Biodegradation of 2,4,6-Trinitrotoluene (TNT) with Bacteria Isolated from TNT-polluted Waste Pink Water

In this study, bacterial strains that can use TNT as a nitrogen source isolated from TNT contaminated pink water. We isolated 5 bacterial strains and the isolated bacteria were cultured in medium containing TNT and TNT degradation capacities of isolates were determined by spectrophotometric analysis. According to the results of the analysis that have done, 3 bacterial isolates that have high TNT degradation capacity were selected and the isolates were identified with firstly Gram-staining then with 16S rRNA sequence analysis method. According to the sequence of 16S rRNA, water isolates were identified as Stenotrophomonas maltophilia (SU K2), Klebsiella pneumoniae (SU K3), Raoultella planticola (SU K4). During the TNT degradation studies, at the end of 24 h incubation time, in the medium containing 100 mg/L TNT, TNT degradation rate for SU K2, SU K3 and SU K4 were determined 70 %, 96 % and 93 % respectively. 4-aminodinitrotoluene and 2-aminodinitrotoluene accumulations were detected in the culture medium of all isolates as intermediate products formed during the degradation of TNT by HPLC analysis. Additionally, nitrite accumulation was detected in the culture medium of all isolates and the influence of temperature and pH on the degradation of TNT was also investigated. It was determined that SU K2 isolates have the highest TNT degradation capacity at 35 °C, the others have at 30 °C and all isolates degraded TNT fastest at pH 7. The results of the study show that the new isolates can be useful for the removal of TNT in a wastewater treatment system.

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Mycobacterium neoaurumBacteremia in a Hemodialysis Patient

Canadian Journal of Infectious Diseases ◽

10.1155/2003/840103 ◽

2003 ◽

Vol 14 (1) ◽

pp. 45-48 ◽

Cited By ~ 8

Author(s):

Marissa L Becker ◽

Amar A Suchak ◽

Joyce N Wolfe ◽

Ryan Zarychanski ◽

Amin Kabani ◽

...

Keyword(s):

Sequence Analysis ◽

16S Rrna ◽

Hemodialysis Patient ◽

Immunocompromised Host ◽

Blood Cultures ◽

Clinical Suspicion ◽

Diabetic Woman ◽

Rrna Sequence ◽

16S Rrna Sequence ◽

16S Rrna Sequence Analysis

Bacteremia due toMycobacterium neoaurum, a rapidly growing mycobacterium, is described in a diabetic woman on hemodialysis. This is the first reported case of M neoaurum bacteremia in Canada. The organism initially grew on standard BacT/Alert SA aerobic blood cultures, and was subsequently positively identified using 16S rRNA sequence analysis. The present case serves to reinforce the need for a high index of clinical suspicion of infections caused by unusual microorganisms in the context of an immunocompromised host.

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ISOLATION AND CHARACTERIZATION OF BIOTECHNOLOGY RELEVANT BACTERIA FROM MARINE ENVIRONMENT

Jurnal Teknologi ◽

10.11113/jt.v77.6893 ◽

2015 ◽

Vol 77 (31) ◽

Author(s):

Suganthi Thevarajoo ◽

Chitra Selvaratnam ◽

Kian Mau Goh ◽

Fazilah Abd. Manan ◽

Zaharah Ibrahim ◽

...

Keyword(s):

16S Rrna ◽

Marine Environment ◽

Antibiotic Sensitivity ◽

Marine Microorganisms ◽

Bacterial Strains ◽

Biochemical Tests ◽

16S Rrna Sequence ◽

Gram Staining ◽

Isolation And Characterization

Marine environment remained as largely unexplored source for researchers to discover marine microorganisms with novel properties. This study aims to isolate marine bacteria from the seashore of Desaru, Malaysia. Totally, six bacterial strains were successfully obtained and were identified by complete 16S rRNA sequencing. The characterizations of bacterial strains were performed based on morphological tests, Gram-staining, biochemical tests, and antibiotic sensitivity. The 16S rRNA sequence of D-2, D-4, D-7, D-15, D-31, and D-33 revealed a high identity of 97 to 99% with taxa belong to genera of Pseudomonas, Marinomonas, Exiquobacterium, Micrococcus, Pseudoalteromonas, and Shewanella respectively. Strain D-31 exhibited higher tolerance towards antibiotics kanamycin, ampicillin, and erythromycin while the growth of other strains were retarded by at least two of these antibiotics. We further characterized strain D-4 and D-31 that belonged to Marinomonas sp. and Pseudoalteromonas sp.. Both genera are interesting as earlier researchers have discovered new antibacterial substances, industrial enzymes and unique secondary metabolites.

