
Nucleolar Organizer Region ◽  
Nucleolar Organizer ◽  
Salmo Gairdneri ◽  
Chromomycin A3 ◽  
Banding Patterns ◽  
Fluorescent Banding

The fluorescent banding patterns of the chromosomes of Salmo gairdneri (rainbow trout), Salmo trutta (brown trout), and Salmo salar (Atlantic salmon) from North America and Great Britain are described. Quinacrine stained a subset of C bands in S. gairdneri and S. salar from North America. No bright quinacrine (Q) bands were found on the chromosomes of S. salar from Great Britain or the chromosomes from any of the three stocks of S. trutta that were examined. Q bands were found at the centromeres of three to seven different chromosome pairs in S. gairdneri, including the pair that has been identified as the sex chromosome pair in some populations. In S. salar from North America the Q bands were found at the teleomeres of three to four chromosome pairs and at interstitial locations in the 10–13 large acrocentric chromosome pairs. Chromomycin A3 stained either the nucleolar organizer region or the adjacent heterochromatin or both in all three species. In S. trutta the entire short arm of the acrocentric chromosome containing the nucleolar organizer region always stained with chromomycin A3 while in S. gairdneri and S. salar the staining properties of the NOR and adjacent heterochromatin were polymorphic.Key words: banding, C-, Q-; Salmo; trout.

scholarly journals Chromosome Banding in Amphibia. XXXII. The Genus Xenopus (Anura, Pipidae)

2015 ◽  
Vol 145 (3-4) ◽  
pp. 201-217 ◽  
Author(s):  
Michael Schmid ◽  
Claus Steinlein
Keyword(s):  
Chromosome Pair ◽  
Repetitive Sequences ◽  
5S Rdna ◽  
Mitotic Chromosomes ◽  
Banding Patterns ◽  
Single Chromosome ◽  
Replication Banding ◽  
Rdna Sequences ◽  
High Degree ◽  
Replication Bands

Mitotic chromosomes of 16 species of the frog genus Xenopus were prepared from kidney and lung cell cultures. In the chromosomes of 7 species, high-resolution replication banding patterns could be induced by treating the cultures with 5-bromodeoxyuridine (BrdU) and deoxythymidine (dT) in succession, and in 6 of these species the BrdU/dT-banded chromosomes could be arranged into karyotypes. In the 3 species of the clade with 2n = 20 and 4n = 40 chromosomes (X. tropicalis, X. epitropicalis, X. new tetraploid 1), as well as in the 3 species with 4n = 36 chromosomes (X. laevis, X. borealis, X. muelleri), the BrdU/dT-banded karyotypes show a high degree of homoeology, though differences were detected between these groups. Translocations, inversions, insertions or sex-specific replication bands were not observed. Minor replication asynchronies found between chromosomes probably involve heterochromatic regions. BrdU/dT replication banding of Xenopus chromosomes provides the landmarks necessary for the exact physical mapping of genes and repetitive sequences. FISH with an X. laevis 5S rDNA probe detected multiple hybridization sites at or near the long-arm telomeric regions in most chromosomes of X. laevis and X. borealis, whereas in X. muelleri, the 5S rDNA sequences are located exclusively at the long-arm telomeres of a single chromosome pair. Staining with the AT base pair-specific fluorochrome quinacrine mustard revealed brightly fluorescing heterochromatic regions in the majority of X. borealis chromosomes which are absent in other Xenopus species.

Cytogenetic studies on Sauria (Reptilia). I. Mitotic chromosomes of the Agamidae

1988 ◽  
Vol 9 (3) ◽  
pp. 301-310 ◽  
Author(s):  
E. Solleder ◽  
M. Schmid
Keyword(s):  
Chromosome Number ◽  
Chromosome Morphology ◽  
Mitotic Chromosomes ◽  
Metaphase Chromosomes ◽  
Banding Patterns ◽  
The Family ◽  
Telocentric Chromosomes ◽  
Cytogenetic Studies ◽  
Pericentric Inversions ◽  
Dna Organization

The karotypes of nine species of the family Agamidae were analyzed with various banding techniques and conventional cytogenetic stainings. Whereas the examined species of the genera Calotes and Leiolepis exhibit conservative karyotypes, the chromosome number and chromosome morphology varies considerably within the genus Agama. This is attributed to centric fusions between telocentric chromosomes and pericentric inversions within the chromosomes. None of the species demonstrated multiple quinacrine banding patterns in the euchromatic segments of the metaphase chromosomes. This is probably due to the special DNA organization in these organisms.

A new species of Trigonella sect. Ellipticae (Leguminosae-Papilionoideae) from Iran, including cytogenetic and anatomical notes

Phytotaxa ◽  
2015 ◽  
Vol 202 (1) ◽  
pp. 26 ◽  
Author(s):  
Massoud Ranjbar ◽  
ZAHRA HAJMORADI
Keyword(s):  
New Species ◽  
Chromosome Number ◽  
Related Species ◽  
Somatic Chromosome ◽  
Base Number ◽  
Somatic Chromosome Number ◽  
Chromosome Counts ◽  
Closely Related Species ◽  
A New Species

A new species, Trigonella bakhtiarica, from the Iranian province Chahar Mahal Va Bakhtiari is described, illustrated and compared to its most closely related species, T. aphanoneura. Trigonella bakhtiarica has a longer corolla and differs in the shape, surface and size of its pods, which are taxonomically informative characters in Trigonella sect. Ellipticae. Chromosome counts and meiosis assays show that both species are diploid, and that their euploid plants possess a somatic chromosome number of 2n = 2x = 16, which is consistent with the predicted base number of x = 8.

Sign in / Sign up

Export Citation Format

Share Document