A study of the effect of dipyridamole on erythrocyte deformability using an improved filtration technique

Applicability of the rock-bed filtration technique was investigated through pilot-plant experiments in Bangkok, Thailand. Polluted canal water was used as horizontal flow influent to two reactor channels filled with rocks. During one year operation, HRT, filter media, and aeration mode, were changed in several runs. The results showed that 1) the rock-bed filtration with aeration and the HRT more than 6 h can successfully improve polluted klong water by reducing the pollutants (e.g. 60-120mg/L of SS to 20-40 mg/L and 15-30 mg/L of BOD to 5-20 mg/L); 2) main removal mechanism seems to be the sedimentation resulting from the settleability enhanced by aeration, and the biofilm attached onto rocks also works in the reduction of soluble organic matter; 3) a combination of three rock sizes arranged in descending order showed best results; 4) longer HRT (13 h) produces better effluent but is not so effective if it exceeds 9 hours; 5) 60-70% of sediment IL was decomposed in a year, and porosity in rock beds reduced approximately 16%.

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Hereditary elliptocytosis with protein band 4.1 deficiency in the dog

Blood ◽

10.1182/blood.v61.2.373.373 ◽

1983 ◽

Vol 61 (2) ◽

pp. 373-377 ◽

Cited By ~ 18

Author(s):

JE Smith ◽

K Moore ◽

M Arens ◽

GA Rinderknecht ◽

A Ledet

Keyword(s):

Animal Model ◽

Membrane Protein ◽

Erythrocyte Membrane ◽

Protein Band ◽

Erythrocyte Deformability ◽

Membrane Stability ◽

Osmotic Sensitivity ◽

Hereditary Elliptocytosis ◽

Membrane Cytoskeleton ◽

Glutathione Deficiency

Abstract A dog with persistent elliptocytosis was studied. The dog had membrane protein band 4.1 deficiency, microcytosis, shortened erythrocyte lifespan, increased osmotic sensitivity, and a mild glutathione deficiency. Erythrocyte deformability and membrane stability were adversely effected. The dog's parents had decreased band 4.1, decreased stability, and some elliptocytosis. This disorder in dogs closely resembles human patients with band 4.1 deficiency and should provide a valuable animal model to study the erythrocyte membrane cytoskeleton.

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The Detection and Importance of Intravascular Platelet Aggregation in Surgical Shock

10.1055/s-0039-1687032 ◽

1979 ◽

Author(s):

C.N. McCollum

Keyword(s):

Femoral Vein ◽

Major Surgery ◽

Minor Surgery ◽

Post Operation ◽

Femoral Blood ◽

Shock Lung ◽

Platelet Aggregates ◽

Filtration Technique

Intravascular platelet aggregates (IPA) have as yet avoided detection in shocked patients hence their role in the aetiology of “shock lung” remains controversial. Screen filtration pressure (SFP) has only been shown to measure aggregates in vitro.A modified screen filtration technique was evaluated in 43 surgical patients. The characteristics (height,slope) of the pressure wave were compared with the number of aggregates seen to occlude filter pores on scanning electron microscopy (SEM). In 80 estimations on femoral vein blood the slope of the SFP curve was utilised improving SFP/ SEM correlation to r= .93. These aggregates arise in vivo as they were rarely detected in blood from the arm and IPA levels in femoral blood were not influenced by EDTA priming of the syringe. In 36 preoperative estimations mean SFP slope was identical to that in 14 patients after minor surgery (1.5 ± SD 1.6). After major surgery in 29 patients this value was elevated at 7.1 ± 6.1 (P<.001). Seventeen of these patients with SFP slope greater than 5 suffered a mean fall in arterial P2 at 5 days post operation of 1.85 KPa (13.9 mm Hg) which was significantly greater than that in the other major cases (0.85 KPa, 6.4 mm Hg)(P <.05). SFP also correlated closely with the fall in platelet count on day 1 post operation (r= .82, P< .001).Intravascular platelet aggregates arise in the veins of the lower limb immediately after major surgery. They can be measured by the screen filtration technique described and may be related to pulmonary dysfunction.

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