scholarly journals 15. KONSENTRASI PROTEIN ANTIGEN EKSKRETORI/SEKRETORI DAN SOMATIK PADA Fasciola gigantica DAN Eurytrema pancreaticum The Protein Concentration of Excretory/Secretory and Somatic Antigen from Fasciola gigantica and Eurytrema pancreaticum

2016 ◽  
Vol 10 (2) ◽  
Author(s):  
Muhammad Hambal
Keyword(s):  
Protein Concentration ◽  
Somatic Tissue ◽  
Roswell Park Memorial Institute ◽  
Fasciola Gigantica ◽  
Somatic Antigen ◽  
Secretory Antigen ◽  
Lowry Method ◽  
Antigen Protein ◽  
The Difference ◽  
Excretory Secretory Antigen

The study was aimed at finding out the difference of protein content between excretory/secretory and somatic antigen of Fasciola gigantica as well as between excretory/secretory of Fasciola gigantica and Eurytrema pancreaticum. As many as 32 Fasciola gigantica and 30 Eurytrema pancreaticum were placed into 80 ml Roswell Park Memorial Institute (RPMI) 1640 and incubated for 6 hours. Excretory/secretory as well as somatic antigen was isolated and the concentration of protein was determined, using Lowry method. The result revealed the different of protein concentration. The concentrations of excretory/secretory antigen protein in Fasciola gigantica and Eurytrema pancreaticum were 3.386 mg/ml and 0.128, mg/ml respectively. The concentrations of somatic protein and Fasciola gigantica and Eurytrema pancreaticum were 8.028 mg/ml and 0.534 mg/ml, respectively. It can be concluded that the protein concentration from somatic tissue of Fasciola gigantica and Eurytrema pancreaticum is higher than the protein concentration from excretory/secretory of Fasciola gigantica and Eurytrema pancreaticum. The protein concentration from excerory/secretory antigen of Fasciola gigantica is higher than the protein concentration from excretory/secretory antigen of Eurytrema pancreaticum. ____________________________________________________________________________________________________________________ Key words: Fasciola gigantica, Eurytrema pancreaticum, protein concentation

Characterisation of Fasciola gigantica adult 27-kDa excretory-secretory antigen in human fascioliasis

2003 ◽  
Vol 91 (4) ◽  
pp. 325-327 ◽  
Author(s):  
C. Tantrawatpan ◽  
W. Maleewong ◽  
C. Wongkham ◽  
S. Wongkham ◽  
P. M. Intapan ◽  
...  
Keyword(s):  
Fasciola Gigantica ◽  
Human Fascioliasis ◽  
Secretory Antigen ◽  
Excretory Secretory Antigen

DEVELOPMENT AND YIELD OF CANADIAN WESTERN RED SPRING AND CANADA PRAIRIE SPRING WHEATS AS AFFECTED BY DELAYED SEEDING IN THE BROWN AND DARK BROWN SOIL ZONES OF SASKATCHEWAN

1990 ◽  
Vol 70 (3) ◽  
pp. 639-660 ◽  
Author(s):  
H. W. CUTFORTH ◽  
C. A. CAMPBELL ◽  
D. JUDIESCH ◽  
R. M. DePAUW ◽  
J. M. CLARKE ◽  
...  
Keyword(s):  
Protein Concentration ◽  
Grain Quality ◽  
Frost Damage ◽  
Wheat Cultivars ◽  
Brown Soil ◽  
Soil Zone ◽  
Seeding Date ◽  
Optimum Period ◽  
Early Fall ◽  
The Difference

