scholarly journals Yellow streak of rakkyo(Allium chinense G.Don), a newly recognized disease caused by garlic latent virus and onion yellow dwarf virus.

1991 ◽  
Vol 57 (1) ◽  
pp. 65-69 ◽  
Author(s):  
Isamu SAKO ◽  
Wataru NAKASONE ◽  
Kiyotugu OKADA ◽  
Satoshi T. OHKI ◽  
Takeshi OSAKI ◽  
...  
Keyword(s):  
Latent Virus ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Onion Yellow Dwarf Virus ◽  
Garlic Latent Virus ◽  
Yellow Dwarf Virus ◽  
Allium Chinense

scholarly journals Double Infection of Onion yellow dwarf virus and Shallot latent virus in Garlic from Several Regions in Indonesia

2021 ◽  
Vol 25 (1) ◽  
pp. 40
Author(s):  
Nurenik Nurenik ◽  
Sedyo Hartono ◽  
Sri Sulandari ◽  
Susamto Somowiyarjo ◽  
Argawi Kandito
Keyword(s):  
Early Detection ◽  
Latent Virus ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Onion Yellow Dwarf Virus ◽  
Rt Pcr ◽  
Double Infection ◽  
Specific Primers ◽  
Shallot Latent Virus ◽  
Yellow Dwarf Virus

Viruses have been a problem on garlic cultivations in various countries. There are several viruses reported infecting garlic. Genera Potyvirus and Carlavirus are the most common viruses found infecting garlic. Mixed infection on garlic is often designated as a “garlic viral complex”. These viruses can be transmitted through imported garlic seeds. Therefore, it is necessary to conduct early detection of garlic seeds to prevent the epidemic of these viruses. This study aimed to detect Onion yellow dwarf virus (OYDV) and Shallot latent virus (SLV) on garlic. Garlic samples were obtained from Enrekang, Magelang, Temanggung, Tawangmangu, and Yogyakarta. Total RNA was extracted from the samples and subsequently used for RT-PCR using two pairs of specific primers SLV-F/SLV-R and OYDV-F/OYDV-R. Primary pair SLV-F/SLV-R in amplicons sized 276 bp, while OYDV-F/OYDV-R in amplicons sized 112 bp. RT-PCR results showed that OYDV was found in all samples tested in this study. Meanwhile, double infections (OYDV and SLV) were found in eight out of ten samples tested. These results indicated that double infections on garlic were common in Indonesia.

scholarly journals First Report of Onion yellow dwarf virus in Allium chinense in China

Plant Disease ◽  
2009 ◽  
Vol 93 (7) ◽  
pp. 761-761 ◽  
Author(s):  
C. C. Wu ◽  
N. Hong ◽  
Y. Liu ◽  
W. X. Xu ◽  
G. P. Wang
Keyword(s):  
Amino Acid Level ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Onion Yellow Dwarf Virus ◽  
Virus Species ◽  
First Report ◽  
Cp Gene ◽  
Virion Preparation ◽  
Yellow Dwarf Virus ◽  
Allium Chinense

Allium chinense (G. Don) is an economically important vegetable that has been considered to be of Asian origin (2). In April of 2007, plants of cultivated A. chinense showing mosaic, chlorotic streak, twist, and crinkle on leaves were collected from fields at Jiangxia, a suburban district of Wuhan, Hubei Province, China, and transplanted into pots in a greenhouse. Ultrathin sections of diseased leaves were observed under an electron microscope. Pinwheel or cylindrical inclusions, typical of a potyvirus infection, were observed. Filamentous virus particles (820 nm long) were also observed in the crude extract from the same plant. To identify the virus species, viral RNAs were extracted from partially purified virion preparation (3) and used as a template. First-strand cDNA was synthesized with M4-T (5′-GTT TTC CCA GTC ACG AC (T)15-3′) as a complementary primer. The 3′-terminus of viral RNA was amplified using primer M4 (5′-GTT TTC CCA GTC ACG AC-3′) in combination with a degenerate primer (5′-GGXAAYAAYAGY GGX CARCC-3′) that was specific for potyviruses (1). Amplified products were cloned and nine clones were sequenced. Sequence analysis showed that the size of the amplified fragment, excluding the poly A tail, was 1,625 bp, which had the typical characteristics of the 3′-terminus of the potyvirus genome, including the partial NIb gene (636 bp) and the complete coat protein (CP gene; 771 bp). The 1,625-bp sequence from A. chinense (Genbank Accession No. FJ765739) had the highest identity at the nucleotide level with sequences of Onion yellow dwarf virus (OYDV), ranging from 76% (Genbank Accession Nos. AB219833 and AB219834) to 99% (Genbank Accession No. AJ409313). The CP gene had 88% (Genbank Accession Nos. AB219833 and AB219834) to 99% (Genbank Accession Nos. AJ409313 and AJ409310) identity at the amino acid level with corresponding regions of known OYDV isolates from other hosts. Until now, only Scallion mosaic virus (ScaMV), in the genus Potyvirus, had been detected from A. chinense (1); however, OYDV was reported in rakkyo, the same species as Chinese scallion, in Japan (4). To our knowledge, this is the first report of OYDV infecting A. chinense in China. References: (1) J. Chen et al. Arch. Virol. 147:683, 2002. (2) L. K. Mann and W. T. Stearn. Econ. Bot. 14:69, 1960. (3) T. Nagakubo et al. Phytopathology 84:640,1994. (4) I. Sako et al. Ann. Phytopathol. Soc. Jpn. 57:65, 1991.

