scholarly journals Evaluation of total phenolic content, free radical scavenging activity and phytochemical screening of different extracts of Averrhoa bilimbi (fruits)

2013 ◽  
Vol 2 (4) ◽  
pp. 92-96 ◽  
Author(s):  
Md. Hasanuzzaman ◽  
Md. Ramjan Ali ◽  
Marjan Hossain ◽  
Sourov Kuri ◽  
Mohammad Safiqul Islam
Keyword(s):  
Free Radical ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Phytochemical Screening

The present study was designed to investigate the phytochemical screening, the free radical scavenging activity and to determine the total phenolic content of methanolic extract and different solvent soluble fractions of Averrhoa bilimbi Linn. (Oxalidiaceae) fruits. The free radical scavenging activity was evaluated by analyzing the bleaching rate of 1,1-diphenyl-2-picrylhydrazyl (DPPH), and total phenolic content was determined by using Folin-Ciocalteau reagent, which results were expressed in gallic acid equivalent (mg of GAE/g of sample). The phytochemical screening revealed the potent source of different phytochemical constituents on different extractives including, phenol, flavonoid, tannin that are responsible for antioxidant action. In the determination of total phenolic content, different extractives showed a significant content of phenolic compounds ranging from 50.23-68.67 mg of GAE/g of extractive. The plant sample displayed significant DPPH free radical scavenging activity with highest IC50 value in crude methanolic extract (30.365 ?g/ml) followed by chloroform, carbon tetrachloride, pet-ether and aqueous soluble fractions having value of 32.852 ?g/ml, 36.708 ?g/ml, 50.35 ?g/ml, and 79.918 ?g/ml, respectively as opposed to that of the scavenging effects of BHT of 19.656 ?g/ml.DOI: http://dx.doi.org/10.3329/icpj.v2i4.14058 International Current Pharmaceutical Journal, March 2013, 2(4): 92-96

scholarly journals Evaluation of Phenolic Content and Free Radical Scavenging Activity of Indonesia Wild Honey Collected from Seven Different Regions

2019 ◽  
Vol 8 (6) ◽  
pp. 94
Author(s):  
Y. Riswahyuli ◽  
Abdul Rohman ◽  
Francis M. C. S. Setyabudi ◽  
Sri Raharjo
Keyword(s):  
Free Radical ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Flavonoid Content

Several wild honey samples collected from seven different regions in Indonesia were investigated to determine their total phenolic content, flavonoid content, and free radical scavenging activity by analyzing the 1,1-diphenyl-2-picrylhydrazyl (DDPH) radical and phenolic profile. Rutin, (+)-catechin, ferulic acid, and galangin were found to be the major phenolic compounds of Indonesia wild honey. The total phenolic content significantly correlated with the total flavonoid content (p=0.000) and the percentage of DPPH radical scavenging activity (p=0.000). Results indicated that there are different polyphenol profiles among the different regions.

scholarly journals Bioactive Compound (Phenolic, Anthocianin, and Antioxidant) in Black Rice (Oryza sativa var. Pare Ambo) South Sulawesi

2019 ◽  
pp. 143-150
Author(s):  
N Kiay ◽  
A B Tawali ◽  
M M Tahir ◽  
M Bilang
Keyword(s):  
Free Radical ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Black Rice

