In vitro mass propagation of Piper betle L

2019 ◽  
Vol 48 (3) ◽  
pp. 559-566
Author(s):  
Salim Khan ◽  
Barna Goswami ◽  
Shahina Akter ◽  
Mousona Islam ◽  
Afsana Huq Noon ◽  
...  
Keyword(s):  
Nodal Explants ◽  
Direct Regeneration ◽  
Leaf Segment ◽  
Piper Betle ◽  
Nodal Segment ◽  
Root Induction ◽  
Regeneration System ◽  
Petiole Explants ◽  
Best Response

An efficient in vitro regeneration system was developed for Piper betle L. through direct and indirect organogenesis from nodal segment, leaf segment and petiole explants. Highest direct regeneration was recorded when nodal explants were cultured on MS with 1.0 mg/l BAP and 1.0 mg/l Kn where 80% explants produced multiple shoots and the average number of shoots per explants were 3.20. Remarkable results on callus induction and shoot initiation were observed when the explants cultured on MS + 2.0 mg/l BAP + 0.5 mg/l Kn + 1.0 mg/l IAA. It was observed that nodal explants were showed best response on shoot/explants 13.2 ± 4.5 after 8 weeks of callus culture on MS medium with 0.5 mg/l BAP. The best response towards root induction was observed on half strength of MS with 0.25 mg/l IBA. The well rooted plants were successfully acclimatized and transferred to soil.

scholarly journals In vitro Regeneration in Three Varieties of White jute (Corchorus capsularis L.)

1970 ◽  
Vol 17 (1) ◽  
pp. 11-18 ◽  
Author(s):  
RH Sarker ◽  
GM Al-Amin ◽  
MI Hoque
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
The Other ◽  
Nodal Explants ◽  
Root Induction ◽  
Corchorus Capsularis ◽  
Best Response ◽  
Multiple Shoot Regeneration

Healthy multiple shoot regeneration was observed from petiole-attached cotyledon (PC) explants of CVL-1 on MS containing 0.2 mg/l BAP and 1.0 mg/l IAA. On the other hand, the best response for multiple shoot regeneration in CVE-3 was obtained when the same explants were cultured on MS with 2.5 mg/l BAP and 0.5 mg/l NAA. However, the same explant of D-154 was found to show less responsive compared to other two varieties to produce multiple shoots. Cotyledonary nodal explants of all the three varieties were found to produce maximum number of multiple shoots on MS supplemented with 0.2 mg/l BAP and 1.0 mg/l IAA. Best root induction was observed at the base of the in vitro regenerated shoots on half the strength of MS supplemented with 0.3 mg/l IBA. The in vitro grown plantlets were successfully transplanted into soil. They grew up to maturity, flowered and fruited like the control plants.Key words: Regeneration, White jute, Corchorus capsularisDOI = 10.3329/ptcb.v17i1.1116Plant Tissue Cult. & Biotech. 17(1): 11-18, 2007 (June)

scholarly journals High Frequency In vitro Regeneration of Gynura procumbens (Lour.) Merr.

2017 ◽  
Vol 27 (2) ◽  
pp. 207-216
Author(s):  
Tanjina Akhtar Banu ◽  
Barna Goswami ◽  
Shahina Akter ◽  
Mousona Islam ◽  
Tammana Tanjin ◽  
...  
Keyword(s):  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Root Induction ◽  
Petiole Explants ◽  
Number Of Shoots

An efficient rapid in vitro regeneration protocol was described from nodal segment, leaf and petiole explants. MS medium supplemented with 1.0 mg/l BAP and 0.5 mg/l IAA was found best for the multiple shoot formation from nodal segments. In this combination 99% explants produced multiple shoots and the average number of shoots per explants was 20.1 ± 1.96. For petiole and leaf explants best response was observed on MS supplemented with 2.0 mg/l BAP, 1 mg/l IAA and 0.5 mg/l Kn. Petiole explants produced highest mean number of shoots/explant (22.9 ± 1.728) among the three explants when the explants were cultured on MS with 2.0 mg/l BAP, 1 mg/l IAA and 0.5 mg/l Kn. The highest frequency of root induction (100%) and mean number of roots/plantlets (11.75) were obtained on MS. The rooted plantlets were transferred for hardening following acclimatization and finally were successfully established in the field.Plant Tissue Cult. & Biotech. 27(2): 207-216, 2017 (December)

scholarly journals Micropropagation of Environmental Stress Tolerant Local Potato (Solanum tuberosum L.) Varieties of Bangladesh

