Comparison of Discolaimium (Nematoda: Dorylaimida) from South Africa using 18S rDNA

Summary A nematode survey conducted in the Telperion Nature Reserve (Mpumalanga, South Africa) resulted in a new Ironus species being described as well as two known species reported for the first time from South Africa, viz., Chronogaster aspinata and Paraphanolaimus behningi. Ironus telperionensis n. sp. is characterised by a long (1724 (1456-1864) μm) and slender body (50 times longer than mid-body diam.), long stoma (116 (87-129) μm), and a 235 (212-257) μm long tail with a ventral curl at the tip. The new species is morphologically most similar to I. ignavus and I. paludicola. However, the new species can be separated from I. ignavus based on the lower ratio c (7.3 (6.5-8.0) vs 8.0-11.0) and longer stoma (116 (87-129) vs 76-90 μm). Ironus telperionensis n. sp. can be distinguished from I. paludicola by the lower ratio a (49.2 (37.4-53.0) vs 60.0-80.0) and lower ratio c (7.3 (6.5-8.0) vs 8.0-13.0). The 18S rDNA phylogenetic analysis of I. telperionensis n. sp. placed it close to I. elegans (KC133064), whereas the 28S rDNA phylogenetic analysis placed it close to I. tenuicaudatus. The phylogenetic analysis revealed that Ironus is a monophyletic group. Paraphanolaimus behningi was identified based on anteriorly bent vagina in the female, spicule length (106 or 110 μm) and number of supplements (13 or 14) in the male. Chronogaster aspinata was identified based on body length (1127 or 1203 μm), cephalic setae length (7 μm), finely rounded tail terminus without a mucron or additional spines, and no longitudinal ridges. It fits the known description of the species. Although various differences were observed between previously reported populations of these species, the morphometrics of the specimens found in Telperion widen the existing variation in these two species.

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Redescription of a predatory and cannibalistic nematode, Butlerius butleri Goodey, 1929 (Rhabditida: Diplogastridae), from South Africa, including its first SEM study

Nematology ◽

10.1163/15685411-bja10089 ◽

2021 ◽

pp. 1-18

Author(s):

Ebrahim Shokoohi ◽

Joaquín Abolafia

Keyword(s):

South Africa ◽

South African ◽

Molecular Analysis ◽

18S Rdna ◽

African Population ◽

Soil Nematodes ◽

South African Population ◽

Free Living ◽

Sem Study ◽

First Time

Summary Butlerius butleri was isolated during a survey on free-living soil nematodes from South Africa. The South African population of B. butleri is characterised by having a large stoma divided into two chambers and bearing a thorn-like dorsal tooth, spicules 44 (41-49) μm long, gubernaculum 29 (28-30) μm long, three pairs of precloacal papillae and five pairs of postcloacal papillae, and a filiform tail 280 (204-332) μm long in females, 310 (256-356) μm long in males. The SEM observation showed the cheilostom is divided into six conical plates 2.1-2.3 μm long. Nblast of 18S rDNA revealed 99% similarity and 6-7 bp differences with the B. butleri (KP453998) from Iran. Molecular analysis of B. butleri based on the sequence of the 18S and 28S rDNA placed it together with Butlerius and close to Pseudodiplogasteroides and Diplogasteroides. This species showed predatory and cannibalistic behaviours, feeding on other B. butleri in the substrate. SEM photographs of the species are presented for the first time.

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Description of Calcaridorylaimus heynsi n. sp. (Nematoda: Dorylaimidae) from Potchefstroom, South Africa

Nematology ◽

10.1163/15685411-00003346 ◽

2020 ◽

Vol 22 (8) ◽

pp. 855-865

Author(s):

Antoinette Swart ◽

Hendrika Fourie ◽

Louwrens R. Tiedt ◽

Milad Rashidifard

Keyword(s):

