scholarly journals In vitro Regeneration of Blepharispermum subsessile DC: An Endangered Medicinal Plant of Odisha, India using Cotyledon Explants

2016 ◽  
Vol 26 (2) ◽  
pp. 255-266 ◽  
Author(s):  
Pranati Nayak ◽  
Kalidass C
Keyword(s):  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Garden Soil ◽  
Induction Medium ◽  
Root Induction ◽  
Shoot Buds ◽  
Shoot Bud ◽  
Cotyledon Explants ◽  
Bud Induction

Multiple shoots were induced on cotyledon explants of in vitro grown seedlings of Blepharispermum subsessile DC, cultured on MS medium supplemented with various combinations and concentrations of BAP, IBA and GA3. The highest regenerative response was observed on medium containing 2.5 mg/l BAP where shoot buds initiated after 12 days of inoculation and about 32 shoots were produced in 30 days time. Addition of GA3 played a key role in leaf expansion and elongation of shoot buds. Addition of the auxin IBA to the induction medium resulted in more callus proliferation rather than shoot bud induction. The elongated shoots were transferred to root induction medium consisting of half strength MS supplemented with IAA, NAA and IBA. Highest rooting response (90%) was recorded in ½ MS supplemented with 1.0 mg/l IAA. Acclimatized plants were maintained in polybags with garden soil for future reintroduction program to their natural habitat.Plant Tissue Cult. & Biotech. 26(2): 255-266, 2016 (December)

scholarly journals Establishment of in vitro regeneration protocol for Adhatoda vasica Nees

2016 ◽  
Vol 51 (1) ◽  
pp. 75-80 ◽  
Author(s):  
S Khan ◽  
S Akter ◽  
A Habib ◽  
TA Banu ◽  
M Islam ◽  
...  
Keyword(s):  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Nodal Segments ◽  
Garden Soil ◽  
Root Induction ◽  
Ms Medium ◽  
Adhatoda Vasica

An in vitro regeneration protocol of Adhatoda vasica has been developed using excised nodal segments and juvenile leaves for multiple shoots regeneration directly or through callus induction. Explants were cultured on MS medium with different concentrations of IAA, NAA, BAP, GA3 and Kn singly or in combinations. MS medium supplemented with BAP (10.0 mg/l) was found best for multiple shoot formation, in which 93.33% explants produced multiple shoots. After two months, maximum number of multiple shoots were 10.6 ± 1.82, highest length of plantlets was 5.2 ± 2.20 cm. 100% calli formation were observed on MS medium supplemented with IAA (0.05 mg/l) + NAA (0.05 mg/l) + BAP (1.0 mg/l). Callus initiation started after 14 days and gave light green colored callus. Best callus mediated shoot regeneration was found on MS+10.0 mg/l BAP medium. Root induction of in vitro raised shoots was best on ½ MS + IBA (1.0 mg/l). Well rooted plantlets were transferred to plastic pots containing garden soil and compost in a ratio of 2:1 for hardening. The ultimate survival rate under natural condition was about 80%.Bangladesh J. Sci. Ind. Res. 51(1), 75-80, 2016

scholarly journals Induction of shoot buds, multiplication and plantlet formation in seedling explants of bell pepper (Capsicum annuum L.) cv. Bryza in vitro

2014 ◽  
Vol 71 (3) ◽  
pp. 187-193
Author(s):  
Andrzej Gatz
Keyword(s):  
Capsicum Annuum ◽  
Cotyledonary Node ◽  
Explant Culture ◽  
Shoot Tip ◽  
Shoot Buds ◽  
Plantlet Formation ◽  
Shoot Bud ◽  
Cotyledon Explants ◽  
Bud Induction