2014 ◽  
Vol 24 (1) ◽  
pp. 101-109 ◽  
Author(s):  
Fazlima Parveen ◽  
Mahmuda Khatun ◽  
Aparna Islam
Keyword(s):  
Shoot Apex ◽  
Large Scale ◽  
Solanum Tuberosum L ◽  
In Vitro Regeneration ◽  
Nodal Explants ◽  
Direct Regeneration ◽  
Nodal Segment ◽  
Scale Production ◽  
Potato Varieties

In vitro response of four explants namely, leaf, shoot apex, nodal and internodal, in three stress tolerant Bangladeshi potato varieties, viz. Zaubilati, Shadaguti and Challisha were tested. Of all the varieties, Shadaguti responded best for all the explants. Among the explants nodal segment responded best, followed by shoot apex. For all these explants and varieties, shoot regeneration response was tested in response to two cytokines, BAP and Kn. When compared between BAP and Kn supplementation, Kn treatment performed better than that of BAP for nodal and shoot apex, while opposite was observed for remaining explants. Interestingly, hormone free basal PROP medium was found to be best for nodal explants of both Zaubilati and Challisha varieties. While nodal explants of Shadaguti showed the best result in 0.5 mg/l Kn. Shoot apex  performed best in 0.5 mg/l Kn  for all the varieties. As an explant both internode and leaf did not perform well. Direct regeneration from these explants was found best in PROP + 0.2 mg/l GA3 + 0.5 mg/l IAA;  1.0 mg/l BAP, 1.5 mg/l BAP and 2.0 mg/l BAP for Zaubilai, Challisha and Shadaguti, respectively. For rooting, of the in vitro grown shoots half strength of PROP medium supplemented with 0.5 mg/l IBA was found to be best. Cent percent survival of transplanted plantlets was recorded. The successful protocol for in vitro regeneration was developed which can be used for large scale production of these abiotic stress tolerant potato varieties. Plant Tissue Cult. & Biotech. 24(1): 101-109, 2014 (June) D. O. I. http://dx.doi.org/10.3329/ptcb.v24i1.19218

scholarly journals In vitro regeneration in pointed gourd

2008 ◽  
Vol 32 (3) ◽  
pp. 461-471 ◽  
Author(s):  
MA Malek ◽  
MA Bari Miah ◽  
M AL-Amin ◽  
D Khanam ◽  
M Khatun
Keyword(s):  
Shoot Regeneration ◽  
Nodal Segment ◽  
Root Induction ◽  
Ms Medium ◽  
Shoot Induction ◽  
Best Response ◽  
Pointed Gourd ◽  
Regenerated Plantlets ◽  
Better Than

An efficient protocol was developed for plant regeneration, multiplication and rooting under in vitro condition in pointed gourd. Highest percent of shoot regeneration was 93.86 when nodal explants were cultured on MS+2.0 mg/1 BAP. The maximum number of shoots (4.00) per explant was observed in MS + 2.0 mg/1 BAP + 0.3 mg/1 NAA from nodal segment. Among the two explants, nodal segment was found better for shoot regeneration. Female genotypes responded better than the male genotypes for shoot induction and proliferation. Lower nodal segment performed the best shoot regeneration. The best response towards root induction was achieved on half MS medium supplemented with 0.5 mg/1 NAA. The regenerated plantlets were successfully established in prepared earthen soil pot.DOI: http://dx.doi.org/10.3329/bjar.v32i3.548Bangladesh J. Agril. Res. 32(3) : 461-471, September 2007

scholarly journals IN VITRO PROPAGATION OF SHOOTS AND CALLUS INDUCTION OF GYMNEMA SYLVESTRE R. BR. “AN IMPORTANT ANTI-DIABETIC PLANT”

2018 ◽  
Vol 10 (3) ◽  
pp. 60 ◽  
Author(s):  
Mohsina Syedy ◽  
Krishnendra Singh Nama
Keyword(s):  
Callus Induction ◽  
Research Work ◽  
Multiple Shoots ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Root Induction ◽  
Ms Medium ◽  
Petiole Explants ◽  
Leaf Petiole

