Cytogenetical analysis of Clitoria ternatea

2021 ◽  
Vol 8 (1) ◽  
pp. 71-77
Author(s):  
P. Phukan ◽  
M. A. Laskar ◽  
B. J. Mylliemngap ◽  
J. M. Lamo
Keyword(s):  
Somatic Chromosome ◽  
Heterochromatic Region ◽  
Somatic Chromosome Number ◽  
Chromomycin A3 ◽  
Important Species ◽  
Clitoria Ternatea ◽  
Nucleolar Organizing Region ◽  
The Family ◽  
Cytogenetical Analysis

Clitoria ternatea L. of the family Fabaceae is an economical, ornamental as well as medicinal important species. Chromosome characterization of C. ternatea, encompassing karyomorphological as well as fluorochrome binding was carried out in the present investigation. Karyomorphological studies showed the presence of 2n = 16 somatic chromosome number with three pairs of metacentric chromosomes and five pairs of submedian chromosomes. The analysis also revealed the present of one pair of nucleolar organizing region or satellite. Fluorochrome binding using chromomycin A3 (CMA) and 4-6-diamidino-2-phenylindole (DAPI) showed the presence of GC- and AT- rich heterochromatic region. Further analysis revealed the percentage of GC-rich was comparatively higher than AT-rich heterochromatic region. This is the first report on heterochromatin characterization in C. ternatea.

scholarly journals FIRST KARYOTYPE REPORT ON Colocasia oresbia: A COMPARATIVE CYTOGENETIC STUDY BETWEEN TWO VARIETIES

2020 ◽  
Vol 31 (Issue 2) ◽  
pp. 7-11
Author(s):  
N. Begum Kazi ◽  
K. Dash Chandan ◽  
S. Sultana Syeda
Keyword(s):  
Cytogenetic Study ◽  
Chromosome Length ◽  
Future Research ◽  
Somatic Chromosome Number ◽  
Chromomycin A3 ◽  
Banding Patterns ◽  
Ecological Zones ◽  
Karyotypic Formula ◽  
Dapi Banding

Karyotypes of two Colocasia oresbia botanical varieties from Bangladesh were analyzed and compared with orcein, chromomycin A3 (CMA) and 4´-6 diamidino-2-phenylindole (DAPI). Both varieties had 2n=2x=26 chromosomes (karyotypic formula: 20m+6sm) and a pair of satellites each. Total chromosome length was 144.18±2.45 μm in C. oresbia var. oresbia and 133.02±2.75 μm in C. oresbia var. stolonifera. The karyotype of Colocasia oresbia var. oresbia is 2A whereas that of C. oresbia var. stolonifera is 1A. Six CMA and four DAPI bands were observed in C. oresbia var. oresbia and eight CMA and six DAPI bands in C. oresbia var. stolonifera. However, in these two morphologically distinct C. oresbia varieties of two different ecological zones, the same somatic chromosome number, diversification in various karyotypic parameters and CMA/DAPI-banding patterns were observed. In addition to taxonomic characters, the studied karyotype features will contribute to the characterization of these two C. oresbia varieties and to establish a base for future research. Key words: chromosome banding; CMA; DAPI; Karyotype.

A new diploid species of Lotus (Leguminosae) in Pakistan

1972 ◽  
Vol 50 (9) ◽  
pp. 1865-1870 ◽  
Author(s):  
Olga Sz.-Borsos ◽  
B. H. Somaroo ◽  
William F. Grant
Keyword(s):  
Somatic Chromosome ◽  
Diploid Species ◽  
Royal Botanic Garden ◽  
Tetraploid Species ◽  
Somatic Chromosome Number ◽  
Botanic Garden ◽  
Phenotypic Characters ◽  
Diploid Taxon ◽  
A New Species

