scholarly journals In vitro multiple shoot regeneration from corm bud explant in ghet kachu, typhonium trilobatum schott - a medicinal aroid

2012 ◽  
Vol 47 (2) ◽  
pp. 211-216
Author(s):  
KK Paul ◽  
MA Bari
Keyword(s):  
Shoot Regeneration ◽  
White Light ◽  
Callus Formation ◽  
In Vitro Regeneration ◽  
Multiple Shoot ◽  
Ms Medium ◽  
Multiple Shoot Regeneration ◽  
Regenerated Plantlets ◽  
Light Green

An efficient in vitro regeneration protocol was developed in medicinal aroid, Ghetkachu (Typhonium trilobatum Schott) using field grown corm bud explant. Highest percentage (75 %) of direct multiple shoot regeneration obtained in MS media supplemented with 5.0 mgL-1BAP + 1.5mg L-1NAA. Callus formation occur (80 %) in MS media containing 0.5mgL-1BAP + 2.0mgL-1NAA. The appearance of calli was white, creamy white light green in colour and the texture of calli were soft, friable and semi hard and compact. Shoot regeneration (85 %) obtained from calli in MS medium having 5.0mgL-1BAP +1.0mgL-1NAA. The regenerated plantlets were successfully acclimatized with loamy fertile soil and survived cent percentage in natural condition.   DOI: http://dx.doi.org/10.3329/bjsir.v47i2.11454   Bangladesh J. Sci. Ind. Res. 47(2), 211-216, 2012  

scholarly journals In- vitro regeneration of an important medicinal plant Centella asiatica L. Urban

2012 ◽  
Vol 47 (3) ◽  
pp. 269-272 ◽  
Author(s):  
Dr Jaheduzzaman ◽  
MA Habib ◽  
S Akter ◽  
NA Banu ◽  
RB Rahman ◽  
...  
Keyword(s):  
Shoot Regeneration ◽  
In Vitro Regeneration ◽  
Centella Asiatica ◽  
Multiple Shoot ◽  
Garden Soil ◽  
Nodal Segment ◽  
Root Induction ◽  
Ms Medium ◽  
Multiple Shoot Regeneration

An in vitro multiple shoot regeneration protocol has been developed for Centella asiatica L. Urban from the nodal and shoot tips explants using MS medium with different concentrations and combinations of growth regulators. Multiple shoot regeneration was observed from both the explants on MS containing either BAP alone or in combination with IAA, NAA and Kn. Of the two explants, nodal segment showed comparatively better response towards multiple shoot regeneration. Maximum multiple shoots were found on MS supplemented with 1.0 mg/L BAP and 0.4 mg/L NAA. For root induction, well-developed shoots were excised and cultured on both MS and half strength of MS with various concentrations of IBA, IAA and NAA. Profuse healthy rooting was obtained on MS medium containing 0.2 mg/L IBA. The well rooted plantlets were successfully transplanted to the garden soil and their survival rate under natural condition was 90-95 %. DOI: http://dx.doi.org/10.3329/bjsir.v47i3.13058 Bangladesh J. Sci. Ind. Res. 47(3), 269-272, 2012

scholarly journals In vitro regeneration of Indigofera viscosa lam

2016 ◽  
Vol 22 ◽  
pp. 53-58 ◽  
Author(s):  
E Rajabudeen ◽  
A Saravana Ganthi ◽  
S Sivasubramanian ◽  
M Padma Sorna Subramanian
Keyword(s):  
Callus Formation ◽  
In Vitro Regeneration ◽  
Shoot Multiplication ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Genetic Enhancement ◽  
High Survival ◽  
Ms Medium ◽  
Multiple Shoot Regeneration

