TCR-mutations in peripheral blood lymphocytes and immune status in individuals exposed to chronic radiation exposure

2019 ◽  
Keyword(s):  
Radiation Exposure ◽  
Peripheral Blood ◽  
Immune Status ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Chronic Radiation ◽  
Chronic Radiation Exposure

Apoptosis of Lymphocytes and Polymorphisms of Apoptosis Regulation Genes in Individuals Exposed to Chronic Radiation Exposure

2020 ◽  
Vol 65 (4) ◽  
pp. 36-42
Author(s):  
E. Blinova ◽  
A. Kotikova ◽  
M. Yanishevskaya ◽  
A. Akleev
Keyword(s):  
Radiation Exposure ◽  
Allelic Variation ◽  
Early Stage ◽  
Peripheral Blood Lymphocytes ◽  
Postnatal Period ◽  
Blood Lymphocytes ◽  
Chronic Radiation ◽  
Techa River ◽  
Chronic Radiation Exposure ◽  
Number Of Cells

Purpose: Study the apoptotic death of peripheral blood lymphocytes in long-term period in persons exposed to chronic radiation exposure, and analysis of association of the polymorphic regions rs4645878, rs2279115, rs28362491, rs664677, rs1042522, rs1801270, rs2279744 of the BAX, BCL2, NFkB, ATM, TP53, CDKN1A, MDM2 genes with apoptotic lymphocytes frequency in residents of the coastal villages of the Techa River. Material and methods: The study of apoptosis and genotyping was conducted in 390 persons exposed to chronic radiation exposure as a result of Mayak PA radioactive waste releases into the Techa–Iset–Tobol river system. The early stage of apoptosis was assessed on a flow cytometer by the presence of phosphatidylserine on the surface of the cell membranes using the Annexin V Apoptosis Detection Kit I and the late stage of apoptosis using the TUNEL method. Real-time PCR genotyping was performed of allelic variations of rs4645878, rs2279115, rs28362491, rs664677, rs1042522, rs1801270, rs2279744 of BAX, BCL2, NFkB, ATM, TP53, CDKN1A, MDM2 genes in a group of irradiated individuals. Results: The number of cells at the early stage of apoptosis is statistically significantly increased in individuals whose irradiation began during the period of intrauterine development and continued in the postnatal period compared to individuals exposed only in the postnatal period. At the same time, the number of lymphocytes at the stage of DNA fragmentation in the group irradiated in utero is lower than in the group irradiated postnatally and non-irradiated individuals. Also, a weak negative correlation between intrauterine doses of RBM irradiation and the doses of thymus and peripheral lymphoid organs with the number of cells in the late stage of apoptosis in individuals irradiated in utero. The influence of allelic variation rs4645878 of the BAX gene was established on the number of lymphocytes at the early stage of apoptosis in residents of coastal villages of the Techa River. A statistically significant decrease in the number of cells at an early stage of apoptosis is observed in C/C genotype carriers according to the allelic variation rs4645878 of the BAX gene compared with carriers of the T/T and T/C genotypes. Conclusion: Residents of coastal villages of the Techa River exposed to radiation during the period of prenatal development, there are differences in the frequency of apoptotic death of peripheral blood lymphocytes compared with non-irradiated persons and persons who were irradiated in the postnatal period. SNPs of apoptosis-regulating genes can modify the response of blood lymphocytes to radiation in a wide range of doses RBM.

scholarly journals CSF-1-Induced DC-SIGN+ Macrophages Work In The Ovarian Endometriosis

2021 ◽  
Author(s):  
Xiaocui Li ◽  
Wei Hong ◽  
Yunlang Cai ◽  
Zhenzhen Zheng ◽  
Min An
Keyword(s):  
Peripheral Blood ◽  
Peritoneal Fluid ◽  
Immune Status ◽  
Biological Activities ◽  
Peripheral Blood Lymphocytes ◽  
Enzyme Linked Immunosorbent Assay ◽  
Predominant Role ◽  
Blood Lymphocytes ◽  
Suppressive Phenotype

Abstract BackgroundCSF-1 was found to be accumulated in the lesions and peritoneal fluid of endometriosis patients, and CSF-1 induced THP-1-derived macrophages to polarize toward a suppressive phenotype. Researchers found that macrophages were the predominant cells in the peritoneal fluid (PF) of endometriosis patients, and the primary consensus is that the immune status in the PF of endometriosis patients exhibits a depressed state. Does the cytokine CSF-1 induce monocytes to differentiate into macrophages with a DC-SIGN+ suppressive phenotype in endometriosis?MethodsThe level of CSF-1 in control endometrium (N=11), eutopic endometrium (N=17), and ectopic (N=39) endometrium of endometriosis patients was evaluated by real-time polymerase chain reaction and in the PF of control (N=25) and endometriosis (N=35) patients by enzyme-linked immunosorbent assay. CSF-1 was examined by a MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel in an in vivo study. DC-SIGN+ suppressive macrophages were detected by immunohistochemical staining of tissues and flow cytometric analysis of the PF of control (N=25) and endometriosis (N=35) patients. The phenotypes and biological activities of the resulting macrophages derived from THP-1 cells induced by CSF-1 were compared by an in vitro coculture system with peripheral blood lymphocytes from normal subjects.Results In this study, we found the proportion of DC-SIGN+ suppressive macrophages was larger in the abdominal immune microenvironment of endometriosis patients. CSF-1 was primarily secreted from the ectopic lesions and peritoneum of mice with endometriosis. And, CSF-1 induced the polarization of macrophages toward a DC-SIGN+ suppressive phenotype; this effect was abolished by the addition of anti-CSF-1R. CSF-1 induced DC-SIGN+ macrophages, leading to a depressed status of peripheral blood lymphocytes, including a high percentage of Treg cells and a low percentage of CD8+ T cells. Similarly, blockade with anti-CSF-1R abrogated this biological effect. This is the first study on the predominant role of DC-SIGN+ suppressive macrophages in the depressed immune status of endometriosis patients.Conclusions This is the first study on the predominant role of DC-SIGN+ suppressive macrophages in the depressed immune status of endometriosis patients. Further study of the mechanism and biological activities of CSF-1-induced DC-SIGN+ suppressive macrophages will enhance our understanding of the physiology of endometriosis and indicate new directions for further study.