South Africa ◽

Phylogenetic Analysis ◽

New Species ◽

18S Rdna ◽

Molecular Techniques ◽

28S Rdna ◽

Median Line ◽

Rdna Sequences ◽

28S Rdna Sequences ◽

Short Body

Summary Calcaridorylaimus heynsi n. sp. is the second species of the genus to be described by both morphological and molecular techniques. Morphologically, it can be distinguished from all known species of Calcaridorylaimus by a combination of the following characters: presence of advulval ornamentations, short body (0.90-1.33 mm), slightly anteriorly positioned vulva (V = 47.6 (45.8-49.8)), short odontostyle in females and males (13.1 (11.5-14.5) μm and 13.5 (12.0-18.0) μm, respectively), number of supplements (2 + 9-11), short spicules when measured along the median line (40.4 (38-42) μm) and pore-like vulval opening. It is closest to C. sirgeli, especially in the presence of advulval ornamentations and the pore-like vulva. Phylogenetic analysis based on partial D2-D3 segment of 28S rDNA sequences showed that C. heynsi n. sp. is in a well-supported sister relation with Mesodorylaimus sp. in a clade with C. cignatus and Mesodorylaimus spp. In the Bayesian tree, using partial sequences 18S rDNA, M. japonicus was the closest taxon to the new species.

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Molecular technique reveals high variability of 18S rDNA distribution in harvestmen (Opiliones, Phalangiidae) from South Africa

Comparative Cytogenetics ◽

10.3897/compcytogen.v12i1.21744 ◽

2018 ◽

Vol 12 (1) ◽

pp. 41-59 ◽

Cited By ~ 6

Author(s):

František Šťáhlavský ◽

Vera Opatova ◽

Pavel Just ◽

Leon N. Lotz ◽

Charles R. Haddad

Keyword(s):

South Africa ◽

18S Rdna ◽

High Variability ◽

Molecular Technique

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Time and Latitude in South Africa

International Astronomical Union Colloquium ◽

10.1017/s0252921100026816 ◽

1972 ◽

Vol 1 ◽

pp. 27-38

Author(s):

J. Hers

Keyword(s):

South Africa ◽

Cape Town ◽

Astronomical Observations ◽

Royal Observatory ◽

The Republic ◽

Astronomical Determination ◽

Limited Accuracy ◽

Better Than

In South Africa the modern outlook towards time may be said to have started in 1948. Both the two major observatories, The Royal Observatory in Cape Town and the Union Observatory (now known as the Republic Observatory) in Johannesburg had, of course, been involved in the astronomical determination of time almost from their inception, and the Johannesburg Observatory has been responsible for the official time of South Africa since 1908. However the pendulum clocks then in use could not be relied on to provide an accuracy better than about 1/10 second, which was of the same order as that of the astronomical observations. It is doubtful if much use was made of even this limited accuracy outside the two observatories, and although there may – occasionally have been a demand for more accurate time, it was certainly not voiced.

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Indirect Rule in South Africa

10.1017/upo9781580467421 ◽

2009 ◽

Author(s):

J. C. Myers

Keyword(s):

South Africa ◽

Indirect Rule

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Combining Professional Interests and Travel: People to People Citizen Ambassador Program

Perspectives on Global Issues in Communication Sciences and Related Disorders ◽

10.1044/gics1.2.47 ◽

2011 ◽

Vol 1 (2) ◽

pp. 47-54

Author(s):

Alex Johnson ◽

Amanda Hitchins

Keyword(s):

South Africa ◽

Health Education ◽

Communication Disorders ◽

Social Conditions ◽

Speech Language Pathologists ◽

Exchange Program

Abstract This article summarizes a series of trips sponsored by People to People, a professional exchange program. The trips described in this report were led by the first author of this article and include trips to South Africa, Russia, Vietnam and Cambodia, and Israel. Each of these trips included delegations of 25 to 50 speech-language pathologists and audiologists who participated in professional visits to learn of the health, education, and social conditions in each country. Additionally, opportunities to meet with communication disorders professionals, students, and persons with speech, language, or hearing disabilities were included. People to People, partnered with the American Speech-Language-Hearing Association (ASHA), provides a meaningful and interesting way to learn and travel with colleagues.

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