Context: In vitro propagation or tissue culture of plants offers a rapid means of producing large quality of clonal planting stock and propagation that are difficult to establish conventionally. Biotechnological tools are important for multiplication and genetic enhancement of the plants by adopting techniques such as in vitro regeneration and genetic transformations.Objective: Effect of different plant growth regulators (PGRs) and their concentration on multiple shoot regeneration and callus formation was studied in Indigofera viscosa.Materials and Methods: In vitro plant regeneration was achieved in nodal and shoot tip explants. The explants were cultured on MS medium supplemented with BAP and NAA.Results: The nodal explants exhibited a greater number of healthy multiple shoots. The maximum callus induction was observed on MS medium supplemented with 1.5 mg L-1 2,4-D. NAA and BAP combination proved to be the most effective treatment for promoting shoot multiplication. IBA was found to be the best rooting hormone than IAA or NAA. The plantlets showed high survival rate in the soil.Conclusion: The present investigation clearly established and demonstrated the method of obtaining the new plantlets in Indigofera viscosa supported by different hormone concentrationsJ. bio-sci. 22: 53-58, 2014

scholarly journals An Efficient Protocol for High-frequency Direct Multiple Shoot Regeneration from Internodes of Peppermint (Mentha x piperita)

2010 ◽  
Vol 5 (12) ◽  
pp. 1934578X1000501
Author(s):  
Sanjog T. Thul ◽  
Arun K. Kukreja
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoots ◽  
Shoot Elongation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Ms Medium ◽  
Mentha X Piperita ◽  
Half Strength ◽  
Multiple Shoot Regeneration

A simple, repeatable and efficient protocol for direct multiple shoot regeneration from internodal explants has been defined in peppermint ( Mentha x piperita var. Indus). In vitro regenerated shoots of peppermint were excised into 4 to 8 mm long internodes and cultured on Murashige and Skoog's medium supplemented with different cytokinins. In the hormonal assay, 3.0 mg L-l zeatin or 6-isopentenyl adenine independently supplemented to half strength MS medium exhibited multiple shoot regeneration, while thiaduzorn (0.1-3.0 mg L−1) showed no morphogenetic effect. A maximum of 85% in vitro cultured explants showed multiple shoot formation with an average of 7 shoots per explant on MS medium supplemented with zeatin. Multiple shoots were initiated within three weeks of cultivation. Internodes with regenerated multiple shoots were transferred to half - strength MS medium without supplementing with any plant growth hormone for shoot elongation and rhizogenesis. Rooted plants acclimatized and grew to maturity under glasshouse conditions. The plantlets developed were phenotypically identical to the parent plant and exhibited 96 % survival.

scholarly journals In vitro regeneration of two high-yielding eggplant (Solanum melongena L.) varieties of Bangladesh

1970 ◽  
pp. 08-12
Author(s):  
Sabina Yesmin, Mst Muslima Khatun, Tanzena Tanny ◽  
Anica Tasnim Protity ◽  
Md Salimullah ◽  
Iftekhar Alam
Keyword(s):  
In Vitro Regeneration ◽  
Solanum Melongena ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Healthy Plant ◽  
Ms Medium ◽  
Best Response ◽  
Half Strength ◽  
Multiple Shoot Regeneration

An in vitro regeneration protocol was developed for two high-yielding eggplant varieties (Solanum melongena L.) namely BARI begun-4 and BARI begun-6. Multiple shoots were regenerated from cotyledonary explants through organogenesis with growth regulators of different combinations and concentrations.  The best response towards multiple shoot regeneration was achieved from cotyledon explants on MS media complemented with 1 mg/l BAP + 0.2 mg/l IAA in both the two varieties of eggplant. Elongation of shoots was achieved on hormone free MS medium. Regenerated shoots of both the varieties produced   active in vitro root system on half strength of MS medium supplemented with 0.2 mg/l IBA.  The in vitro grown plantlets were acclimatized in soil, grew up to maturity, flowered, fruited and produced seeds as normal healthy plant like the control.

scholarly journals In Vitro Mutation of Capsicum annuum L.var. Kulai by Gamma Radiation

2019 ◽  
Vol 8 (4) ◽  
pp. 6934-6938
Keyword(s):  
Seed Germination ◽  
Gamma Radiation ◽  
Capsicum Annuum ◽  
Shoot Regeneration ◽  
Gamma Ray ◽  
Callus Formation ◽  
In Vitro Regeneration ◽  
Germination Rate ◽  
Ms Medium