scholarly journals Evaluation of Peripheral Blood Lymphocytes Proliferation in Botteng Village, Mamuju Inhabitants Using Binucleate Index

2017 ◽  
Vol 12 (2) ◽  
pp. 107
Author(s):  
Dwi Ramadhani ◽  
Sri Sardini ◽  
Masnelli Lubis ◽  
Mukh Syaifudin
Keyword(s):  
Radiation Exposure ◽  
Peripheral Blood ◽  
Healthy Adult ◽  
Background Radiation ◽  
Peripheral Blood Lymphocytes ◽  
Small Sample ◽  
Control Group ◽  
Sample Number ◽  
Blood Lymphocytes ◽  
Study Results

Botteng Village in Mamuju, West Sulawesi was known for the high natural background radiation exposure. Botteng Village inhabitants exposed to high natural radiation in their daily life. Radiation exposure can inhibit the mitosis mechanism at various phases. Our previous study revealed that mitotic and nuclear division indexes in Botteng Village inhabitants were lower compared to control samples. To validate our previous study results here we evaluate the binucleate index in peripheral blood lymphocytes of Botteng Village inhabitants. Blood samples were collected from thirteen healthy adult subjects in Botteng Village and thirteen healthy adult subjects in normal background radiation area. Binucleate index was calculated as the proportion of binucleated cell (BNC) in 500 cells for each sample. Our study showed that the BI in Botteng Village was higher compared to control group (23.58 ± 9.60 vs 23.47 ± 6.24). Statistical analysis revealed that the different was not significant (p=0.973). It is possible that the small sample numbers used in this study were not adequate to represent the BI value in Botteng Village inhabitants. This study also showed that there was insignificant difference of BI in respect to gender and age for all samples. Further study using larger sample number should be conducted to ensure the possibility of BI to evaluate the effect of chronic low radiation dose exposure on lymphocytes proliferation.

scholarly journals CHANGES OF CYCLIN D1-DEPENDENT REGULATION OF CELL CYCLE IN PERIPHERAL BLOOD LYMPHOCYTES OF CHORNOBYL CLEAN-UP WORKERS AT A REMOTE PERIOD AFTER RADIATION EXPOSURE

2020 ◽  
Vol 25 ◽  
pp. 430-442
Author(s):  
N. Golyarnik ◽  
◽  
I. Ilyenko ◽  
L. Zvarych ◽  
D. Bazyka ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Radiation Exposure ◽  
Cyclin D1 ◽  
Peripheral Blood ◽  
Genome Instability ◽  
Peripheral Blood Lymphocytes ◽  
Control Group ◽  
Proliferative Potential ◽  
Blood Lymphocytes ◽  
Regulation Of Cell Cycle

Objective. To study proliferative potential of peripheral blood lymphocytes of Chornobyl clean-up workers by level of expression of cyclin D1 and quantitative parameters of cell cycle at a remote period after radiation exposure. Materials and methods. The research subject was the peripheral blood lymphocytes (PB) of Chornobyl clean-up workers 30–33 years after radiation exposure. A total of 207 men were surveyed, 164 of them were clean-up workers exposed in the dose range 10.43–3623.31 mSv and 43 persons of the control group. Analysis of proliferation potential (cell cycle initiation) and cyclin D1 expression in PB lymphocytes were performed in vitro by a micro method of whole blood leukocytes culture with phytohemagglutinine-P (PHA). Sample preparation was performed by a standard immunofluorescent assay for intracellular proteins using the FITC labelled Mouse Anti-Human Cyclin D1 Antibody Set. Cell distribution by cell cycle phases studied by propidium iodide DNA staining and analysis on FACSCalibur laser flow cytometer in histogram mode with separation of G0/G1-, S- and G2/M-regions and Sub-G0/G1- region (apoptotic cells). Results and conclusions. An increase in the level of spontaneous сyclin D1 expression and disturbance of сyclin D1-dependent regulation of cell cycle of PB lymphocytes after mitogen activation were determined in a remote period after radiation exposure. An increase in the level of cyclin D1 expression was accompanied by increase in pool of cells in the S- and G2/M-phases of cell cycle which characterizes the high proliferative potential of PB lymphocytes. Mitogen-induced delay of cell cycle of lymphocytes in G1/S check point and reduction of S-phase was revealed. These changes are a manifestation of genomic instability caused by the effect of radiation and depend on the radiation dose. The results confirm the hypothesis about the significance of levels of cyclin D1 expression, as a criterion for manifestations of genome instability and risks of oncogenesis in a remote period after irradiation. Key words: cell cycle, cell proliferation, cyclin D1, genome instability, radiation exposure, Chornobyl clean-up workers.

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