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Mycobacterium marinum infections in humans and tracing of its possible environmental sources

Canadian Journal of Microbiology ◽

10.1139/w11-104 ◽

2012 ◽

Vol 58 (1) ◽

pp. 39-44 ◽

Cited By ~ 13

Author(s):

Michal Slany ◽

Petr Jezek ◽

Vera Fiserova ◽

Monika Bodnarova ◽

Jiri Stork ◽

...

Keyword(s):

Sequence Analysis ◽

16S Rrna ◽

Correct Diagnosis ◽

Low Frequency ◽

Mycobacterium Marinum ◽

Mycobacterium Fortuitum ◽

Rrna Sequence ◽

16S Rrna Sequence ◽

Mycobacterium Chelonae ◽

16S Rrna Sequence Analysis

The low frequency of nontuberculous mycobacterial infections, nonspecific symptoms for individual mycobacteria, and the lack of specific identification methods could alter correct diagnosis. This study presents a combined microbiology and molecular-based approach for Mycobacterium marinum detection in four aquarists with cutaneous mycobacterial infection. Simultaneously, ecology screening for M. marinum presence in the aquarists’ fish tanks was performed. A total of 38 mycobacterial isolates originated from four human patients (n = 20), aquarium animals (n = 8), and an aquarium environment (n = 10). Isolate identification was carried out using 16S rRNA sequence analysis. A microbiology-based approach, followed by 16S rRNA sequence analysis, was successfully used for detection of M. marinum in all four patients. Animal and environmental samples were simultaneously examined, and a total of seven mycobacterial species were isolated: Mycobacterium chelonae , Mycobacterium fortuitum , Mycobacterium gordonae , Mycobacterium kansasii , Mycobacterium mantenii , Mycobacterium marinum , and Mycobacterium peregrinum . The presence of M. marinum was proven in the aquarium environments of two patients. Although M. marinum is described as being present in water, it was detected only in fish.

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Phylogenetic structure of the genera Flexibacter, Flexithrix, and Microscilla deduced from 16S rRNA sequence analysis.

The Journal of General and Applied Microbiology ◽

10.2323/jgam.48.155 ◽

2002 ◽

Vol 48 (3) ◽

pp. 155-165 ◽

Cited By ~ 66

Author(s):

Yasuyoshi Nakagawa ◽

Takeshi Sakane ◽

Makoto Suzuki ◽

Kazunori Hatano

Keyword(s):

Sequence Analysis ◽

16S Rrna ◽

Rrna Sequence ◽

16S Rrna Sequence ◽

Phylogenetic Structure ◽

16S Rrna Sequence Analysis

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Subcuticular Bacteria Associated With Two Common New Zealand Echinoderms: Characterization Using 16S rRNA Sequence Analysis and Fluorescencein situHybridization

Biological Bulletin ◽

10.1086/bblv218n1p95 ◽

2010 ◽

Vol 218 (1) ◽

pp. 95-104 ◽

Cited By ~ 7

Author(s):

Scott A. Lawrence ◽

Ronan O'Toole ◽

Michael W. Taylor ◽

Simon K. Davy

Keyword(s):

New Zealand ◽

Sequence Analysis ◽

16S Rrna ◽

Rrna Sequence ◽

16S Rrna Sequence ◽

16S Rrna Sequence Analysis

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Cleaning and Handling Implements as Potential Reservoirs for Bacterial Contamination of Some Ready-to-Eat Foods in Retail Delicatessen Environments

Journal of Food Protection ◽

10.4315/0362-028x-70.12.2878 ◽

2007 ◽

Vol 70 (12) ◽

pp. 2878-2883 ◽

Cited By ~ 16

Author(s):

