scholarly journals Necrotic streak disease of Chinese chive caused by Iris yellow spot virus (IYSV).

2007 ◽  
Vol 73 (4) ◽  
pp. 311-313 ◽  
Author(s):  
T. FUKUDA ◽  
K. NAKAYAMA ◽  
Y. HONDA
Keyword(s):  
Iris Yellow Spot Virus ◽  
Yellow Spot ◽  
Spot Virus ◽  
Chinese Chive

scholarly journals First Report of Iris yellow spot virus on Onion (Allium cepa) in Texas

Plant Disease ◽  
2006 ◽  
Vol 90 (10) ◽  
pp. 1359-1359 ◽  
Author(s):  
M. E. Miller ◽  
R. R. Saldana ◽  
M. C. Black ◽  
H. R. Pappu
Keyword(s):  
Disease Incidence ◽  
Iris Yellow Spot Virus ◽  
Yellow Spot ◽  
N Gene ◽  
Nucleotide Sequence Analysis ◽  
Rt Pcr ◽  
Onion Thrips ◽  
Spot Virus ◽  
Das Elisa ◽  
Bulb Yield

Iris yellow spot virus (IYSV; family Bunyaviridae, genus Tospovirus) has emerged as a potentially devastating and widespread virus of onion. IYSV was first reported in the United States from Idaho in 1993 and has since spread to many of the onion-producing areas (1). In South America, the most recent reports of the virus on onion were from Peru and Chile (2,4). In 2005, onion plants in Uvalde County, Texas exhibited necrotic lesions on leaves typical of IYSV and disease incidence approached 100% in some fields with yield loss and quality problems. Five of six plants tested were positive for IYSV with double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA; Agdia Inc., Elkhart, IN). In 2006, similar lesions were observed on onion plants in Uvalde County and approximately 400 km south in Hidalgo and Cameron counties. Infection points generally started as a single plant near the edge of fields and spread to plants in a 3- to 4-m area after 1 to 2 weeks. Early-season disease incidence was low in onions grown for bulbs and transplants, <10% in 2006. Disease incidence increased in some fields until the crop was harvested. Leaves of symptomatic plants were tested for IYSV and Tomato spotted wilt virus (TSWV) using DAS-ELISA, and 18 of 23 samples from the Hidalgo County area and 12 of 21 samples from the Uvalde County area were positive for IYSV. All samples tested for TSWV from these counties were negative. Virus infection in some ELISA-positive plants was verified by reverse transcription-polymerase chain reaction (RT-PCR) using primers derived from the small RNA of IYSV. The primers flanked the IYSV nucleocapsid (N) gene (5′-TAA AAC AAA CAT TCA AAC AA-3′ and 5′-CTC TTA AAC ACA TTT AAC AAG CAC-3′ (3). RT-PCR gave a PCR product of expected size (approximately 1.2 kb). The DNA amplicon was cloned and sequenced (GenBank Accession No. DQ658242). Nucleotide sequence analysis confirmed the identity of the amplicon as that of IYSV N gene and sequence comparisons with known IYSV N gene sequences showed 95 to 98% sequence identity. The primary vector of IYSV, onion thrips (Thrips tabaci), is a widespread and destructive pest of onion in south Texas. The year-to-year incidence of IYSV and the severity of the disease will probably depend on the onion thrips population levels. Bulb yield reduction could be severe during years with high thrips populations. More research is needed to determine the impact of IYSV on bulb yield in Texas, the relationship between IYSV incidence and T. tabaci population levels, and oversummering hosts. To our knowledge, this is the first known report of IYSV in Texas. References: (1) D. H. Gent et al. Plant Dis. 88:446, 2004, (2) S. W. Mullis et al. Plant Dis. 90:377, 2006, (3) H. Pappu et al. Arch. Virol. 151:1015, 2006. (4) M. Rosales et al. Plant Dis. 89:1245, 2005.

scholarly journals Iris yellow spot virus in Onion Seed Crops in South Africa

Plant Disease ◽  
2007 ◽  
Vol 91 (9) ◽  
pp. 1203-1203 ◽  
Author(s):  
L. J. du Toit ◽  
J. T. Burger ◽  
A. McLeod ◽  
M. Engelbrecht ◽  
A. Viljoen
Keyword(s):  
South Africa ◽  
Iris Yellow Spot Virus ◽  
Yellow Spot ◽  
Onion Bulb ◽  
Western Cape ◽  
Spot Virus ◽  
Total Rna ◽  
Onion Seed ◽  
Seed Crops ◽  
Np Gene