The present work was carried out to investigate the effects of gamma radiation on regeneration of Capsicum annuum L. var Kulai via in vitro. Seeds of C. annuum were irradiated with various doses of gamma ray (0, 20, 40, 60, 80, 100, 200, 300, 400, 500, and 600 Gy) emitted from the Caesium-137 source at the rate of 4.31 Gy per minute. Irradiated seeds grown on MS medium without hormone for hypocotyl and cotyledon preparation as explant for in vitro regeneration. Seed germination rate revealed significant variation between treatments, and seeds started to germinate between 6 to 17 days. Irradiated seeds between 0-60 Gy were observed to germinate in less than 10 days. All explants including hypocotyl and cotyledon were cultured on MS medium with different concentrations of BAP in combination with AgNO3 to observe the response of these explants to different hormone concentrations. From the observation, calluses were induced in 90% of hypocotyl and cotyledon explants in all treatments. The characteristics of calluses were varied with greenish friable, greenish compact, yellowish watery, yellowish friable and yellowish compact. In other treatments, calluses were found in purple, bright yellow and yellowish orange. On the other hand, shoot regeneration was observed in treatment between 40-100 Gy. In conclusion, gamma radiation gave impact on seed germination, seedling growth performance, in vitro callus formation and shoot regeneration of Capsicum annuum var. Kulai

scholarly journals In vitro Plant Regeneration of Four Local Varieties of Chickpea (Cicer arietinum L.) Grown in Bangladesh

1970 ◽  
Vol 46 (3) ◽  
pp. 379-384 ◽  
Author(s):  
TA Banu ◽  
RH Sarker ◽  
MI Hoque
Keyword(s):  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Mature Embryo ◽  
Multiple Shoot ◽  
Direct Organogenesis ◽  
Ms Medium ◽  
Regeneration System ◽  
Best Response ◽  
Multiple Shoot Regeneration

In vitro regeneration system was developed through direct organogenesis from decapitated mature embryo explants of locally grown four chickpea varieties, namely, Barichhola-4, Hyprochhola, Binachhola-3 and Binachhola-4. Best response towards multiple shoot regeneration was obtained on MS medium supplemented with 0.5 mg/l BAP, 0.5 mg/l Kn, 0.2 mg/l NAA along with double concentrations of CaCl2 and NH4NO3. However good shoot health and expanded leaf was found on MS medium containing 1.0 mg/l kn. Apart from this, few experiments were conducted with decapitated embryo attached cotyledon. Using this explants highest number of multiple shoots were obtained on MSB medium containing 4× micronutrients of MS medium with 3.0 mg/l BAP and 0.04 mg/l NAA in all four varieties. Shoots regenerated on 1.0 mg/l kn supplemented medium showed good response towards rooting on MS medium supplemented with 0.2 mg/l IBA in all four varieties. It was observed that micrografting is an alternative technique to in vitro rooting in chickpea. Key words: In vitro regeneration; Decapitated embryo; Chickpea. DOI: http://dx.doi.org/10.3329/bjsir.v46i3.9047 BJSIR 2011; 46(3): 379-384

scholarly journals Ascorbic Acid Controls Lethal Browning and Pluronic F-68 Promotes High-frequency Multiple Shoot Regeneration from Cotyldonary Node Explant of Okra (Abelmoschus esculentus L.)

HortScience ◽  
2018 ◽  
Vol 53 (2) ◽  
pp. 183-190 ◽  
Author(s):  
Muhammad Irshad ◽  
Hafiz Muhammad Rizwan ◽  
Biswojit Debnath ◽  
Muhammad Anwar ◽  
Min Li ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Plant Growth ◽  
In Vitro Regeneration ◽  
Basal Medium ◽  
Cotyledonary Node ◽  
Multiple Shoot ◽  
Abelmoschus Esculentus ◽  
Ms Medium ◽  
Multiple Shoot Regeneration