Historically, research has identified the optimum period of seeding Canadian Western Red Spring (CWRS) wheat in the Brown soil zone to be between late April and mid-May, and approximately mid-May in the Dark Brown soil zone. The recent development and release of new spring wheat cultivars with significantly different genetic makeup (Canada Prairie Spring (CPS) wheats) prompted this study to determine if the established criteria were valid for them. Seeding date experiments involving one CWRS wheat (Neepawa) and three CPS wheats (HY320, HY355, HY912) were carried out at Swift Current (1984–1988) and at Regina and Scott (1985–1988). Although significant seeding date × cultivar interactions occurred for several of the characteristics measured (e.g., yield, protein concentration), no cultivar consistently responded differently than the others. However, the difference in days to reach maturity between the later maturing CPS cultivars and the CWRS cultivar, Neepawa, generally increased with delayed seeding making the CPS cultivars more susceptible to early fall frost damage which could decrease their yields and grain quality. Our results showed no reason to change recommendations for seeding dates in either soil zone, and both types of wheat can be treated similarly except that late seeding for CPS wheats should be avoided.Key words: Seeding date, grain yield, GDD, protein, wheat

Juvenile female Litomosoides sigmodontis produce an excretory/secretory antigen (Juv-p120) highly modified with dimethylaminoethanol

Parasitology ◽  
1998 ◽  
Vol 117 (3) ◽  
pp. 265-271 ◽  
Author(s):  
M. HINTZ ◽  
G. SCHARES ◽  
A. TAUBERT ◽  
R. GEYER ◽  
H. ZAHNER ◽  
...  
Keyword(s):  
Juvenile Female ◽  
Litomosoides Sigmodontis ◽  
Secretory Antigen ◽  
Excretory Secretory Antigen

Filtration coefficient in cat hindlimb using protein concentration changes

1989 ◽  
Vol 256 (1) ◽  
pp. H186-H194 ◽  
Author(s):  
P. D. Watson ◽  
M. B. Wolf
Keyword(s):  
Protein Concentration ◽  
Venous Pressure ◽  
Filtration Coefficient ◽  
Volume Changes ◽  
Vascular Volume ◽  
Perfusate Flow ◽  
Wide Range ◽  
Concentration Changes ◽  
The Difference ◽  
Pentobarbital Sodium Anesthesia

The maximum value of capillary filtration coefficient (CFC) in maximally vasodilated cat skeletal muscle is disputed. It was hypothesized that the wide range of reported values was caused by the inability of gravimetric and volumetric measurements of tissue volume to separate transcapillary filtration from vascular volume changes. Consequently, we developed a method of measuring filtration rates from changes in venous protein concentration using Evan's blue-labeled albumin in the isolated hindlimb (pentobarbital sodium anesthesia). The filtration coefficient (PFFC) calculated from these filtration rates after a step in venous pressure should not be influenced by vascular volume changes. When the perfusate flow rate through the hindlimb was greater than 15 ml.min-1.100 g muscle-1, PFFC was 0.0085 +/- 0.0015 (SD, n = 8) ml.min-1.mmHg-1.100 g muscle-1. PFFC was observed to be unvarying from 1 to 12 min after the venous pressure elevation, in contrast to CFC values, which fall during the same period. It is argued that the difference between CFC and PFFC values is caused by vascular volume changes.

Serological evidence of toxocariasis in patients from Spain with a clinical suspicion of visceral larva migrans

1997 ◽  
Vol 71 (1) ◽  
pp. 9-12 ◽  
Author(s):  
S. Fenoy ◽  
C. Cuéllar ◽  
J.L. Guillén
Keyword(s):  
Urban Areas ◽  
Clinical Suspicion ◽  
Larva Migrans ◽  
Visceral Larva Migrans ◽  
Elisa Method ◽  
Serological Evidence ◽  
Rural And Urban Areas ◽  
Rural And Urban ◽  
Secretory Antigen ◽  
Excretory Secretory Antigen

AbstractSera from patients with clinical characteristics of toxocariasis were assayed using the ELISA method and larval excretory-secretory antigen. Four hundred and seven samples of Toxocara serology were received at the laboratory of ‘Ciudad Sanitaria Juan Canalejo’ Hospital of Corunna, Spain, from 1984 to 1989. Of these, 30 were from adults, 332 from children and 45 from patients of unknown age, resulting in Toxocara seroprevalences of 23.3%, 32.8% and 17.7% respectively. The reasons for these serological differences in the rural and urban areas of Galicia, Spain are discussed.

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