scholarly journals Campylobacter lari

2020 ◽  
Author(s):  
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2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Sarah Wigley ◽  
George M Garrity
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2013 ◽  
Vol 19 (2) ◽  
pp. 333-337 ◽  
Author(s):  
Shota Morishita ◽  
Hiromitsu Fujiwara ◽  
Hiromi Murota ◽  
Yumi Maeda ◽  
Ayako Hara ◽  
...  


2004 ◽  
Vol 54 (6) ◽  
pp. 2369-2373 ◽  
Author(s):  
Geoffrey Foster ◽  
Barry Holmes ◽  
Arnold G. Steigerwalt ◽  
Paul A. Lawson ◽  
Petra Thorne ◽  
...  

Phenotypic and phylogenetic studies were performed on four Campylobacter-like organisms recovered from three seals and a porpoise. Comparative 16S rRNA gene sequencing studies demonstrated that the organisms represent a hitherto unknown subline within the genus Campylobacter, associated with a subcluster containing Campylobacter jejuni, Campylobacter coli and Campylobacter lari. DNA–DNA hybridization studies confirmed that the bacteria belonged to a single species, for which the name Campylobacter insulaenigrae sp. nov. is proposed. The type strain of Campylobacter insulaenigrae sp. nov. is NCTC 12927T (=CCUG 48653T).



2017 ◽  
Author(s):  
Jennifer A. Schoborg ◽  
Jasmine Hershewe ◽  
Jessica C. Stark ◽  
Weston Kightlinger ◽  
James E. Kath ◽  
...  

AbstractProtein glycosylation, or the attachment of sugar moieties (glycans) to proteins, is important for protein stability, activity, and immunogenicity. However, understanding the roles and regulations of site-specific glycosylation events remains a significant challenge due to several technological limitations. These limitations include a lack of available tools for biochemical characterization of enzymes involved in glycosylation. A particular challenge is the synthesis of oligosaccharyltransferases (OSTs), which catalyze the attachment of glycans to specific amino acid residues in target proteins. The difficulty arises from the fact that canonical OSTs are large (>70 kDa) and possess multiple transmembrane helices, making them difficult to overexpress in living cells. Here, we address this challenge by establishing a bacterial cell-free protein synthesis platform that enables rapid production of a variety of OSTs in their active conformations. Specifically, by using lipid nanodiscs as cellular membrane mimics, we obtained yields of up to 440 µg/mL for the single-subunit OST enzyme, ‘Protein glycosylation B’ (PglB) from Campylobacter jejuni, as well as for three additional PglB homologs from Campylobacter coli, Campylobacter lari, and Desulfovibrio gigas. Importantly, all of these enzymes catalyzed N-glycosylation reactions in vitro with no purification or processing needed. Furthermore, we demonstrate the ability of cell-free synthesized OSTs to glycosylate multiple target proteins with varying N-glycosylation acceptor sequons. We anticipate that this broadly applicable production method will advance glycoengineering efforts by enabling preparative expression of membrane-embedded OSTs from all kingdoms of life.



2001 ◽  
Vol 7 (2) ◽  
pp. 96-97 ◽  
Author(s):  
M. Martinot ◽  
B. Jaulhac ◽  
R. Moog ◽  
S. De Martino ◽  
P. Kehrli ◽  
...  
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2006 ◽  
Vol 69 (1) ◽  
pp. 27-33 ◽  
Author(s):  
G. RASSCHAERT ◽  
K. HOUF ◽  
J. VAN HENDE ◽  
L. de ZUTTER

The relation between internal carriage and surface contamination with thermophilic Campylobacter species in broilers was examined by molecular typing methods. Samples from 39 flocks were collected in three Belgian poultry slaughterhouses. From each flock, crop swabs before slaughter and intestines and neck skins during slaughter were collected. A total of 309 isolates were identified at species level and further characterized by flagellin gene A PCR/restriction fragment length polymorphism and pulsed-field gel electrophoresis. Isolates were identified as Campylobacter jejuni (90%), Campylobacter coli (8.7%), and Campylobacter lari (2.2%), and 27 genotypes could be distinguished by combining the two molecular methods. Seventy-two percent of the flocks arriving at the abattoir were colonized with campylobacters. After slaughter, 79% of the flocks had contaminated neck skins. In six flocks, genotypes isolated from the neck skins were also found in the alimentary tract from previously slaughtered flocks. Four of these flocks were initially free of Campylobacter. These four flocks might have had no contaminated carcasses after logistic slaughtering.



2014 ◽  
Vol 71 (1) ◽  
pp. 19-28
Author(s):  
T. Nakajima ◽  
W. Ara ◽  
K. Saito ◽  
J.E. Moore ◽  
B.C. Millar ◽  
...  


1997 ◽  
Vol 34 (1) ◽  
pp. 79-88 ◽  
Author(s):  
Hubert P. Endtz ◽  
John S. Vliegenthart ◽  
Peter Vandamme ◽  
Hensley W. Weverink ◽  
Nicole P. van den Braak ◽  
...  


2006 ◽  
Vol 63 (1) ◽  
pp. 38-38
Author(s):  
H. Batori ◽  
T. Sekizuka ◽  
O. Murayama ◽  
J.E. Moore ◽  
B.C. Millar ◽  
...  


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