Objective: The objective of this research was to establish and develop a protocol for the mass multiplication and callus induction of an anti-Diabetic plant-G. sylvestre R. Br.Methods: Sterilized explants (Nodal segment and leaf) were used for the initiation of culture. They were cultured on MS medium supplemented with a variety of PGRs (BAP, Kn, IBA, 2,4-D) individually or in combinations.Results: The induction of multiple shoots from nodal segments were highest in MS medium supplemented with 2.0 mg/l Kn and in BAP Maximum shoots were obtained on MS medium fortified with 1 mg/l BAP. For rooting different concentration of IBA were used and highest rooting was recorded on MS medium supplemented with 2.0 mg/l IBA. The rooted Plantlets were hardened initially in culture room conditions and then transferred to mist house. Leaf petiole explants were used for the purpose of callus induction. Best growth was observed in MS medium supplemented with 2,4-D. 1.0 mg/l 2,4-D+0.5 mg/l BAP, 1.0 mg/l 2,4-D+1.5 mg/l Kn.Conclusion: The results obtained in this research work clearly indicated that Kn is a better choice than BAP for the culture initiation. 2 mg/l IBA was proved best for root induction. For callus induction, 1 mg/l 2,4-D gave good results and when callus was sub-cultured on 2,4-D with BAP or Kn then 1.0 mg/l 2, 4-D+1.5 mg/l Kn proved best for mass propagation of callus.

scholarly journals In vitro micropropagation of pointed gourd (Trichosanthes dioica Roxb.) from shoot tip and nodal segment

2012 ◽  
Vol 47 (2) ◽  
pp. 217-222 ◽  
Author(s):  
Amdadul Huq ◽  
Shahina Akter ◽  
Shahina Islam ◽  
Salim Khan
Keyword(s):  
Shoot Regeneration ◽  
Garden Soil ◽  
Nodal Segment ◽  
Root Induction ◽  
Ms Medium ◽  
Best Response ◽  
In Vitro Micropropagation ◽  
Trichosanthes Dioica ◽  
Pointed Gourd

An efficient protocol was developed for plant regeneration, multiplication and rooting under in vitro condition in pointed gourd. Highest percent of shoot regeneration was 91.66%, when nodal explants were cultured on MS+1.0 mg/1 BAP. The maximum number of shoots (4.8) per explant was observed in MS + 1.0 mg/1 BAP + 0.2 mg/1 NAA from nodal segment. Among the two explants, nodal segment was found better for shoot regeneration and multiplication. The best response towards root induction was achieved on half MS medium supplemented with 0.4 mg/1 NAA. The regenerated healthy rooted plantlets were transferred to small plastic pot containing garden soil and compost in a ratio of 2:1. Immediately after transplantation the plantlets were covered with polythene bag to prevent desiccation. After acclimatization the plantlets were successfully transferred to the garden soil and the percentage of survivability in such condition was 90%.   DOI: http://dx.doi.org/10.3329/bjsir.v47i2.11457   Bangladesh J. Sci. Ind. Res. 47(2), 217-222, 2012  

scholarly journals In vitro regeneration of three varieties of Brassica campestris L. grown in Bangladesh

2020 ◽  
Vol 55 (3) ◽  
pp. 181-188
Author(s):  
B Goswami ◽  
MI Hoque ◽  
S Khan ◽  
RH Sarker
Keyword(s):  
Shoot Regeneration ◽  
Brassica Campestris ◽  
In Vitro Regeneration ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Root Induction ◽  
Regeneration System ◽  
Cotyledonary Leaf ◽  
Best Response

A reproducible in vitro regeneration system was developed for three varieties of Brassica campestris L. namely Agrani, BINA Sarisha-10 and BINA Sarisha-6 using hypocotyl and cotyledonary leaf with petiole as explants. MS medium supplemented with 2.0 mg/l BAP and 0.2 mg/l IAA was found to be the best for the multiple shoot formation for all the three varieties. Among three varieties, BINA Sarisha-6 showed best response in terms of shoot regeneration as well as number of shoots per explant (9.0) using hypocotyls as explants. In case of Agrani and BINA Sarisha-10 highest number of shoot per explants were found 8.2 and 7.0, respectively. Interestingly in vitro regenerated shoots of BINA Sarisha-6 and BINA Sarisha-10 were produced in vitro flowers on shoot regeneration media. Best root induction in BINA Sarisha-6, Agrani and BINA Sarisha-10 was achieved on MS media supplemented with 0.5 mg/l IBA. After proper hardening, the in vitro regenerated plantlets were successfully transplanted into soil. Bangladesh J. Sci. Ind. Res.55(3), 181-188, 2020

scholarly journals In vitro regeneration protocol of Rauvolfia serpentina L.

