scholarly journals Ethanol extract of Artemisia sieversiana exhibits anticancer effects and induces apoptosis through a mitochondrial pathway involving DNA damage in COLO-205 colon carcinoma cells

2015 ◽  
Vol 10 (3) ◽  
pp. 518 ◽  
Author(s):  
Jun Tang ◽  
Juan-Juan Zhao ◽  
Zhi-Hong Li
Keyword(s):  
Colon Cancer ◽  
Dna Damage ◽  
Membrane Potential ◽  
Mitochondrial Membrane Potential ◽  
Cell Lines ◽  
Mitochondrial Membrane ◽  
Herbal Extract ◽  
Potential Loss ◽  
Mitochondrial Membrane Potential Loss ◽  
Ht 29

<p>The aim of the study was to see the antiproliferative and apoptotic effects of ethanolic herbal extract of Artemisia sieversiana against three human colon cancer (HT-29, HCT-15 and COLO-205) cells. The cytotoxicity of the extract on these cell lines was evaluated by MTT assay. Phase contrast and fluorescence microscopy using acridine orange/ethidium bromide (AO/ETBR) staining was employed to investigate morphological alterations in COLO-205 cells by the herbal extract. Flow cytometry instrument measured the changes in mitochondrial membrane potential loss while as gel electrophoresis measured DNA damage in these cells. The extract at increasing doses exhibited a strong cytotoxic effect in a dose-dependent manner against all the three colon cancer cell lines. The IC<sub>50</sub> values of the extract against HT-29, HCT-15 and COLO-205 cancer cells were found to be 52.1, 43.2 and 38.6 µg/mL respectively. Mitochondrial membrane potential loss (ΔΨm) and DNA fragmentation events were also observed following extract treatment at increasing doses.  </p><p> </p>

scholarly journals Rhomboid Protease PARL Mediates the Mitochondrial Membrane Potential Loss-induced Cleavage of PGAM5

2012 ◽  
Vol 287 (41) ◽  
pp. 34635-34645 ◽  
Author(s):  
Shiori Sekine ◽  
Yusuke Kanamaru ◽  
Masato Koike ◽  
Ayako Nishihara ◽  
Masahiro Okada ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Rhomboid Protease ◽  
Potential Loss ◽  
Mitochondrial Membrane Potential Loss

scholarly journals Mitochondrial Complex II Prevents Hypoxic but Not Calcium- and Proapoptotic Bcl-2 Protein-induced Mitochondrial Membrane Potential Loss

2010 ◽  
Vol 285 (34) ◽  
pp. 26494-26505 ◽  
Author(s):  
Brian J. Hawkins ◽  
Mark D. Levin ◽  
Patrick J. Doonan ◽  
Nataliya B. Petrenko ◽  
Christiana W. Davis ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Mitochondrial Complex ◽  
Complex Ii ◽  
Potential Loss ◽  
Mitochondrial Membrane Potential Loss

scholarly journals The Myxoma Poxvirus Protein, M11L, Prevents Apoptosis by Direct Interaction with the Mitochondrial Permeability Transition Pore

2002 ◽  
Vol 196 (9) ◽  
pp. 1127-1140 ◽  
Author(s):  
Helen Everett ◽  
Michele Barry ◽  
Xuejun Sun ◽  
Siow Fong Lee ◽  
Christine Frantz ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Mitochondrial Permeability Transition Pore ◽  
Mitochondrial Permeability Transition ◽  
Permeability Transition Pore ◽  
Permeability Transition ◽  
Mitochondrial Permeability ◽  
Potential Loss ◽  
Mitochondrial Membrane Potential Loss

M11L, an antiapoptotic protein essential for the virulence of the myxoma poxvirus, is targeted to mitochondria and prevents the loss of mitochondrial membrane potential that accompanies cell death. In this study we show, using a cross-linking approach, that M11L physically associates with the mitochondrial peripheral benzodiazepine receptor (PBR) component of the permeability transition (PT) pore. Close association of M11L and the PBR is also indicated by fluorescence resonance energy transfer (FRET) analysis. Stable expression of M11L prevents the release of mitochondrial cytochrome c induced by staurosporine or protoporphyrin IX (PPIX), a ligand of the PBR. Transiently expressed M11L also prevents mitochondrial membrane potential loss induced by PPIX, or induced by staurosporine in combination with PK11195, another ligand of the PBR. Myxoma virus infection and the associated expression of early proteins, including M11L, protects cells from staurosporine- and Fas-mediated mitochondrial membrane potential loss and this effect is augmented by the presence of PBR. We conclude that M11L regulates the mitochondrial permeability transition pore complex, most likely by direct modulation of the PBR.

scholarly journals Gelsolin Inhibits Apoptosis by Blocking Mitochondrial Membrane Potential Loss and CytochromecRelease

2000 ◽  
Vol 275 (20) ◽  
pp. 15343-15349 ◽  
Author(s):  
Richard Chikara Koya ◽  
Hisakazu Fujita ◽  
Shigeomi Shimizu ◽  
Makoto Ohtsu ◽  
Masato Takimoto ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Potential Loss ◽  
Mitochondrial Membrane Potential Loss
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