Sarmad A. M. AL-Asadi Evaluation Of Fasciola gigantica Excretory-Secretory Antigen on Immunodiagnosis For Cattle Fascioliasis in AL-Chebayish Marshes By Using ELISA

The study was aimed at finding out the difference of protein content between excretory/secretory and somatic antigen of Fasciola gigantica as well as between excretory/secretory of Fasciola gigantica and Eurytrema pancreaticum. As many as 32 Fasciola gigantica and 30 Eurytrema pancreaticum were placed into 80 ml Roswell Park Memorial Institute (RPMI) 1640 and incubated for 6 hours. Excretory/secretory as well as somatic antigen was isolated and the concentration of protein was determined, using Lowry method. The result revealed the different of protein concentration. The concentrations of excretory/secretory antigen protein in Fasciola gigantica and Eurytrema pancreaticum were 3.386 mg/ml and 0.128, mg/ml respectively. The concentrations of somatic protein and Fasciola gigantica and Eurytrema pancreaticum were 8.028 mg/ml and 0.534 mg/ml, respectively. It can be concluded that the protein concentration from somatic tissue of Fasciola gigantica and Eurytrema pancreaticum is higher than the protein concentration from excretory/secretory of Fasciola gigantica and Eurytrema pancreaticum. The protein concentration from excerory/secretory antigen of Fasciola gigantica is higher than the protein concentration from excretory/secretory antigen of Eurytrema pancreaticum. ____________________________________________________________________________________________________________________ Key words: Fasciola gigantica, Eurytrema pancreaticum, protein concentation

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Serum and neutrophils alter the rate of excretory/secretory antigen release by Toxocara canis infective larvae in vitro

Parasite Immunology ◽

10.1111/j.1365-3024.1990.tb00946.x ◽

1990 ◽

Vol 12 (2) ◽

pp. 175-187 ◽

Cited By ~ 5

Author(s):

H.J.E. WILLIAMSON ◽

R.A. ALLARDYCE ◽

R.S. CLEMETT ◽

R.R. HIDAJAT

Keyword(s):

Toxocara Canis ◽

Infective Larvae ◽

Secretory Antigen ◽

Excretory Secretory Antigen ◽

Antigen Release

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DETECTION OF TOXOPLASMIC IMMUNITY BY MULTIPUNCTURE SKIN TEST WITH EXCRETORY-SECRETORY ANTIGEN

The Lancet ◽

10.1016/s0140-6736(85)90226-0 ◽

1985 ◽

Vol 326 (8447) ◽

pp. 121-123 ◽

Cited By ~ 8

Author(s):

D ROUGIER

Keyword(s):

Skin Test ◽

Secretory Antigen ◽

Excretory Secretory Antigen

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Juvenile female Litomosoides sigmodontis produce an excretory/secretory antigen (Juv-p120) highly modified with dimethylaminoethanol

Parasitology ◽

10.1017/s0031182098002996 ◽

1998 ◽

Vol 117 (3) ◽

pp. 265-271 ◽

Cited By ~ 11

Author(s):

M. HINTZ ◽

G. SCHARES ◽

A. TAUBERT ◽

R. GEYER ◽

H. ZAHNER ◽

...

Keyword(s):

Juvenile Female ◽

Litomosoides Sigmodontis ◽

Secretory Antigen ◽

Excretory Secretory Antigen

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Serological evidence of toxocariasis in patients from Spain with a clinical suspicion of visceral larva migrans

Journal of Helminthology ◽

10.1017/s0022149x00000729 ◽

1997 ◽

Vol 71 (1) ◽

pp. 9-12 ◽

Cited By ~ 15

Author(s):

S. Fenoy ◽

C. Cuéllar ◽

J.L. Guillén

Keyword(s):

Urban Areas ◽

Clinical Suspicion ◽

Larva Migrans ◽

Visceral Larva Migrans ◽

Elisa Method ◽

Serological Evidence ◽

Rural And Urban Areas ◽

Rural And Urban ◽

Secretory Antigen ◽

Excretory Secretory Antigen

AbstractSera from patients with clinical characteristics of toxocariasis were assayed using the ELISA method and larval excretory-secretory antigen. Four hundred and seven samples of Toxocara serology were received at the laboratory of ‘Ciudad Sanitaria Juan Canalejo’ Hospital of Corunna, Spain, from 1984 to 1989. Of these, 30 were from adults, 332 from children and 45 from patients of unknown age, resulting in Toxocara seroprevalences of 23.3%, 32.8% and 17.7% respectively. The reasons for these serological differences in the rural and urban areas of Galicia, Spain are discussed.

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