In December 2006, symptoms typical of iris yellow spot caused by Iris yellow spot virus (IYSV; genus Tospovirus, family Bunyaviridae) were observed on scapes (seed stalks) in an onion (Allium cepa L.) seed crop in the Klein Karoo of the Western Cape Province, South Africa. Symptoms included diamond-shaped chlorotic or necrotic lesions on the scapes, some of which had ‘green-islands’ with nested diamond-shaped lesions, as well as indistinct, circular to irregular, chlorotic or necrotic lesions of various sizes. At the time symptoms were observed, approximately 5% of the scapes had lodged as a result of extensive lesions resembling those caused by IYSV. The crop was 2 to 3 weeks from harvest. Symptomatic tissue from two plants (two samples from one plant and four samples from the other plant) was tested for IYSV by reverse-transcriptase (RT)-PCR. Total RNA was extracted from symptomatic scape tissue with the SV Total RNA Isolation System (Promega, Madison, WI) according to the manufacturer's instructions. First strand cDNA was synthesized with the RevertAid H Minus First Strand cDNA Synthesis kit (Fermentas Inc., Hanover, MD), followed by PCR amplification with primers IYSV-For (TGG YGG AGA TGY RGA TGT GGT) and IYSV-Rev (ATT YTT GGG TTT AGA AGA CTC ACC), which amplify the nucleocapsid (NP) gene of IYSV. An amplicon of expected size (approximately 750 bp) was observed for each of the symptomatic plants assayed and was sequenced. Comparison of the sequence (GenBank Accession No. EF579801) with GenBank sequences revealed 95% sequence identity with the NP gene of IYSV GenBank Accession No. EF419888, with eight amino acid differences. The known geographic distribution of IYSV in onion bulb or seed crops has increased rapidly in recent years in many areas of the world (1). To our knowledge, this is the first confirmation of IYSV in South Africa. Approximately 6,100 ha of onion bulb crops are grown annually in South Africa in the Western Cape, Kwazulu Natal, Limpopo, and Northern Cape provinces, and 600 ha of onion seed crops are grown primarily in the semi-arid regions of the Western Cape. Examination of an additional 10 onion seed crops in the Klein Karoo during January 2007 revealed the presence of iris yellow spot in three more crops at approximately 5% incidence in each crop. The four symptomatic crops had all been planted as bulb-to-seed crops, using vernalized bulbs produced on the same farm. This suggests that IYSV may have been disseminated into the seed crops on the vernalized bulbs, either as infected bulb tissue or in viruliferous thrips on the bulbs. Reference: (1) D. H. Gent et al. Plant Dis. 90:1468, 2006.

scholarly journals Variabilidad y síntomas causados por Iris yellow spot virus en Nicotiana benthamiana

2017 ◽  
Vol 35 (4) ◽  
Author(s):  
Katya Ornelas-Ocampo ◽  
Daniel Leobardo Ochoa-Martínez ◽  
Sergio Aranda-Ocampo ◽  
Sergio Ramírez-Rojas ◽  
Hernán García-Ruíz
Keyword(s):  
Allium Cepa ◽  
Nicotiana Benthamiana ◽  
Iris Yellow Spot Virus ◽  
Yellow Spot ◽  
Spot Virus

<p>En cultivos de cebolla <em>Allium cepa</em> del estado de Morelos, México, se observan síntomas típicos y severos asociados a <em>Iris yellow spot virus </em>(IYSV). En esta investigación se estudiaron las alteraciones que ocasionan los aislamientos de IYSV procedentes de síntomas típicos y severos en <em>Nicotiana benthamiana</em>, las diferencias en el gen N y su filogenia. Cuatro aislamientos típicos y cinco severos inoculados mecánicamente causaron infección sistémica. En cámara bioclimática los aislamientos severos ocasionaron mayor severidad de síntomas. La secuencia del gen N de ambos aislamientos tuvo 98-99% de identidad con la nucleoproteína de IYSV y no se observaron cambios en la secuencia de nucleótidos entre ellos. Ambos aislamientos se agruparon con el genotipo IYSV<sub>BR</sub> y tuvieron mayor similitud con los reportados en Canadá y Estados Unidos.</p>

scholarly journals Development of a sensitive Luminex xMAP-based microsphere immunoassay for specific detection of Iris yellow spot virus

2018 ◽  
Vol 15 (1) ◽  
Author(s):  
Cui Yu ◽  
Cuiyun Yang ◽  
Shaoyi Song ◽  
Zixiang Yu ◽  
Xueping Zhou ◽  
...  
Keyword(s):  
Iris Yellow Spot Virus ◽  
Yellow Spot ◽  
Specific Detection ◽  
Spot Virus ◽  
Microsphere Immunoassay

Iris yellow spot virus. [Distribution map].

