scholarly journals Leaf and tuber resistance to late blight in interspecific potato hybrids derived from Solanum kurtzianum

2018 ◽  
pp. 21-25
Author(s):  
E.M. Zoteyeva ◽  
Keyword(s):  
Late Blight ◽  
Tuber Resistance ◽  
Solanum Kurtzianum ◽  
Interspecific Potato Hybrids

scholarly journals Identification of Interspecific Potato Hybrids with Combined Resistance to Late Blight (Phytophthora Infestans) and Nematode (Globodera Rostochiensis)

2020 ◽  
Vol 74 (3) ◽  
pp. 188-195
Author(s):  
Nadezhda Zoteyeva ◽  
Guna Sprūde ◽  
Natalia Klimenko ◽  
Ieva Mežaka
Keyword(s):  
Molecular Markers ◽  
Late Blight ◽  
Phytophthora Infestans ◽  
Late Blight Resistance ◽  
Globodera Rostochiensis ◽  
Cyst Nematode ◽  
Potato Yields ◽  
Highly Correlated ◽  
Interspecific Potato Hybrids ◽  
Moderately Resistant

AbstractLate blight (agent Phytophthora infestans) and potato cyst nematode (PCN) caused by Globodera rostochiensis are economically important pathogens, which may significantly reduce potato yields. In this study interspecific potato hybrids were used as a source of resistance for combined resistance to economically important potato pathogens: late blight and cyst nematode. The aim of our study was to identify hybrid progenies with combined resistance to both pathogens and to verify the applicability of several molecular markers associated with resistance to G. rostochiensis pathotype Ro1 to identify resistant plants. Ninety-two clones of eleven original interspecific potato hybrids obtained in crosses with the cultivated S. tuberosum group tuberosum, S. tuberosum group Andigena, S. tuberosum group Phureja and wild S. guerreroense, S. microdontum, S. kurtzianum, S. neoantipoviczii and S. tarijense potato species were screened in bioassays and by molecular markers. PCN resistant or moderately resistant clones were found among the progenies of nine hybrids. Results were highly correlated with resistance status detected by molecular markers linked to the H1 (marker 57R) and Gro1-4 (marker Gro1) genes. Marker CP113 (linked to the H1 gene) was not polymorphic and failed to detect resistance status. Combination of foliar late blight resistance and resistance to PCN was identified in hybrids obtained in crosses with plants of species S. microdontum, S. tarijense and S. phureja and in the hybrid between S. guerreroense and Black’s P. infestans race differential carrying gene R-5.

Mapping polygenes for tuber resistance to late blight in a diploid Solanum phureja × S. stenotomum hybrid population

2006 ◽  
Vol 125 (4) ◽  
pp. 385-389 ◽  
Author(s):  
I. Simko ◽  
S. Costanzo ◽  
V. Ramanjulu ◽  
B. J. Christ ◽  
K. G. Haynes
Keyword(s):  
Late Blight ◽  
Solanum Phureja ◽  
Hybrid Population ◽  
Tuber Resistance

Evaluation of the RPi-ber late blight resistance gene for tuber resistance in the field and laboratory

2011 ◽  
Vol 130 (4) ◽  
pp. 464-468 ◽  
Author(s):  
Hilary Mayton ◽  
Gilda Rauscher ◽  
Ivan Simko ◽  
William E. Fry
Keyword(s):  
Late Blight ◽  
Resistance Gene ◽  
Late Blight Resistance ◽  
Blight Resistance ◽  
Tuber Resistance

Interspecific potato hybrids as a resource for late blight resistance genes

2014 ◽  
Vol 40 (1) ◽  
pp. 10-13
Author(s):  
E. V. Rogozina ◽  
V. A. Kolobaev ◽  
E. E. Khavkin ◽  
M. A. Kuznetsova ◽  
M. P. Beketova ◽  
...  
Keyword(s):  
Late Blight ◽  
Resistance Genes ◽  
Late Blight Resistance ◽  
Blight Resistance ◽  
Interspecific Potato Hybrids

scholarly journals Potato Tuber Blight Resistance Phenotypes Correlate with RB Transgene Transcript Levels in an Age-Dependent Manner