In vitro shoot bud induction and multiplication as well as plantlets formation from different parts of 21-d old seedlings (shoot tip, cotyledonary node, distal part of cotyledon, acropetal section of hypocotyl) of <em>Capsicum annuum</em> L., cv. Bryza were compared. During 4 weeks of primary explant culture on initiation media, first shoot bud primordia appeared; they reminded leaf primordia and subsequently some of them underwent enlargement, some developed into leaves and leaf-like structures (mainly on cotyledon explants). The highest number of shoot bud primordia was noted on cotyledonary node explants, but they were smaller than those on the remaining types of the explants. The best response of shoot regeneration showed cotyledon explants on which most of shoot buds were formed in each from four treated passages. From shoot buds on elongation media after 4 weeks of culture rooted rosettes of leaves were achieved, and the extension of the culture time to eight weeks without subculture caused that the rosettes developed into plantlets. Throughout four successive passages plantlets were obtained from cotyledon and shoot tip explants.

scholarly journals Glutamine enhances competence for organogenesis in pineapple leaves cultivated in vitro

2005 ◽  
Vol 17 (4) ◽  
pp. 383-389 ◽  
Author(s):  
Regina M. Hamasaki ◽  
Eduardo Purgatto ◽  
Helenice Mercier
Keyword(s):  
Direct Organogenesis ◽  
Induction Medium ◽  
Induction Phase ◽  
Regeneration Rate ◽  
Shoot Buds ◽  
Shoot Bud ◽  
Bud Induction ◽  
Shoot Induction Medium ◽  
Pineapple Leaves

Leaf bases of pineapple cultured on a shoot induction medium (SIM) produced protuberances followed by shoot-buds via direct organogenesis at a frequency of 46 %. When 8 mM glutamine (gln) was a supplement to SIM (SIM8gln), the regeneration rate increased to 70 %, thus suggesting that 8mM gln increased explant competence for organogenesis. Besides this, shoot vigor was strongly enhanced in SIM8gln. Other gln concentrations (16 or 32 mM) evoked a lower frequency of shoot-bud induction and number of regenerated shoots per explant when compared to SIM8gln. In this study, it was defined that explant organogenic commitment to form shoot-buds occurred in the first 7 days of culture on SIM8gln. Thereafter, endogenous indole-3-acetic acid (IAA) and cytokinin (4 types) measurements were carried out during this period, that is, during the induction phase of shoot-bud formation. The IAA content increased greatly until the 5th day in the leaf bases cultured on SIM8gln. No such change in IAA concentration was observed in the explants cultivated on SIM or in the presence of the highest gln concentration (32 mM), this being inhibitory to the organogenic process. The only natural cytokinin detected was isopentenyladenine. An increase of 50 % in the level of this phytohormone occurred in leaf bases cultured on SIM8gln at the 5th day, when compared to SIM or of 170% compared to SIM32gln. These results suggest that 8 mM gln favorably influenced the organogenic process through changes in IAA and iP concentrations in pineapple leaves.

scholarly journals In Vitro Regeneration of Castor (Ricinus Communis L.) Using Cotyledon Explants

HortScience ◽  
2008 ◽  
Vol 43 (1) ◽  
pp. 215-219 ◽  
Author(s):  
Yeh-Jin Ahn ◽  
Grace Qianhong Chen
Keyword(s):  
Ricinus Communis ◽  
In Vitro Regeneration ◽  
Adventitious Shoots ◽  
Shoot Elongation ◽  
Induction Medium ◽  
Root Induction ◽  
Oilseed Crop ◽  
Ms Medium ◽  
Cotyledon Explants