Seed was received as Lotus corniculatus L. var. minor Bak. from Dr. B. L. Burtt of the Royal Botanic Garden, Edinburgh, who collected them from plants growing in Peshawar, Pakistan. Plants grown from this Peshawar seed all have a somatic chromosome number of 12. Measurement made on 15 phenotypic characters have been compared with six other diploid species of Lotus. From these analyses and a survey of the literature, it has been determined that these plants do not match any known diploid species, or variety, and are probably of the same taxon that Baker described as L. corniculatus L. var. minor. It is considered that this diploid taxon was incorrectly associated with the tetraploid species L. corniculatus L. s. str. (2n = 24). From a study of its nomenclature and the fact that the varietal description by Baker is too poor for the characterization of a species, the plants grown from the Peshawar seed have been described as a new species and named Lotus burttii Sz.-Borsos after Dr. B. L. Burtt, who collected this material. While its occurrence is so far not known outside of Pakistan, its complete area of distribution remains to be investigated.

scholarly journals Phenetic and genetic characterization of selected economically important species in the family Zingiberaceae

2018 ◽  
Vol 3 (1) ◽  
pp. 34
Author(s):  
A. B. W. R. Silva ◽  
H. Herath ◽  
S. P. Senanayake ◽  
D. B. R. Swarnathilaka
Keyword(s):  
Genetic Characterization ◽  
Important Species ◽  
The Family

scholarly journals Meloidogyne Virulence Locus Molecular Marker for Characterization of Selected Mi-Virulent Populations of Meloidogyne spp. Is Correlated with Several Genera of Betaproteobacteria

2011 ◽  
Vol 101 (4) ◽  
pp. 410-415 ◽  
Author(s):  
Laura Cortada ◽  
Hiromichi Sakai ◽  
Soledad Verdejo-Lucas ◽  
Takayuki Mizukubo
Keyword(s):  
Molecular Marker ◽  
Root Knot Nematodes ◽  
Important Species ◽  
Sequence Characterized Amplified Region ◽  
The Family ◽  
Eukaryotic Organism ◽  
Meloidogyne Spp ◽  
Mi Gene ◽  
In Silico Analyses

Resistance to root-knot nematodes in tomato is conferred by the Mi resistance gene to the three most important species of Meloidogyne: M. arenaria, M. incognita, and M. javanica. Nevertheless, the Mi gene is unable to inhibit the reproduction of selected and naturally Mi-virulent populations of root-knot nematodes. As pathogenicity assays are time consuming, molecular markers were developed for the easy identification of Mi-virulent populations of Meloidogyne. The sequence characterized amplified region-Meloidogyne virulence locus (MVC) molecular marker is reported to differentiate Mi-avirulent and naturally Mi-virulent from selected Mi-virulent populations. This marker was used to compare acquired virulence in populations of M. javanica from Spain. The original populations used to develop the MVC marker were included as control for reference. Results showed that this marker did not amplify genomic DNA extracted from single juveniles or females of any of the populations tested either from Spain or Japan. In silico analyses performed with the recently published complete genome of M. incognita, indicated that the MVC marker is not correlated to a MVC or to any eukaryotic organism but to several betaproteobacteria genus from the family Comamonadaceae.

scholarly journals Cytogenetic studies of three cultivated hill cotton (Gossypium arboreum L.) varieties from Bangladesh

2021 ◽  
Vol 81 (01) ◽  
pp. 135-138
Author(s):  
Afsana Hossain ◽  
Chandan Kumar Dash ◽  
Syeda Sharmeen Sultana
Keyword(s):  
Somatic Chromosome ◽  
Repetitive Sequences ◽  
Differential Staining ◽  
Gossypium Arboreum ◽  
Somatic Chromosome Number ◽  
Chromomycin A3 ◽  
Banding Patterns ◽  
Cytogenetic Studies ◽  
Karyotypic Diversity ◽  
Dapi Banding

Three hill cotton (Gossypium arboreum L.) varieties viz., HC-1, HC-2 and HC-3, released by Bangladesh Cotton Development Board were investigated through orcein, CMAand DAPI-banding for cytogenetical characterization and to elucidate the karyotypic diversity among these varieties. All these three varieties were found to possess 2n = 26 metacentric chromosomes with ‘1A’ karyotype. Based on TF%, AsK% and Syi index, HC-3 was little advanced over HC-1 and HC-2. These three varieties showed differential Chromomycin A3 (CMA)- and 4Ê-6 Diamidino-2-Phenyl Indole (DAPI)-banding patterns and a tendency of acumulation of repetitive sequences at the terminal regions was observed. Despite possessing same somatic chromosome number these three hill cotton varieties could be characterized by diversified karyotypic parameters through differential staining.