2015 ◽  
Vol 105 (8) ◽  
pp. 1131-1136 ◽  
Author(s):  
Benjamin P. Millett ◽  
Liangliang Gao ◽  
Massimo Iorizzo ◽  
Domenico Carputo ◽  
James M. Bradeen
Keyword(s):  
Late Blight ◽  
Late Blight Resistance ◽  
Blight Resistance ◽  
Dependent Manner ◽  
Transcript Levels ◽  
Tuber Resistance ◽  
Age Dependent ◽  
Organ Specific ◽  
Late Blight Disease ◽  
Blight Disease

Plants have evolved strategies and mechanisms to detect and respond to pathogen attack. Different organs of the same plant may be subjected to different environments (e.g., aboveground versus belowground) and pathogens with different lifestyles. Accordingly, plants commonly need to tailor defense strategies in an organ-specific manner. Phytophthora infestans, causal agent of potato late blight disease, infects both aboveground foliage and belowground tubers. We examined the efficacy of transgene RB (known for conferring foliar late blight resistance) in defending against tuber late blight disease. Our results indicate that the presence of the transgene has a positive yet only marginally significant effect on tuber disease resistance on average. However, a significant association between transgene transcript levels and tuber resistance was established for specific transformed lines in an age-dependent manner, with higher transcript levels indicating enhanced tuber resistance. Thus, RB has potential to function in both foliage and tuber to impart late blight resistance. Our data suggest that organ-specific resistance might result directly from transcriptional regulation of the resistance gene itself.

scholarly journals POPULATION STRUCTURE OF PHYTOPHTHORA INFESTANS ON WORLDWIDE SCALE: A REVIEW

2017 ◽  
Vol 29 (2) ◽  
pp. 281
Author(s):  
Khalid Naveed ◽  
Nasir A. Rajputt ◽  
Sajid A. Khan ◽  
Arbab Ahmad
Keyword(s):  
Population Structure ◽  
Late Blight ◽  
Phytophthora Infestans ◽  
Potato Cultivar ◽  
R Genes ◽  
Solanum Bulbocastanum ◽  
Tuber Resistance ◽  
Commercial Potato ◽  
Rb Gene ◽  
Late Blight Of Potato

Phytophthora infestans is a destructive pathogen that causes late blight of potato worldwide. Several sexually and asexually reproducing lineages of the pathogen have been identified and new lineages are more virulent as compared to their parental lineages. A new highly aggressive clonal lineage EU13_A2 has spread into potato fields of Europe, Africa and Asia in place of the older lineages. In North America, 24 clonal lineages US1to US24 have been identified. Despite of sexual reproduction, the overall population of P. infestans in potato and tomato fields is dominated by asexual lineages. Breeding has been done to transfer 'R' genes into commercial potato cultivars through classical breeding and by pyramiding of genes. Defender is the only potato cultivar that has foliar and tuber resistance to late blight. Genetically modified potato with RB gene from Solanum bulbocastanum has been developed but it lacks tuber resistance to disease. This review discusses population structure of P. infestans worldwide and breeding efforts to produce late blight resistant potato.

scholarly journals South American species Solanum alandiae Card. and S. okadae Hawkes et Hjerting as potential sources of genes for potato late blight resistance

2020 ◽  
Vol 181 (1) ◽  
pp. 73-83
Author(s):  
O. A. Muratova (Fadina) ◽  
M. P. Beketova ◽  
M. A. Kuznetsova ◽  
E. V. Rogozina ◽  
E. E. Khavkin
Keyword(s):  
Late Blight ◽  
Wild Species ◽  
Potato Cultivar ◽  
Late Blight Resistance ◽  
Potato Late Blight ◽  
South American ◽  
South American Species ◽  
Pests And Diseases ◽  
Sequence Characterized Amplified Region ◽  
Interspecific Potato Hybrids