Cereals are widely found in Indonesia and used as foodstuffs. Nutrient content of cereals vary from quantity and quality. Rice, one kind of cereals is commonly consumed by Indonesian community. There are some various types of rice such as brown rice, black rice and red rice. One type of rice, namely black rice is widely known as antioxidant source, for example black rice. This research be focused on the analysis of bioactive compound in black rice (Oryza sativa var Pare Ambo). This research using laboratory experiment methods to analyze total phenolic content, anthocianin, and antioksidan (free radical scavenging activity DPPH) black rice using spectrophotometer UV-vis. This research using complete random design (rancangan acak lengkap, RAL), thus obtained data were analyzed using statistic. Significant difference between groups were analyzed using ANOVA with p<0,05. The results showed that black rice extract possess to total phenolic content 3,84 mg/kg gallic acid, free radical scavenging activity 81,20%, total antioxidant 28,42 ppm and vitamins B1, B5, B6 mg/100 g sample (p>0,05). Black rice possess to total phenolic content 10,26 mg/kg gallic acid, free radical scavenging activity 62,67%, anthocianin content 122,33%, red rice possess to total phenolic content (TPC) 7,48 mg/kg gallic acid, free radical scavenging activity 54,78%,. Antioxidant activity from black rice caused by it phenolics compound that act as antioxidant. Spectra XRD Analysis for Determination of Vitamin B (Percentage of Vitamins) (B3, B5, B6) niacin, pantothenic acid and Riboflavin results showed the highest percentage of vitamin B5 (40,45). The results of the research were 88,21% carbohydrates, 8.36% protein, 1.74% fat, 11.17% water, 1.15% ash which amounts to 0.395 mg calcium (Ca), 1.98 mg / ml Magnesium (Mg) and 0.387 mg / ml iron (Fe). In conclusion from this study, black rice has antioxidant activity and nutrient content, these products are expected to be native functional foods.

scholarly journals Total Phenolic Content, Free Radical Scavenging Activity and Reducing Power of Quisqualis indica Linn.

1970 ◽  
Vol 8 (2) ◽  
pp. 173-175 ◽  
Author(s):  
Md Abul Kaisar ◽  
Mohammad Rashedul Islam ◽  
Mohammad Sarifur Rahman ◽  
Md Khalid Hossain ◽  
Mohammad A Rashid
Keyword(s):  
Free Radical ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Total Phenolic ◽  
Scavenging Activity

DOI: 10.3329/dujps.v8i2.6034 Dhaka Univ. J. Pharm. Sci. 8(2): 173-175, 2009 (December)

scholarly journals Phytochemical Screening, Free Radical Scavenging and α-Amylase Inhibitory Activities of Selected Medicinal Plants from Western Nepal

Medicines ◽  
2019 ◽  
Vol 6 (2) ◽  
pp. 70 ◽  
Author(s):  
Kusum Sai ◽  
Rashmi Thapa ◽  
Hari Prasad Devkota ◽  
Khem Raj Joshi
Keyword(s):  
Free Radical ◽  
Medicinal Plants ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Phytochemical Screening ◽  
Inhibitory Activities

Background: More than 700 plants are reported to be used for medicinal purposes in Nepal; however, many of them are not studied for their scientific evidences. The aims of the present study were the estimation of the total phenolic and flavonoid contents, and the evaluation of the free radical scavenging and α-amylase inhibitory activities of five selected medicinal plants from western Nepal: Aeschynanthus parviflorus Wall. (Gesneriaceae), Buddleja asiatica Lour. (Loganiaceae), Carica papaya L. (Caricaceae), Drepanostachyum falcatum (Nees) Keng f. (Gramineae) and Spondias pinnata (L. f.) Kurz (Anacardiaceae). Methods: The total phenolic content (TPC) and total flavonoid content (TFC) were measured using Folin-Ciocalteu’s phenol reagent and aluminium chloride methods, respectively. A 1,1–diphenyl–2–picrylhydrazyl (DPPH) free radical scavenging assay was used to evaluate the free radical scavenging activity and an α-amylase inhibitory assay was carried out to determine the in vitro antidiabetic activity. Results: The phytochemical screening of five hydroalcoholic plant extracts revealed the presence of various secondary metabolites, including alkaloids, flavonoids, reducing sugars, saponins, terpenoids and tannins. The amounts of total phenolics and flavonoids were found to be the highest in B. asiatica leaf extract, which also showed the most potent free radical scavenging activity. Extract of C. papaya fruits showed the highest α-amylase inhibitory activity, whereas the extracts of B. asiatica leaves and S. pinnata leaves exhibited moderate activity. Conclusions: Some of the medicinal plants selected in this study showed high TPC and TFC values and potent bioactivities. These results may provide the scientific evidences of the traditional uses of these plants. However, further detailed studies on bioactive compounds isolation and identification and evaluation of in vivo pharmacological activities should be performed in future.

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