Nucleic Acid and Phosphoester Hydrolytic Cleavage Catalysed by Aza-Crown Ether Metal Complexes as Synthetic Nucleases

2014 ◽  
Vol 39 (3) ◽  
pp. 209-232 ◽  
Author(s):  
Fang-zhen Li ◽  
Fa-mei Feng ◽  
Lan Yu ◽  
Jia-qing Xie
Keyword(s):  
Nucleic Acid ◽  
Crown Ether ◽  
Metal Complexes ◽  
Hydrolytic Cleavage ◽  
Synthetic Nucleases ◽  
Aza Crown Ether

Development of the aza-crown ether metal complexes as artificial hydrolase

2016 ◽  
Vol 154 ◽  
pp. 89-102 ◽  
Author(s):  
Lan Yu ◽  
Fang-zhen Li ◽  
Jiao-yi Wu ◽  
Jia-qing Xie ◽  
Shuo Li
Keyword(s):  
Crown Ether ◽  
Metal Complexes ◽  
Aza Crown Ether

Transition Metal Complexes of a Diaza-Crown Ether with two Carbamoylmethyl Substituents: Synthesis and Assessment as a Functional Nuclease

2017 ◽  
Vol 42 (2) ◽  
pp. 136-144
Author(s):  
Jia-qing Xie ◽  
Shu-lan Cai ◽  
Fa-mei Feng
Keyword(s):  
Transition Metal ◽  
Crown Ether ◽  
Metal Complexes ◽  
Transition Metal Complexes ◽  
Dna Cleavage ◽  
Catalytic Mechanism ◽  
Active Species ◽  
Lewis Acidity ◽  
Hydrolytic Cleavage ◽  
Central Metal

We report the synthesis, catalytic function and catalytic mechanism of two transition metal complexes (CuL, ZnL) of a diaza-crown ether with two acetylamino side arms [L = 2,2′-(1,4,10,13-tetraoxa-7,16-diazacyclooctadecane-7,16-diyl)diacetamide] in the hydrolysis of DNA. Their nuclease functions on pUC19 DNA cleavage were investigated. The results indicated that the active species might be formed by the deprotonation of the water-coordinated molecules in the complex and the optimum pH is 8.0 for both CuL and ZnL. The catalytic activity of CuL is higher than that of ZnL in DNA hydrolytic cleavage due to the difference in the Lewis acidity of the central metal ions, which is contrary to the result with the Cu and Zn complexes of the parent ligand L0 (1,4,10,13-tetraoxa-7,16-diazacyclooctadecane) as artificial nuclease. Comparison studies of DNA cleavage in the presence and absence of several oxygen scavengers showed that these complexes can promote DNA cleavage by a hydrolytic pathway. Our proposed mechanism suggests that the negative charge on the phosphorus oxygen atom of the substrate molecule is dispersed and the intermediate is formed and stabilised by hydrogen bonding between the DNA molecule and the acetylamino group of the complex.

Complexation and transport of amino acid esters and their salts with synthesised chiral novel aza crown ether derivatives

2013 ◽  
Vol 26 (5-6) ◽  
pp. 363-372 ◽  
Author(s):  
Mehmet Kazaylek ◽  
Şafak Ozhan Kocakaya ◽  
Elif Varhan Oral ◽  
Mehmet Çolak ◽  
Mehmet Karakaplan
Keyword(s):  
Amino Acid ◽  
Crown Ether ◽  
Amino Acid Esters ◽  
Acid Esters ◽  
Aza Crown Ether

Stabilization of nucleic acid secondary structure by cationic metal complexes

Biochemistry ◽  
1980 ◽  
Vol 19 (3) ◽  
pp. 504-512 ◽  
Author(s):  
Richard L. Karpel ◽  
Arthur H. Bertelsen ◽  
Jacques R. Fresco
Keyword(s):  
Nucleic Acid ◽  
Secondary Structure ◽  
Metal Complexes
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