Molecular and Ultrastructural Analysis of a Porocephalus sp. (Arthropoda: Pentastomida) Removed from a Rattlesnake

2021 ◽  
Vol Vol 66 (1) (January (1)) ◽  
pp. 1-5
Author(s):  
Jerome Goddard ◽  
Gerald Baker ◽  
Petra Jericke ◽  
Lawrence Birchman ◽  
Ethan Woodward ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Adult Female ◽  
Sequence Data ◽  
Molecular Data ◽  
Ultrastructural Analysis ◽  
Oxidase Subunit ◽  
Data Support ◽  
Partial 18S

Ultrastructural and molecular data are provided from a single adult female pentastomid opportunistically collected from a road-killed rattlesnake in Russell, KS. Ultrastructural data consisted of light and SEM microscopy of the pentastomid and its eggs, while molecular data consisted of partial 18S and 28S ribosomal sequences and a partial cytochrome c oxidase subunit 1 sequence from the same specimen used for SEM. Ultrastructural and molecular data support generic identification of the pentastomid as Porocephalus sp. These molecular data were also used with previously published pentastomid sequence data for a concatenated phylogenetic analysis, which support the current, morphology-based taxonomic placement of the genus.

scholarly journals Molecular analysis of populations of the invasive species Rhynchophorus ferrugineus Olivier, 1790 (Coleoptera: Curculionidae) in the Region of Murcia (Spain)

2017 ◽  
pp. 155-176
Author(s):  
Miguel Lozano-Terol ◽  
María Juliana Rodríguez-García ◽  
José Galián
Keyword(s):  
Invasive Species ◽  
Phylogenetic Analysis ◽  
Mitochondrial Dna ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Molecular Analysis ◽  
Cox1 Gene ◽  
Rhynchophorus Ferrugineus ◽  
Phylogeographic Analysis ◽  
Oxidase Subunit

En este estudio se analizan dos fragmentos del gen de la citocromo c oxidasa subunidad I (COX1) del ADN mitocondrial de 61 individuos del género Rhynchophorus colectados en la Región de Murcia a fin de determinar su procedencia. El análisis filogenético del fragmento 1 de las muestras de la Región de Murcia conjuntamente con las secuencias disponibles en GenBank indica que los individuos corresponden a la especie Rhynchophorus ferrugineus.Las secuencias de Murcia se colapsan en un único haplotipo (H8 mediterráneo) que aparece dentro del clado de R. ferrugineus. De los análisis filogeográficos se infiere que el origen de los individuos de Murcia es Egipto. Adicionalmente, se examinó una región contigua del COX1 (fragmento 2) en la que las secuencias se colapsaron en dos haplotipos. In this research two fragments of the cytochrome c oxidase subunit I (COX1) gene of the mitochondrial DNA were analyzed in 61 individuals of the genus Rhynchophorus collected in the Region of Murcia with the aim of determining their origin. Phylogenetic analysis of fragment 1 of the samples collected in the Region of Murcia together with the available sequences in GenBank, indicated that these individuals correspond to the species R. ferrugineus. Sequences from Murcia collapsed into the H8 Mediterranean haplotype, which cluster into the R. ferrugineus clade. Phylogeographic analysis shows that the origin of the individuals collected in the Region of Murcia is Egypt. Additionally, a contiguous fragment of COX1 (fragment 2) was analyzed and the sequences collapsed into two haplotypes.

scholarly journals Three new species of the primitively segmented spider genus Songthela (Araneae, Mesothelae) from Guizhou Province, China

ZooKeys ◽  
2021 ◽  
Vol 1037 ◽  
pp. 57-71
Author(s):  
Zhaoyang Chen ◽  
Dengqing Li ◽  
Daiqin Li ◽  
Xin Xu
Keyword(s):  
New Species ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Dna Barcode ◽  
Morphological Characters ◽  
Molecular Data ◽  
Genetic Distances ◽  
Guizhou Province ◽  
Oxidase Subunit ◽  
Three New Species

We diagnose and describe three new species of the primitively segmented spider genus Songthela from Guizhou Province, China, based on morphological characters and molecular data: S. liuisp. nov. (♂♀), S. tianzhusp. nov. (♂♀), and S. yupingsp. nov. (♂♀). We provide the genetic distances within and among the three new species based on the DNA barcode gene, cytochrome c oxidase subunit I (COI) to support our descriptions. We also provide the COI GenBank accession codes for the three new species for future identification.

A new species of planthopper in the genus Agoo (Hemiptera: Fulgoroidea: Derbidae) from coquito palms (Astrocaryum alatum) in Costa Rica

Zootaxa ◽  
2020 ◽  
Vol 4779 (3) ◽  
pp. 409-418 ◽  
Author(s):  
BRIAN W. BAHDER ◽  
MARCO A. ZUMBADO ECHAVARRIA ◽  
EDWIN A. BARRANTES BARRANTES ◽  
GERNOT KUNZ ◽  
ERICKA E. HELMICK ◽  
...  
Keyword(s):  
New Species ◽  
Costa Rica ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Sequence Data ◽  
The Novel ◽  
La Selva ◽  
Biological Station ◽  
Oxidase Subunit ◽  
A New Species

An ongoing survey to document planthopper diversity on palms (Arecaceae) is being conducted in Costa Rica. During these efforts a new species of derbid planthopper belonging to the genus Agoo was found on Astrocaryum alatum Loomis in the Heredia province at La Selva Biological Station and is described here as Agoo luzdenia Bahder & Bartlett sp. n., bringing the genus to four described taxa—A. dahliana, A. luzdenia Bahder & Bartlett sp. n., A rubrimarginata, and A. xavieri. Sequence data for the cytochrome c oxidase subunit I (COI) and 18S genes was generated for the novel taxon and strongly supports its placement in the genus Agoo. 