An efficient plant regeneration protocol using cotyledon explants was established for castor (Ricinus communis L.), an important oilseed crop. Mature seed-derived cotyledon explants produced adventitious shoots when placed on Murashige and Skoog (MS) medium containing thidiazuron (TDZ). The rate of shoot regeneration was maximal (≈25 shoots per explant) when explants were cultured on shoot induction medium supplemented with 5 μm TDZ and preincubated in the dark for the first 7 days before transferring to the day/night cycle (16/8 h). Only the proximal ends of cotyledon explants produced adventitious shoots, although green calli were observed in cotyledon veins. After 4 weeks in culture, explants with well-developed shoot buds were transferred to MS medium without plant growth regulators for the shoot elongation and development. At ≈4 months after culture initiation, shoots (2 cm in length) were transferred to root induction medium (MS medium supplemented with 5 μm indole-3-butyric acid) where they developed roots in 4 to 6 weeks. Plantlets were transferred to soil and acclimatized to greenhouse conditions. Histological analysis showed the adventitious induction of the shoots originated from the cortical and epidermal cell layers of the cotyledon explants.

scholarly journals In vitro Regeneration and Agrobacterium-mediated Genetic Transformation of Tomato (Lycopersicon esculentum Mill.)

1970 ◽  
Vol 19 (1) ◽  
pp. 101-111 ◽  
Author(s):  
Rakha Hari Sarker ◽  
Khaleda Islam ◽  
M.I. Hoque
Keyword(s):  
Lycopersicon Esculentum ◽  
Genetic Transformation ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Root Induction ◽  
Cotyledonary Leaf ◽  
Lycopersicon Esculentum Mill

Agrobacterium-mediated genetic transformation system has been developed for two tomato (Lycopersicon esculentum Mill.) varieties, namely Pusa Ruby (PR) and BARI Tomato-3 (BT-3). Prior to the establishment of transformation protocol cotyledonary leaf explants from the two varieties were cultured to obtain genotype independent in vitro regeneration. Healthy multiple shoot regeneration was obtained from the cut ends of cotyledonary leaf segments for both the varieties on MS containing 1.0 mg/l BAP and 0.1 mg/l IAA. The maximum root induction from the regenerated shoots was achieved on half the strength of MS medium supplemented with 0.2 mg/l IAA. The in vitro grown plantlets were successfully transplanted into soil where they flowered and produced fruits identical to those developed by control plants. Transformation ability of cotyledonary leaf explants was tested with Agrobacterium tumefaciens strain LBA4404 harboring binary plasmid pBI121, containing GUS and npt II genes. Transformed cotyledonary leaf explants were found to produce multiple shoots on MS containing 1.0 mg/l BAP and 0.1 mg/l IAA. Selection of the transformed shoots was carried out by gradually increasing the concentration of kanamycin to 200 mg/l since kanamycin resistant gene was used for transformation experiments. Shoots that survived under selection pressure were subjected to rooting. Transformed rooted plantlets were transferred to soil. Stable expression of GUS gene was detected in the various tissues from putatively transformed plantlets using GUS histochemical assay.  Key words: In vitro regeneration, transformation, tomato D.O.I. 10.3329/ptcb.v19i1.5004 Plant Tissue Cult. & Biotech. 19(1): 101-111, 2009 (June)

scholarly journals Nutrient optimization for improved in vitro plant regeneration in Eclipta alba (L.) Hassk. and assessment of genetic fidelity using RAPD analysis

2015 ◽  
Vol 24 (2) ◽  
pp. 223-234
Author(s):  
Shruti Bardar ◽  
Varsha Khurana Kaul ◽  
Sumita Kachhwaha ◽  
SL Kothari
Keyword(s):  
Basal Medium ◽  
Genetic Fidelity ◽  
Ex Situ ◽  
Nutrient Level ◽  
Induction Medium ◽  
Eclipta Alba ◽  
Shoot Bud ◽  
Bud Induction ◽  
Regeneration Response

