Sequence-specific Oligonucleotide Probe Polymerase Chain Reaction

2020 ◽  
Author(s):  
Keyword(s):  
Polymerase Chain Reaction ◽  
Oligonucleotide Probe ◽  
Chain Reaction ◽  
Specific Oligonucleotide Probe ◽  
Polymerase Chain ◽  
Sequence Specific Oligonucleotide Probe

scholarly journals Detection of residual leukemia by the polymerase chain reaction and sequence-specific oligonucleotide probe hybridization after allogeneic bone marrow transplantation for AKR leukemia: a murine model for minimal residual disease

Blood ◽  
1993 ◽  
Vol 81 (2) ◽  
pp. 551-559 ◽  
Author(s):  
WR Drobyski ◽  
LA Baxter-Lowe ◽  
RL Truitt
Keyword(s):  
Murine Model ◽  
Oligonucleotide Probe ◽  
Residual Disease ◽  
Allogeneic Bone Marrow Transplantation ◽  
Disease Recurrence ◽  
Allogeneic Bone ◽  
Specific Oligonucleotide Probe ◽  
Polymerase Chain ◽  
Probe Hybridization ◽  
Sequence Specific Oligonucleotide Probe

Disease relapse after allogeneic bone marrow transplantation (BMT) is a major cause of treatment failure and is thought to evolve from clinically occult residual disease in the recipient. However, the demonstration of minimal residual disease (MRD) in individual patients is of uncertain prognostic significance because the detection of residual disease has not consistently correlated with subsequent relapse. Moreover, the optimal therapeutic approach in patients with MRD after allogeneic BMT is unknown. The study of these issues has been hindered by the lack of clinically relevant animal models. In this report, we characterize a novel murine model for the study of MRD after allogeneic BMT. This model was designed to simulate high-risk BMT in humans in which patients receive transplants in relapse and disease recurrence in the major cause of treatment failure. The H-2-compatible, mixed lymphocyte culture nonreactive murine strains, AKR (H-2k) and CBA (H-2k), were chosen to parallel marrow transplants from HLA-matched siblings, which represent the majority of allo-transplants in humans. Male AKR leukemia cells were used in female donor/host chimeras permitting the Y chromosome to serve as a leukemia-specific marker for MRD. Detection of residual male leukemia cells in the peripheral blood of the primary host was facilitated by use of the polymerase chain reaction (PCR) and sequence-specific oligonucleotide probe hybridization (SSOPH). Use of PCR/SSOPH was highly predictive of clinical outcome (relapse or cure) in animals receiving transplants (P < .00002) and detected disease recurrence earlier than comparative flow cytometric analysis studies. This murine model will be useful in evaluating the efficacy of therapeutic strategies aimed at reducing disease relapse posttransplant and can be adapted to other transplant murine tumor systems for the study of MRD.

scholarly journals Detection of residual leukemia by the polymerase chain reaction and sequence-specific oligonucleotide probe hybridization after allogeneic bone marrow transplantation for AKR leukemia: a murine model for minimal residual disease

Blood ◽  
1993 ◽  
Vol 81 (2) ◽  
pp. 551-559 ◽  
Author(s):  
WR Drobyski ◽  
LA Baxter-Lowe ◽  
RL Truitt
Keyword(s):  
Murine Model ◽  
Oligonucleotide Probe ◽  
Residual Disease ◽  
Allogeneic Bone Marrow Transplantation ◽  
Disease Recurrence ◽  
Allogeneic Bone ◽  
Specific Oligonucleotide Probe ◽  
Polymerase Chain ◽  
Probe Hybridization ◽  
Sequence Specific Oligonucleotide Probe