A new species of Achalinus (Squamata: Xenodermidae) from Trang An Landscape Complex, Ninh Binh Province, Vietnam

Zootaxa ◽  
2020 ◽  
Vol 4877 (1) ◽  
pp. 174-184
Author(s):  
VINH QUANG LUU ◽  
THOMAS ZIEGLER ◽  
NGHIA VAN HA ◽  
OANH VAN LO ◽  
TUOI THI HOANG ◽  
...  
Keyword(s):  
New Species ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Adult Female ◽  
Anterior Region ◽  
Mitochondrial Cytochrome ◽  
Female Specimen ◽  
Unique Combination ◽  
Oxidase Subunit ◽  
A New Species

We describe a new snake of the genus Achalinus based on an adult female specimen from Ninh Binh Province, Vietnam. Achalinus tranganensis sp. nov. is differentiated from its congeners genetically and by a unique combination of the following characters: 1) maxillary teeth 29; 2) suture between the internasals distinctly longer than that between the prefrontals; 3) loreal distinctly wider than high, extending from the nasal to the eye; 4) dorsal scales in 25–23–23 rows, keeled; 5) two pairs of prefrontals; 6) supralabials six; 7) infralabials six; 8) temporals 2+3, the two anterior temporals in contact with eye; 9) ventrals 171; 10) subcaudals 73+, unpaired; 11) cloacal plate entire; 12) dorsum in preservative reddish to greyish brown above; 13) the lower part of head side and chin region somewhat paler than the dorsum; 14) venter greyish cream, with the anterior region of each ventral and subcaudal somewhat darker. Based on molecular comparisons using a fragment of the mitochondrial cytochrome c oxidase subunit I (COI), the new species differs from other members of the genus by at least 11.8%. This discovery increases the number of Achalinus species known from Vietnam to seven. 

Adult and larval associations of the alpine stonefly genus Riekoperla McLellan (Plecoptera:Gripopterygidae) using mitochondrial DNA

2011 ◽  
Vol 25 (1) ◽  
pp. 11 ◽  
Author(s):  
J. H. Mynott ◽  
J. M. Webb ◽  
P. J. Suter
Keyword(s):  
Mitochondrial Dna ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Aquatic Insects ◽  
Sequence Data ◽  
New South ◽  
Mitochondrial Gene ◽  
Life Stage ◽  
Molecular Data ◽  
South Wales

The current taxonomic understanding of the genus Riekoperla McLellan, 1971 (Gripopterygidae) is poor, with 15 of the 28 species and subspecies having unknown or uncertain larval associations. Sequences of a 657 bp fragment from the mitochondrial gene cytochrome c oxidase subunit 1 (CO1) were obtained from 122 specimens of 13 species collected throughout the alpine areas of New South Wales and Victoria, Australia. Of these, sequence data associated adults and larvae for the following 10 species: R. alpina McLellan, 1971, R. cf. intermedia, R. compressa Theischinger, 1985, R. hynesorum Theischinger, 1985, R. karki McLellan, 1971, R. montana Theischinger, 1985, R. reticulata (Kimmins, 1951), R. rugosa (Kimmins, 1951), R. trapeza Theischinger, 1985, and R. tuberculata McLellan, 1971. Adults of R. intermedia Theischinger, 1985, R. triloba triloba McLellan, 1971 and R. williamsi McLellan, 1971 were sequenced but no larvae were associated with them. The 13 species were reciprocally monophyletic and had minimum interspecific sequence divergences ranging from 7.2–19.5%, higher than the maximum intraspecific sequence divergences (0.6–5.8%). The combination of morphology and molecular data enabled rapid life stage association for alpine Riekoperla species and this method should be used more frequently for other environmentally significant aquatic insects.

Addendum to “Leaf insects from Luzon, Philippines, with descriptions of four new species, the new genus Pseudomicrophyllium, and redescription of Phyllium (Phyllium) geryon Gray, 1843, (Phasmida: Phylliidae)”

Zootaxa ◽  
2018 ◽  
Vol 4433 (2) ◽  
pp. 389
Author(s):  
ROYCE T. CUMMING ◽  
JING V. LEONG ◽  
DAVID J. LOHMAN
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
New Genus ◽  
Dna Barcode ◽  
Oxidase Subunit

At the time Cumming et al. (2017) was published, the GenBank accession numbers for partial cytochrome c oxidase subunit I (COI) DNA barcode sequences from Microphyllium specimens sampled in the phylogenetic analysis were not yet available. This information is provided in Table 1. 

Sign in / Sign up

Export Citation Format

Share Document