This study highlights the effect of different inorganic micronutrients like copper, cobalt, molybdenum, zinc, boron, iodine, iron and manganese in accelerating and amplifying in vitro shoot bud induction and proliferation of a medicinally important plant, Eclipta alba (L.) Hassk. Direct shoot bud induction was observed on MS fortified with Kn (2 mg/l). However, maximum number of shoots was achieved when GA3, 0.5 mg/l was added to induction medium along with 1?M copper sulphate (ten times the normal MS level). Optimization of nutrient level in the basal medium promoted maximum regeneration response from both shoot tips and nodal explants. Elongated shoots were rooted in MS supplemented with IBA, 1.0 mg/l. Healthy, green plantlets with well developed roots, flowered normally in the field. Genetic stability of micropropagated plantlets was evaluated using RAPD markers. The amplification products were monomorphic in micropropagated plantlets and similar to those of mother plant revealing the genetic uniformity of plantlets. The regeneration protocol is highly efficient and reproducible so would be useful for mass multiplication, ex situ conservation and genetic transformation of E. alba (L.) Hassk.Plant Tissue Cult. & Biotech. 24(2): 223-234, 2014 (December)

scholarly journals In vitro conservation protocol of Ceropegia bulbosa: An important medicinal and threatened plant species of Western Rajasthan

2017 ◽  
Vol 4 (1) ◽  
pp. 21 ◽  
Author(s):  
Suman Parihar
Keyword(s):  
In Vitro Regeneration ◽  
Axillary Shoot ◽  
Shoot Bud ◽  
Adenine Sulphate ◽  
Bud Induction ◽  
Threatened Plant Species ◽  
Morphogenic Response ◽  
House Condition ◽  
Ceropegia Bulbosa

In vitro regeneration protocol has been standardized for highly medicinal and threatened succulent Ceropegia bulbosa Roxb. The paper focuses on morphogenic response of nodal explant when cultured on MS media. Murashige and Skoog medium supplemented with 6-benzyladenine (BA) (2.0 mgl-1) was found optimum for axillary shoot bud induction with 83.4 % response. Further shoots were multiplied through repetitive (3-4 times) transfer of the original explant and by subculture of the in vitro generated shoots. Maximum number of shoots 5.7±0.78 with shoot length of 3.6±0.82 cm was achieved on MS medium augmented with combination of 0.25 mgl-1 BA + 0.25 mgl-1 KN + 0.1 mgl-1 IAA and additives (50.0 mgl-1 ascorbic acid, 25 mgl-1 each of citric acid, arginine and adenine sulphate). For ex vitro rooting, pulse treatment of IBA 250 mgl-1 for 3 min was found optimum. The rooted shoots were successfully hardened in the green house condition (RH 75-80% at 26-28˚C) and about 80 % shoots were transferred to the garden.

scholarly journals In vitro plant regeneration in rough lemon (Citrus jambhiri Lush.) through epicotyl segments by direct shoot organogenesis

2016 ◽  
Vol 8 (2) ◽  
pp. 724-729
Author(s):  
Sukhjit Kaur
Keyword(s):  
In Vitro Regeneration ◽  
Growth Hormones ◽  
Garden Soil ◽  
Direct Regeneration ◽  
Ms Medium ◽  
Citrus Jambhiri ◽  
Rough Lemon ◽  
Induction Frequency ◽  
Bud Induction

The effect of Murashige and Skoog (MS) medium supplemented with various concentrations and combinations of growth hormones on direct regeneration from one month old epicotyl segments of in vitro grown rough lemon (Citrus jambhiri Lush.) seedlings was studied. The earliest bud induction in 7.5 days, highest bud induction frequency (98.50%), percent regeneration(90.53) were obtained on MS medium supplemented with 6-Benzylaminopurine (BAP) (1mglit-1) with an average number of 12.50 buds per explants. The epicotyls segments with proliferated buds were transferred to elongation media in order to improve the recovery of normal shoots. Maximum number of elongated shoots (8.50) was obtained on MS medium having BAP (0.5mglit-1) + Gibberellic Acid (GA3)(1.0 mglit-1).These elongated shoots were then rooted on MS medium containing Indole-3-butyric acid (IBA) (0.1mglit-1) + Indole-3-aceticacid(IAA)(0.5mglit-1) with highest rooting percentage(96%) and root number(5.0). Early (10.10 days) rooting was observed in MS medium supplemented with NAA1.0 mglit-1 + IBA0.5 mglit-1.The plantlet survival was 98.52%, when plantlets were transferred to plastic pots containing a mixture of garden soil and vermiculite (1:1). The hardened plants were successfully established in the soil. The present study developed protocol which can be reliably used for in vitro regeneration of rough lemon and for gene transfer studies in rough lemon, especially to induce salinity and Phytophthora tolerance.