2018 ◽  
Vol 53 (2) ◽  
pp. 133-138 ◽  
Author(s):  
S Khan ◽  
TA Banu ◽  
S Akter ◽  
B Goswami ◽  
M Islam ◽  
...  
Keyword(s):  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Multiple Shoot ◽  
Nodal Segments ◽  
Root Induction ◽  
Ms Medium ◽  
Regeneration System ◽  
Rauvolfia Serpentina ◽  
Number Of Shoots

An efficient in vitro regeneration system was developed for Rauvolfia serpentina L. through direct and indirect organogenesis from nodal and leaf explants. Among the different growth regulators, MS medium supplemented with 2.0 mg/l BAP, 0.5mg/l IAA and 0.02mg/l NAA found best for the multiple shoot formation from nodal segments. In this combination 98% explants produced multiple shoots and the average number of shoots per explants is 13∙4. The frequency of callus induction and multiple shoot induction from leaves was highest 88% in MS medium supplemented with 2.0 mg/l BAP, where mean number of shoots/explants was 12.5. The highest frequency of root induction (80%) and mean number of roots/plantlets (10) were obtained on half strength of MS medium containing 0.2 mg/l IBA. The rooted plantlets were transferred for hardening following acclimatization and finally were successfully established in the field.Bangladesh J. Sci. Ind. Res.53(2), 133-138, 2018

scholarly journals In vitro study of Tinospora cordifolia (Willd.) Miers (Menispermaceae)

2010 ◽  
Vol 6 ◽  
pp. 103-105 ◽  
Author(s):  
Aditi Singh ◽  
Saroj K Sah ◽  
Aunji Pradhan ◽  
Sabari Rajbahak ◽  
Niran Maharajan
Keyword(s):  
Medicinal Plant ◽  
Callus Induction ◽  
Shoot Growth ◽  
Tinospora Cordifolia ◽  
Nodal Segments ◽  
Naphthaleneacetic Acid ◽  
Nodal Segment ◽  
Root Induction ◽  
Ms Medium

In vitro study was carried out in an important medicinal plant Tinospora cordifolia (Willd.) Miers belonging to the family: Menispermaceae. Vegetative parts such as stem, leaf and nodal explants were excised from an elite in vivo grown mature plant and thereafter cultured on Murashige-Skoog (MS) medium supplemented with different hormonal concentrations for callus induction and organogenesis. Callus formation occurred from nodal segments, leaf and inter-node explants when planted on different combinations of hormones. Tinospora cordifolia showed response for in vitro shoot growth from the nodal segment. The best shoot growth was observed on MS medium supplemented with kinetin (1.5 mg/l). Similarly, the best result for root induction was obtained on MS medium supplemented with 6-benzylaminopurine (1.0 mg/l) and naphthaleneacetic acid (2.5 mg/l). Key-words: callus induction; explants; medicinal plant; MS medium; tissue culture.DOI: 10.3126/botor.v6i0.2918 Botanica Orientalis - Journal of Plant Science (2009) 6: 103-105

scholarly journals In vitro propagation of Citrullus lanatus Thumb. from nodal explants culture

1970 ◽  
Vol 8 (2) ◽  
pp. 203-206 ◽  
Author(s):  
MM Khatun ◽  
MS Hossain ◽  
MA Haque ◽  
M Khalekuzzaman
Keyword(s):  
Citrullus Lanatus ◽  
In Vitro Regeneration ◽  
Shoot Proliferation ◽  
Multiple Shoot ◽  
Nodal Explants ◽  
Root Induction ◽  
Ms Medium ◽  
Nodal Explant ◽  
Grown Plant

A standard protocol was established for rapid in vitro propagation of watermelon (Citrullus lanatus Thumb.) from nodal explants of field grown plant. Multiple shoot proliferation was achieved from nodal explants on MS medium supplemented with 1.0 mg/l BAP + 0.2 mg/l NAA within 30 days of inoculation. The elongation of shoots was obtained on the same medium. Highest percentage of root induction was achieved on MS medium supplement with 1.0 mg/l IBA within 25 days of culture. Well rooted plantlets were transferred to small pots and after proper acclimatization the plantlets were transplanted in the field condition, where 80% plantlets were survived and grew successfully. Keywords: In vitro regeneration; Nodal explant; Citrullus lanatus DOI: 10.3329/jbau.v8i2.7926 J. Bangladesh Agril. Univ. 8(2): 203-206, 2010  

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