Prothrombin Metz : Purification and Characterization of a Variant of Human Prothrombin

1979 ◽  
Author(s):  
M Ribieto ◽  
J Elion ◽  
D Labie ◽  
F Josso
Keyword(s):  
Molecular Weight ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Factor Xa ◽  
Polyacrylamide Gel ◽  
Cleavage Pattern ◽  
Purification And Characterization ◽  
Double Immunodiffusion ◽  
The Family

For the purification of the abnormal prothrombin (Pt Metz), advantage has been taken of the existence in the family of three siblings who, being double heterozygotes for Pt Metz and a hypoprothrombinemia, have no normal Pt. Purification procedures included barium citrate adsorption and chromatography on DEAE Sephadex as for normal Pt. As opposed to some other variants (Pt Barcelona and Madrid), Pt Metz elutes as a single symetrical peak. By SDS polyacrylamide gel electrophoresis, this material is homogeneous and appears to have the same molecular weight as normal Pt. Comigration of normal and abnormal Pt in the absence of SDS, shows a double band suggesting an abnormal charge for the variant. Pt Metz exhibits an identity reaction with the control by double immunodiffusion. Upon activation by factor Xa, Pt Metz can generate amydolytic activity on Bz-Phe-Val-Arg-pNa (S2160), but only a very low clotting activity. Clear abnormalities are observed in the cleavage pattern of Pt Metz when monitored by SDS gel electrophoresis. The main feature are the accumulation of prethrombin l (Pl) and the appearance of abnormal intermediates migrating faster than Pl.

scholarly journals Plasma proteomics of green turtles (Chelonia mydas) reveals pathway shifts and potential biomarker candidates associated with health and disease

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
David P Marancik ◽  
Justin R Perrault ◽  
Lisa M Komoroske ◽  
Jamie A Stoll ◽  
Kristina N Kelley ◽  
...  
Keyword(s):  
Chelonia Mydas ◽  
Sea Turtle ◽  
Protein Abundance ◽  
Plasma Samples ◽  
Important Species ◽  
Green Turtles ◽  
Rehabilitation Programs ◽  
Potential Biomarker ◽  
Health And Disease

Abstract Evaluating sea turtle health can be challenging due to an incomplete understanding of pathophysiologic responses in these species. Proteome characterization of clinical plasma samples can provide insights into disease progression and prospective biomarker targets. A TMT-10-plex-LC–MS/MS platform was used to characterize the plasma proteome of five, juvenile, green turtles (Chelonia mydas) and compare qualitative and quantitative protein changes during moribund and recovered states. The 10 plasma samples yielded a total of 670 unique proteins. Using ≥1.2-fold change in protein abundance as a benchmark for physiologic upregulation or downregulation, 233 (34.8%) were differentially regulated in at least one turtle between moribund and recovered states. Forty-six proteins (6.9%) were differentially regulated in all five turtles with two proteins (0.3%) demonstrating a statistically significant change. A principle component analysis showed protein abundance loosely clustered between moribund samples or recovered samples and for turtles that presented with trauma (n = 3) or as intestinal floaters (n = 2). Gene Ontology terms demonstrated that moribund samples were represented by a higher number of proteins associated with blood coagulation, adaptive immune responses and acute phase response, while recovered turtle samples included a relatively higher number of proteins associated with metabolic processes and response to nutrients. Abundance levels of 48 proteins (7.2%) in moribund samples significantly correlated with total protein, albumin and/or globulin levels quantified by biochemical analysis. Differentially regulated proteins identified with immunologic and physiologic functions are discussed for their possible role in the green turtle pathophysiologic response and for their potential use as diagnostic biomarkers. These findings enhance our ability to interpret sea turtle health and further progress conservation, research and rehabilitation programs for these ecologically important species.

Sign in / Sign up

Export Citation Format

Share Document