Abstract Disease relapse after allogeneic bone marrow transplantation (BMT) is a major cause of treatment failure and is thought to evolve from clinically occult residual disease in the recipient. However, the demonstration of minimal residual disease (MRD) in individual patients is of uncertain prognostic significance because the detection of residual disease has not consistently correlated with subsequent relapse. Moreover, the optimal therapeutic approach in patients with MRD after allogeneic BMT is unknown. The study of these issues has been hindered by the lack of clinically relevant animal models. In this report, we characterize a novel murine model for the study of MRD after allogeneic BMT. This model was designed to simulate high-risk BMT in humans in which patients receive transplants in relapse and disease recurrence in the major cause of treatment failure. The H-2-compatible, mixed lymphocyte culture nonreactive murine strains, AKR (H-2k) and CBA (H-2k), were chosen to parallel marrow transplants from HLA-matched siblings, which represent the majority of allo-transplants in humans. Male AKR leukemia cells were used in female donor/host chimeras permitting the Y chromosome to serve as a leukemia-specific marker for MRD. Detection of residual male leukemia cells in the peripheral blood of the primary host was facilitated by use of the polymerase chain reaction (PCR) and sequence-specific oligonucleotide probe hybridization (SSOPH). Use of PCR/SSOPH was highly predictive of clinical outcome (relapse or cure) in animals receiving transplants (P < .00002) and detected disease recurrence earlier than comparative flow cytometric analysis studies. This murine model will be useful in evaluating the efficacy of therapeutic strategies aimed at reducing disease relapse posttransplant and can be adapted to other transplant murine tumor systems for the study of MRD.

Detection and haplotype differentiation of Southeast Asian α-thalassemia using polymerase chain reaction and a piezoelectric biosensor immobilized with a single oligonucleotide probe

2008 ◽  
Vol 151 (5) ◽  
pp. 246-254 ◽  
Author(s):  
Phantip Vattanaviboon ◽  
Kulphassorn Sangseekhiow ◽  
Pranee Winichagoon ◽  
Chamras Promptmas
Keyword(s):  
Polymerase Chain Reaction ◽  
Oligonucleotide Probe ◽  
Southeast Asian ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Polymerase chain reaction for salmonella virulence–associated plasmid genes detection: a new tool in salmonella epidemiology

1994 ◽  
Vol 112 (1) ◽  
pp. 33-43 ◽  
Author(s):  
L. Rexach ◽  
F. Dilasser ◽  
P. Fach
Keyword(s):  
Detection Method ◽  
Oligonucleotide Probe ◽  
Specific Response ◽  
Chain Reaction ◽  
Specific Oligonucleotide Probe ◽  
Salmonella Virulence ◽  
Polymerase Chain ◽  
Plasmid Genes ◽  
Standard Techniques

SummaryThe important role of plasmid genes in assessing virulence for BALB/c mice in salmonella, and the difficulty of using standard techniques to detect them, led us to develop a detection method by gene amplification.One hundred and forty–three strains (71 serovars) of salmonella and 35 strains of other species were tested using specific oligonucleotide primers. The amplification products were identified by a specific oligonucleotide probe. Forty-nine salmonella strains from ten serovars (S. abortus ovis, S. choleraesuis, S. dublin, S. enteritidis, S. gallinarum / pullorum, S. hessarek, S. typhimurium, S. IIIa48:z4, z23,S. IV43: z4, z23:-,S. V28:a:-) produced a positive and specific response.Because of various origins of the strains possessing the gene sought and the diversity of the responses, both from one serovar to another and in the same serovar, this search has its place among the epidemiological markers in general use. This method appears well suited to the research and detection of plasmid genes associated with mouse virulence in salmonella.

An alternative to DNA extraction for the diagnosis of Pneumocystis carinii pneumonia by polymerase chain reaction using a new oligonucleotide probe

1992 ◽  
Vol 6 (5) ◽  
pp. 361-365 ◽  
Author(s):  
Laurence Borensztein ◽  
Isabelle Hatin ◽  
Anne-Marie Simonpoli ◽  
Edgardo Ugarte ◽  
Pierre-Marie Girard ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Extraction ◽  
Pneumocystis Carinii Pneumonia ◽  
Oligonucleotide Probe ◽  
Pneumocystis Carinii ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Rapid identification ofAerococcus viridansusing the polymerase chain reaction and an oligonucleotide probe

1992 ◽  
Vol 95 (1) ◽  
pp. 63-69 ◽  
Author(s):  
Kathleen A. Grant ◽  
Joanne H. Dickinson ◽  
Matthew D. Collins ◽  
Rohan G. Kroll
Keyword(s):  
Polymerase Chain Reaction ◽  
Oligonucleotide Probe ◽  
Rapid Identification ◽  
Chain Reaction ◽  
Polymerase Chain
Sign in / Sign up

Export Citation Format

Share Document