For several decades, wild species of Solanum L. section Petota Dumort. have been involved in potato cultivar breeding for robust resistance to pests and diseases. Potato late blight (LB) is caused by oomycete Phytophthora infestans (Mont.) de Bary, and the genes for race-specific resistance to P. infestans (Rpi genes) have been introgressed into cultivated potatoes by remote crosses and trans- or cisgenesis, first from S. demissum Buk. and, more recently, from other wild species, such as S. bulbocastanum Dun., S. stoloniferum Schlechtd. et Bché, and S. venturii Hawkes et Hjerting (according to the nomenclature by Hawkes, 1990). Most wild species already involved in breeding for LB resistance came from North and Central Americas: series Bulbocastana (Rydb.) Hawkes, Demissa Buk. and Longipedicellata Buk., and some Rpi genes of these species have been already characterized in much detail. Rpi genes of South American species, including the series Tuberosa (Rydb.) Hawkes, have not been sufficiently investigated. Among the latter, this study focuses on the Rpi genes of S. alandiae Card. and S. okadae Hawkes et Hjerting. Four accessions of S. alandiae, one accession of S. okadae and 11 clones of interspecific potato hybrids comprising S. alandiae germplasm from the VIR collection were PCR-screened using specific SCAR (Sequence Characterized Amplified Region) markers for eight Rpi genes. SCAR amplicons of five Rpi genes registered in this study were validated by comparing their sequences with those of prototype genes deposited in the NCBI Genbank. Among the structural homologues of Rpi genes found in S. alandiae and S. okadae, of special interest are homologues of CC-NB-LRR resistance genes with broad specificity towards P. infestans races, in particular R2=Rpi-blb3, R8, R9a, Rpi-vnt1 and Rpi-blb2 (94–99, 94–99, 86–89, 92–98 and 91% identity with the prototype genes, respectively). Our data may help to better understand the process of Rpi gene divergence along with the evolution of tuberbearing Solanum species, particularly in the series Tuberosa.

scholarly journals The impact of R1and R3a genes on tuber resistance to late blight of the potato breeding clones

2016 ◽  
Vol 70 (2) ◽  
pp. 58-63
Author(s):  
Nadezhda Zoteyeva ◽  
Ilze Skrabule ◽  
Ieva Mežaka ◽  
Daiga Vilcāne ◽  
Guna Usele ◽  
...  
Keyword(s):  
Late Blight ◽  
Plant Material ◽  
Potato Breeding ◽  
Resistance Allele ◽  
Chain Reaction ◽  
Tuber Resistance ◽  
Significant Difference ◽  
Polymerase Chain ◽  
R1 Gene ◽  
The Impact

Abstract Potato breeding clones were evaluated for resistance to late blight (agent Phytophthora infestans) using tuber inoculation tests and for presence of the resistance alleles of R1 and R3a genes in polymerase chain reaction tests. Among clones tested those expressing high, moderate and low resistance were identified. The data were analysed for the impact of R1 and R3a genes on tuber resistance to late blight in tested plant material. In previous evaluations performed on smaller amount of clones the tuber resistance levels significantly depended on presence/absence of the resistance allele of R3a gene and did not depend on presence of R1 gene allele. In the current study the statistical analyses did not prove the significant difference in resistance levels depending on presence of the resistance alleles, neither of R1 gene, nor of R3a gene. Tuber resistant clones bearing R3a gene resistance alleles still noticeably prevailed over the clones bearing the alleles of R1 gene as well as over the clones bearing the no resistance alleles of both genes. In several cases the resistance of clones with detected resistance allele of R1 gene was higher compared to those derived from the same crosses and showing amplification of the allele of R3a gene or those with no resistance alleles. Clones accumulating the resistance alleles of both (R1 and R3a) genes expressed high tuber resistance accompanied by necrotic reaction.

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