C. A. CHRISTISON ◽

D. LINDSAY ◽

A. von HOLY

Keyword(s):

South Africa ◽

Sequence Analysis ◽

16S Rrna ◽

Rrna Sequence ◽

Gram Positive ◽

16S Rrna Sequence ◽

Bacterial Populations ◽

16S Rrna Sequence Analysis ◽

And Staphylococcus Aureus ◽

Disposable Plastic

This study assessed the association of bacteria with cleaning tools, such as floor mops (n = 25) and cleaning cloths (n = 39), and handling devices, such as disposable plastic gloves (n = 20), used during filled baguette and assorted salad preparation in four selected retail delicatessens in Johannesburg, South Africa. Samples of each cleaning or handling tool were prepared for aerobic (APC), coliform (CC), Escherichia coli (EC), Bacillus cereus (BCC), and Staphylococcus aureus (SAC) counts, as well as tested for the incidence of Listeria monocytogenes (LM) and Salmonella (SALM) by standard plating methods. Bacterial populations attached to the cleaning and handling tools were observed by scanning electron microscopy (SEM). Ten selected gram-positive isolates were further analyzed by 16S rRNA sequence analysis and compared with isolates from filled baguettes and assorted salads. The floor mops consistently yielded the highest APCs, CCs, and ECs (5.7, 4.1, and 3.0 log CFU/g, respectively), while gloves had the lowest corresponding counts (3.6, 2.0, and 1.0 log CFU/g, respectively). Low BCCs and SACs were recorded in this study (ca. 1.2 log CFU/g), while SALM and LM were each detected in five cleaning tool samples. SEM showed rods and cocci attached to handling and cleaning tools. Furthermore, results of 16S rRNA sequence analysis indicated that several gram-positive isolates were identified as S. aureus, Staphylococcus pasteuri, Staphylococcus sciuri, and Enterococcus faecalis. Genetically similar strains (100% similarity) were isolated from cleaning and handling tools and associated ready-to-eat (RTE) foods. Cleaning and handling tools may act as reservoirs of contamination for RTE foods during preparation in retail delicatessens in South Africa. The transfer of potential pathogens, such as S. aureus, to foods from cleaning and handling tools may hold food safety implications.

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New insights into Siboglinidae microbiota - external tube contributes to an increment of the total microbial biomass

10.7287/peerj.preprints.27730 ◽

2019 ◽

Author(s):

Blanca Rincón-Tomás ◽

Luis Somoza ◽

Kathrin Sauter ◽

Dorothea Hause-Reitner ◽

Pedro Madureira ◽

...

Keyword(s):

Electron Microscopy ◽

Microbial Biomass ◽

16S Rrna ◽

Cold Seeps ◽

Rrna Sequence ◽

16S Rrna Sequence ◽

Transmission Electron ◽

Elemental Analyses ◽

16S Rrna Sequence Analysis ◽

El Cid

Siboglinid worms were sampled from four mud volcanoes in the Gulf of Cádiz (El Cid MV, Bonjardim MV, Al Gacel MV and Anastasya MV). These invertebrates are characteristic to cold seeps and are known to host chemosynthetic endosymbionts in a dedicated trophosome organ. However, little is known about their tube as a potential niche for other chemosynthetic and non-chemosynthetic microorganisms. Analyses by scanning and transmission electron microscopy showed dense biofilms on the tube in Al Gacel MV and Anastasya MV specimens by prokaryotic cells. Methanotrophic bacteria were the most abundant forming these biofilms as further confirmed by 16S rRNA sequence analysis. Furthermore, elemental analyses with electron microscopy and EDX point to the progressive mineralization of the biofilm and the tube in absence of nutrients. Environmental bacterial and archaeal 16S rRNA sequence libraries revealed abundant microorganisms related to these siboglinid worms and variation in microbial communities among samples. We argue that these differences must be related to variance in seepage activity, as it is the main source of nutrients. Thus, the tube remarkably increases the microbial biomass related to the worms and needs to be incorporated as an important part of the worm’s microbiota. Furthermore, empty tubes may still influence the composition of the active microbial community at those sites.

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