scholarly journals A Novel Method for Efficient Micropropagation of Radermachera xylocarpa (Roxb.) K. Schum., a Rare Medicinally Important Forest Species

2013 ◽  
Vol 23 (1) ◽  
Author(s):  
Manjary Sathe ◽  
Megha Vibhute ◽  
Monica Jain ◽  
Pankaj Srivastav
Keyword(s):  
Tree Species ◽  
Large Scale ◽  
In Vitro Regeneration ◽  
Forest Tree ◽  
Root Induction ◽  
Mass Propagation ◽  
Forest Tree Species ◽  
Bud Induction ◽  
Indigenous Forest

Radermachera xylocarpa (Roxb.) K. Schum. is a rare indigenous forest tree species which is utilized for its wood and medicinal properties. Due to its overexploitation and specific habitat requirements the species is restricted to limited areas. In vitro mass propagation of tree species faces various challenges and no such efforts have yet been taken in propagation of this useful plant using these methods.  In order to overcome the hurdles and understanding an urgent need of its conservation and mass propagation present authors attempt to develop a simple effective tissue culture protocol for regeneration of R. xylocarpa. Nodal explants were cultured on MS supplemented with various concentrations of cytokinins and auxins.  Among different cytokinins, maximum bud induction and proliferation was obtained in media supplemented with Kn along with IBA and for effective root induction which is tough to obtain in tree species, 100% rooting was achieved in cultures with increasing concentrations of IBA. Field survival is a major challenge with regenerated plants of forest tree species. We report here for the first time 100% survival of plants in soil by carefully standardizing the period of hardening and acclimatization procedures. A novel and effective in vitro regeneration protocol of R. xylocarpa has been successfully standardized which can be adopted for large scale propagation, reforestation and conservation of rare Radermachera xylocarpa of medicinal importance.Plant Tissue Cult. & Biotech. 23(1): 21?29, 2013 (June)DOI: http://dx.doi.org/10.3329/ptcb.v23i1.15556

scholarly journals High Frequency In vitro Regeneration of Gynura procumbens (Lour.) Merr.

2017 ◽  
Vol 27 (2) ◽  
pp. 207-216
Author(s):  
Tanjina Akhtar Banu ◽  
Barna Goswami ◽  
Shahina Akter ◽  
Mousona Islam ◽  
Tammana Tanjin ◽  
...  
Keyword(s):  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Root Induction ◽  
Petiole Explants ◽  
Number Of Shoots

An efficient rapid in vitro regeneration protocol was described from nodal segment, leaf and petiole explants. MS medium supplemented with 1.0 mg/l BAP and 0.5 mg/l IAA was found best for the multiple shoot formation from nodal segments. In this combination 99% explants produced multiple shoots and the average number of shoots per explants was 20.1 ± 1.96. For petiole and leaf explants best response was observed on MS supplemented with 2.0 mg/l BAP, 1 mg/l IAA and 0.5 mg/l Kn. Petiole explants produced highest mean number of shoots/explant (22.9 ± 1.728) among the three explants when the explants were cultured on MS with 2.0 mg/l BAP, 1 mg/l IAA and 0.5 mg/l Kn. The highest frequency of root induction (100%) and mean number of roots/plantlets (11.75) were obtained on MS. The rooted plantlets were transferred for hardening following acclimatization and finally were successfully established in the field.Plant Tissue Cult. & Biotech. 27(2): 207-216, 2017 (December)

Sign in / Sign up

Export Citation Format

Share Document