scholarly journals Detection of major viruses infecting shallot and molecular characterization of Onion yellow dwarf virus from several locations in Indonesia

2020 ◽  
Vol 21 (4) ◽  
Author(s):  
Heri Harti ◽  
SRI HENDRASTUTI HIDAYAT ◽  
SOBIR ◽  
SURYO WIYONO
Keyword(s):  
Molecular Characterization ◽  
Virus Detection ◽  
Latent Virus ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Onion Yellow Dwarf Virus ◽  
Dna Fragments ◽  
Field Samples ◽  
Yellow Dwarf Virus

Abstract. Harti H, Hidayat SH, Sobir, Wiyono S. 2020. Detection of major viruses infecting shallot and molecular characterization of Onion yellow dwarf virus from several locations in Indonesia. Biodiversitas 21: 1697-1701. Research was conducted to identify main viruses infecting shallot in several regions in Indonesia and to further characterize genetic variation of Onion yellow dwarf virus (OYDV). Field survey was conducted in Central Java (Brebes), East Java (Probolinggo), West Sumatera (Alahan Panjang), West Nusa Tenggara (Bima), and South Sulawesi (Enrekang). Virus detection from field samples was conducted by dot immunobinding assay. This detection confirmed that infection of OYDV, Shallot yellow stunt virus (SYSV), Shallot latent virus (SLV), and Garlic common latent virus (GarCLV) has occurred in all field with incidence ranged from 20 to 93.5%, 2 to 93%, 21.5 to 80%, and 2 to 80.5%, respectively. The specific primers of Nib gene successfully amplified DNA fragments of OYDV from all locations. Sequencing of DNA fragments revealed that the amplified product was 351 bp. Sequence analysis indicated that the present OYDV isolates from Indonesia shared homology from 82 to 95%; and they had homology from 81 to 95% with OYDV isolates from other countries. The similarity of OYDV isolates from different geographical locations reflected the movements of seed bulbs among and within countries. The phylogenetic tree also revealed that OYDV isolates from different countries did not group together indicating their diverse origin.

Onion yellow dwarf virus. [Distribution map].

2018 ◽  
Author(s):  
Keyword(s):  
New York ◽  
Far East ◽  
Uttar Pradesh ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Onion Yellow Dwarf Virus ◽  
Distribution Map ◽  
Yellow Dwarf Virus ◽  
Virus Distribution ◽  
Distribution In Europe

Abstract A new distribution map is provided for Onion yellow dwarf virus. Potyviridae: Potyvirus. Hosts: onion (Allium cepa) and garlic (Allium sativum). Information is given on the geographical distribution in Europe (Austria, Croatia, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Italy, mainland Italy, Sicily, Lithuania, Moldova, Netherlands, Poland, Portugal, Romania, Russia, Far East, Siberia, Serbia, Slovenia, Spain, UK, England and Wales and Ukraine), Asia (China, Henan, Hubei, Hunan, Jiangsu, Shandong, Yunnan, Zhejiang, India, Delhi, Gujarat, Haryana, Madhya Pradesh, Maharashtra, Indian Punjab, Rajasthan, Uttar Pradesh, Indonesia, Java, Iran, Israel, Japan, Pakistan, Taiwan, Turkey, Vietnam and Yemen), Africa (Egypt, Morocco, Nigeria and Sudan), North America (Canada, British Columbia, Nova Scotia, Ontario, Mexico, USA, California, Colorado, Florida, Idaho, Iowa, Minnesota, Nevada, New York, Oregon, Texas, Vermont, Washington and West Virginia), Central America and Caribbean (Cuba), South America (Argentina, Brazil, Bahia, Goias, Minas Gerais, Parana, Rio Grande do Sul, Santa Catarina, Sao Paulo, Chile, Ecuador and Urugay) and Oceania (Australia and New Zealand).

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