scholarly journals Performance Comparison of Five SARS-CoV-2 Antibody Assays for Seroprevalence Studies

2022 ◽  
Vol 42 (1) ◽  
pp. 71-78
Author(s):  
Younhee Park ◽  
Ki Ho Hong ◽  
Su-Kyung Lee ◽  
Jungwon Hyun ◽  
Eun-Jee Oh ◽  
...  
Author(s):  
Kimberly J Paiva ◽  
Ricky D Grisson ◽  
Philip A Chan ◽  
John R. Lonks ◽  
Ewa King ◽  
...  

AbstractSerology testing of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is increasingly being used during the current pandemic of Coronavirus Disease 2019 (COVID-19). The clinical and epidemiologic utilities of antibody-based SARS-CoV-2 testing are under debate. Characterizing these assays helps to understand the disease and provides scientific basis for deciding how to best use these assays. The study assessed one chemiluminescent assay (Abbott COVID-2 IgG) and two lateral flow assays (STANDARD Q [SQ] IgM/IgG Duo and Wondfo Total Antibody Test). Validation included 113 blood samples from 71 PCR-confirmed COVID-19 patients and 1182 samples from negative controls with potential interferences/cross-reactions, including 1063 pre-pandemic samples. IgM antibodies against SARS-CoV-2 were detected as early as post-symptom onset days 3-4. IgG antibodies were first detected post-onset days 5-6 by SQ assays. The detection rates increased gradually, and SQ IgG, Abbott IgG and Wondfo Total detected antibodies from all the PCR-confirmed patients 14 days after symptom onset. Overall agreements between SQ IgM/IgG and Wondfo Total was 88.5% and between SQ IgG and Abbott IgG was 94.6% (Kappa = 0.75, 0.89). No cross-reaction with other endemic coronavirus infections were identified. Viral hepatitis and autoimmune samples were the main cross-reactions observed. However, the interferences/cross-reactions were low. The specificities were 100% for SQ IgG and Wondfo Total and 99.62% for Abbott IgG and 98.87% for SQ IgM. These findings demonstrate high sensitivity and specificity of appropriately validated antibody-based SARS-CoV-2 assays with implications for clinical use and epidemiological seroprevalence studies.


2020 ◽  
Vol 93 (2) ◽  
pp. 916-923 ◽  
Author(s):  
Kimberly J. Paiva ◽  
Ricky D. Grisson ◽  
Philip A. Chan ◽  
Richard C. Huard ◽  
Angela M. Caliendo ◽  
...  

Author(s):  
Molla Asmare ◽  
Mustafa Ilbas

Nowadays, the most decisive challenges we are fronting are perfectly clean energy making for equitable and sustainable modern energy access, and battling the emerging alteration of the climate. This is because, carbon-rich fuels are the fundamental supply of utilized energy for strengthening human society, and it will be sustained in the near future. In connection with this, electrochemical technologies are an emerging and domineering tool for efficiently transforming the existing scarce fossil fuels and renewable energy sources into electric power with a trivial environmental impact. Compared with conventional power generation technologies, SOFC that operate at high temperature is emerging as a frontrunner to convert the fuels chemical energy into electric power and permits the deployment of varieties of fuels with negligible ecological destructions. According to this critical review, direct ammonia is obtained as a primary possible choice and price-effective green fuel for T-SOFCs. This is because T-SOFCs have higher volumetric power density, mechanically stable, and high thermal shocking resistance. Also, there is no sealing issue problem which is the chronic issues of the planar one. As a result, the toxicity of ammonia to use as a fuel is minimized if there may be a leakage during operation. It is portable and manageable that can be work everywhere when there is energy demand. Besides, manufacturing, onboard hydrogen deposition, and transportation infrastructure connected snags of hydrogen will be solved using ammonia. Ammonia is a low-priced carbon-neutral source of energy and has more stored volumetric energy compared with hydrogen. Yet, to utilize direct NH3 as a means of hydrogen carrier and an alternative green fuel in T-SOFCs practically determining the optimum operating temperatures, reactant flow rates, electrode porosities, pressure, the position of the anode, thickness and diameters of the tube are still requiring further improvement. Therefore, mathematical modeling ought to be developed to determine these parameters before planning for experimental work. Also, a performance comparison of AS, ES, and CS- T-SOFC powered with direct NH3 will be investigated and best-performed support will be carefully chosen for practical implementation and an experimental study will be conducted for verification based on optimum parameter values obtained from numerical modeling.


1999 ◽  
Author(s):  
C-C. Chen ◽  
C. Lai ◽  
T. Shih

2004 ◽  
Author(s):  
C. Chen ◽  
K. Chang ◽  
T. Lin ◽  
C. Hsu ◽  
Y. Lee ◽  
...  

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