2014 ◽  
Author(s):  
Keyword(s):  
New York ◽  
Tamil Nadu ◽  
Uttar Pradesh ◽  
Iris Yellow Spot Virus ◽  
Yellow Spot ◽  
Allium Porrum ◽  
Distribution Map ◽  
Spot Virus ◽  
South Wales ◽  
Virus Distribution

Abstract A new distribution map is provided for Iris yellow spot virus. Bunyaviridae: Tospovirus. Hosts: Onion (Allium cepa), Dutch iris (Iris hollandica), leek (Allium porrum) and garlic (Allium sativum). Information is given on the geographical distribution in Europe (Austria, Bosnia-Hercegovina, France, Germany, Greece, Italy, Netherlands, Serbia, Slovenia, Spain, UK, England and Wales), Asia (India, Gujarat, Karnataka, Madhya Pradesh, Maharashtra, Tamil Nadu, Uttar Pradesh, Indonesia, Java, Iran, Israel, Japan, Honshu, Kyushu, Pakistan, Sri Lanka and Tajikistan), Africa (Egypt, Kenya, Mauritius, Reunion, South Africa and Uganda), North America (Canada, Ontario, Mexico, USA, Arizona, California, Colorado, Georgia, Hawaii, Idaho, Michigan, Nevada, New Mexico, New York, Oregon, Pennsylvania, Texas, Utah and Washington), Central America and Caribbean (Guatemala), South America (Brazil, Pernambuco, Sao Paulo, Chile, Peru and Uruguay) and Oceania (Australia, New South Wales, Victoria, Western Australia and New Zealand).

scholarly journals Necrotic streaks of alstroemeria (Alstroemeria spp.) caused by Iris yellow spot virus (IYSV).

2005 ◽  
Vol 71 (2) ◽  
pp. 119-122 ◽  
Author(s):  
M. OKUDA ◽  
K. HANADA ◽  
S. UEMATSU ◽  
F. FUKUMOTO ◽  
T. MIHIRA ◽  
...  
Keyword(s):  
Iris Yellow Spot Virus ◽  
Yellow Spot ◽  
Spot Virus

Identification and incidence of Iris yellow spot virus in Allium field crops in Southwest Germany

2012 ◽  
Vol 134 (2) ◽  
pp. 345-356 ◽  
Author(s):  
H.-J. Krauthausen ◽  
Gabriele M. E. Leinhos ◽  
J. Müller ◽  
P. C. Radtke ◽  
J. A. Jehle
Keyword(s):  
Iris Yellow Spot Virus ◽  
Yellow Spot ◽  
Spot Virus ◽  
Field Crops ◽  
Southwest Germany

scholarly journals Molecular and Serological Characterization of Iris Yellow Spot Virus, a New and Distinct Tospovirus Species

1998 ◽  
Vol 88 (12) ◽  
pp. 1276-1282 ◽  
Author(s):  
I. Cortês ◽  
I. C. Livieratos ◽  
A. Derks ◽  
D. Peters ◽  
R. Kormelink
Keyword(s):  
Datura Stramonium ◽  
Distinct Species ◽  
Iris Yellow Spot Virus ◽  
Yellow Spot ◽  
Spot Virus ◽  
Serological Characterization ◽  
Tospovirus Species ◽  
Experimental Host Range

A new tospovirus was identified in iris cultivations in the Netherlands. Both serological comparisons and sequence determination of the S RNA demonstrate that this virus represents a new and distinct species, belonging to a separate serogroup, and for which the name iris yellow spot virus (IYSV) is proposed. The disease symptoms on iris are characterized by yellow spots on the leaves. Its experimental host range is very narrow and, in addition to iris, only includes Nicotiana benthamiana and Datura stramonium. The nucleoprotein of IYSV shows only 30 to 44% sequence identity with those of other tospoviruses identified so far; the highest homology being found with the tospovirus species of serogroup IV.

scholarly journals Spatial and Temporal Distribution of Iris yellow spot virus and Thrips in Colorado Onion Fields

2010 ◽  
Vol 11 (1) ◽  
pp. 11 ◽  
Author(s):  
Howard F. Schwartz ◽  
David H. Gent ◽  
Scott M. Fichtner ◽  
Rajiv Khosla ◽  
Linda A. Mahaffey ◽  
...  
Keyword(s):  
Soil Ph ◽  
Management Practices ◽  
Disease Incidence ◽  
Risk Index ◽  
Iris Yellow Spot Virus ◽  
Yellow Spot ◽  
Onion Thrips ◽  
Spot Virus ◽  
Negatively Associated ◽  
Sodium Percent

Iris yellow spot virus and its onion thrips vector (Thrips tabaci) are yield limiting pests of onion in the western United States. This two-year project investigated the relationship of iris yellow spot to thrips populations, soil properties, and grower management practices in six onion fields during 2005 and 2006 in Colorado. Thrips density (average number of thrips per plant per day) was negatively associated with soil pH (r = -0.61). The incidence of iris yellow spot at harvest was positively associated with organic matter, zinc, potassium, percent sodium, percent hydrogen, copper, iron, and soil pH. Thrips-days had a clear non-linear association with disease incidence at harvest. Onion plant population was positively associated with levels of iron, phosphorus, potassium, and zinc; and negatively associated with calcium, salts, percent potassium, and cation exchange capacity (CEC). Marketable (medium or larger sized bulbs) yield was negatively associated with phosphorus and percent potassium, and positively associated with salts, calcium, and CEC. Results from this project could contribute to the development of a risk index to predict potential risk from Iris yellow spot virus and its onion thrips vector. Accepted for publication 6 July 2010. Published 20 August 2010.

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