The regeneration frequency of okra (Abelmoschus esculentus) is greatly influenced by its genetic makeup and recalcitrant nature. Phenolic secretion, in particular, is a major problem in okra tissue culture. This study describes a reproducible, rapid, and more efficient in vitro regeneration method using cotyledonary node explants of okra. Explants were incubated on Murashige and Skoog (MS) medium containing different concentrations and combinations of various plant growth regulators (PGRs) [benzyladenine (BA), thidiazuron (TDZ), and α-naphthylacetic acid (NAA)], and regeneration enhancers [silver nitrate (AgNO3) and Pluronic F-68]. Cut ends of cotyledonary node segments rapidly turned brown and cultures failed to establish. Antibrowning additives, such as activated charcoal (AC), ascorbic acid (AA), and AgNO3 at various concentrations in PGR-free MS basal medium were tested for their ability to control phenolic secretion from explants. Among these additives, 15 mg·L−1 AA was found to be optimal for controlling phenolic secretion, resulting in healthy explants and culture establishment. The highest number of shoots (a mean of 9.3 ± 0.9 shoots per cotyledonary node explant) was obtained on MS media containing 0.5 mg·L−1 NAA + 1 mg·L−1 TDZ + 0.1% Pluronic F-68. Individual shoots were elongated on MS medium + 1 mg·L−1 BA + 0.1 mg·L−1 gibberellic acid (GA3) (shoot length 5.3 ± 0.2 cm) and rooted on ½ MS medium + 1 mg·L−1 indole-3-butyric acid (IBA) and 200 mg·L−1 AC (5.3 ± 0.2 roots per shoot). Rooted plantlets were acclimatized in plastic pots inside a plant growth chamber at 25 ± 2 °C and 70% relative humidity, with an 80% survival rate. This optimized protocol can be used for producing transgenic plants of commercial okra cultivars through genetic transformation.

scholarly journals In vitro Plant Regeneration in Mungbean (Vigna radiate (L.) Wilczek)

1970 ◽  
Vol 17 (2) ◽  
pp. 209-216 ◽  
Author(s):  
M.I. Hoque ◽  
Most. Mosfeqa Zahan ◽  
R.H. Sarker
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoot ◽  
In Vitro Plant Regeneration ◽  
Vigna Radiate ◽  
Best Response ◽  
Efficient Regeneration ◽  
Half Strength ◽  
Multiple Shoot Regeneration ◽  
Regenerated Plantlets

An efficient regeneration protocol without the intervention of callus using cotyledon explants of three mungbean varieties namely, BARI mug-3, BARI mug-5 and BINA mug-5 was developed. Best response toward multiple shoot regeneration in BARI mug-5 and BINA mug-5 was observed on MS supplemented with 0.5 and 0.5 mg/l Kn. However, for BARI mug-3, MS supplemented with 0.5 mg/l BAP and 0.1 mg/l Kn was found to be most effective for multiple shoot regeneration. Half-strength of MS supplemented with 0.5 mg/l IBA was found to induce healthy roots from the excised shoots in all the three mungbean varieties. In vitro regenerated plantlets with well developed roots were successfully established in soil.  Key words: Regeneration protocol, mungbean, Vigna radiata D.O.I. 10.3329/ptcb.v17i2.3241 Plant Tissue Cult. & Biotech. 17(2): 209-216, 2007 (December)

scholarly journals An Improved Protocol for Multiple Shoot Regeneration from Seedlings and Mature Explants of Citrus macroptera Mont.

2009 ◽  
Vol 18 (1) ◽  
pp. 17-24
Author(s):  
Md. Nesawar Miah ◽  
Shahina Islam ◽  
Syed Hadiuzzaman
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Nodal Explants ◽  
Cow Dung ◽  
Half Strength ◽  
Multiple Shoot Regeneration ◽  
Shoot Tip Explants ◽  
In Vitro Shoot Regeneration

Efforts have been made to establish a protocol for direct multiple shoot regeneration from both in vitro grown seedlings and mature plants of Citrus macroptera. Both nodal and shoot tip explants taken from in vitro grown seedlings were cultured in MS supplemented with different concentrations of BAP and Kn either singly or in combinations. Both these explants are capable to regenerate and produce in vitro multiple shoots. Maximum number of shoots were obtained from nodal explants in MS supplemented with 1.0 mg/l BAP. BAP alone was found superior to Kn. On the other hand, only nodal explants from mature plants were used and 1.0 mg/1 BAP was also found best suitable for shoot induction and multiplication. Ex vitro rooting in pot soil (mixed with biogas slurry derived from cow-dung) was most successful compared to in vitro rooting in half strength of MS supplemented with different concentrations of NAA and IBA. Key words: In vitro, Shoot regeneration, Citrus macroptera D.O.I. 10.3329/ptcb.v18i1.3246 Plant Tissue Cult. & Biotech. 18(1): 17-24, 2008 (June)

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