scholarly journals Preliminary evaluation of Brazilian green propolis as coadjuvant of a subunit vaccine against lethal leptospirosis

2021 ◽  
Vol 10 (12) ◽  
pp. e521101220945
Author(s):  
Michel Quevedo Fagundes ◽  
Amilton Clair Pinto Seixas Neto ◽  
Samuel Rodrigues Felix ◽  
Flávia Aleixo Vasconcellos ◽  
Geferson Fischer ◽  
...  
Keyword(s):  
Immune Response ◽  
Immune Modulation ◽  
Protective Immune Response ◽  
Control Measures ◽  
Optimum Dose ◽  
Preliminary Evaluation ◽  
Whole Cell ◽  
Molecular Assays ◽  
Heterologous Challenge ◽  
A Subunit

Vaccination would be preferred to leptospirosis control measures. However, current vaccines are heat killed whole-cell bacterins that generate serovar specific protection and several side effects. Modern molecular assays have revealed antigens that may replace traditional whole-cell vaccines. Among these, LigB protein is surface-exposed outer membrane protein of virulent leptospires and therefore potential target of a protective immune response. Some unsuccessful attempts at using these antigens as vaccines have been reported. However, we believe that immune modulation through alternative adjuvants and co-adjuvants may overcome previous setbacks. In this light, our study aimed to evaluate the protective immune response in hamsters vaccinated with 40 µg of rLigBNI using oil adjuvant (OA), with or without green propolis (GP) as co-adjuvant. Upon a challenge, all groups immunized with rLigBNI, coupled or not with GP, were highly immunogenic and revealed statistically significant (p<0.05) protection of hamsters from lethal leptospirosis. Additional studies are being carried out to assess the optimum dose, protection against heterologous challenge, and vaccine dynamics.

Variations in the protective immune response against streptococcal superantigens in populations of different ethnicity

2005 ◽  
Vol 195 (1) ◽  
pp. 37-43 ◽  
Author(s):  
Lily P. H. Yang ◽  
Björn K. G. Eriksson ◽  
Zinta Harrington ◽  
Nigel Curtis ◽  
Selwyn Lang ◽  
...  
Keyword(s):  
Immune Response ◽  
Protective Immune Response

Immunomodulation in host-protective immune response against murine tuberculosis through regulation of the T regulatory cell function

2015 ◽  
Vol 98 (5) ◽  
pp. 827-836 ◽  
Author(s):  
S. Das ◽  
K. Halder ◽  
A. Goswami ◽  
B. P. Chowdhury ◽  
N. K. Pal ◽  
...  
Keyword(s):  
Immune Response ◽  
Cell Function ◽  
Protective Immune Response ◽  
T Regulatory Cell ◽  
Regulatory Cell

Recombinant Glycoprotein B of Equine herpesvirus Type 1 Elicits Protective Immune Response against Challenge in BALB/c Mouse Model

2021 ◽  
Author(s):  
Alok Joshi ◽  
R.P. Gupta ◽  
Selvaraj Pavulraj ◽  
Bidhan Chandra Bera ◽  
Taruna Anand ◽  
...  
Keyword(s):  
Immune Response ◽  
Plasmid Dna ◽  
Protective Immune Response ◽  
Glycoprotein B ◽  
Equine Herpesvirus ◽  
Recombinant Glycoprotein ◽  
Herpesvirus Type ◽  
Equine Herpesvirus Type ◽  
Equine Herpesvirus Type 1

Background: Equine herpesvirus type 1 (EHV-1) is the most important viral pathogen of equines, causing respiratory illness, abortion, neonatal foal mortality and neurologic disorders. Large numbers of commercial EHV-1 vaccines are available to protect equines from the disease, but they provide only partial protection. Despite immunization with inactivated and modified live virus vaccine, mares show abortions. Present study was aimed to investigate the immunogenicity and protective efficacy of EHV-1 recombinant glycoprotein B (rgB) and gB expressing plasmid DNA against EHV-1 infection in BALB/c mice model.Methods: About 3-4 weeks old 225 female BALB/c mice were selected for the comparative study of immunization followed by challenged with EHV-1/India/Tohana/96-2 strain virus in 5 different groups of 45 animals each.Result: Following immunization, rgB vaccinated mice showed optimal stimulation of EHV-1 gB specific cell mediated and humoral mediated immunity (HMI and CMI). The gB expressing plasmid DNA vaccinated mice developed only CMI while inactivated whole virus vaccinated mice had only HMI. Upon EHV-1 challenge, all infected mice displayed variable levels of clinical signs with changes in body weight, however, vaccinated mice showed very rapid recovery with optimal protection. Positive control group mice showed severe pulmonary lesions along with persistence virus infection till 5 days post challenge (dpc) whereas vaccinated mice had less pulmonary lesion only up to 3dpc. Minimal lung lesions and early virus clearance was observed in the rgB immunized mice in comparison to the gB plasmid DNA and inactivated EHV-1 vaccine immunized mice. It has been concluded that immunization with rgB elicits optimum protective immune response against EHV-1 infection in mice model. The rgB could be a potential vaccine candidate against EHV-1 infection in equine in the future.

scholarly journals Metformin exerts antitumor activity via induction of multiple death pathways in tumor cells and activation of a protective immune response

Oncotarget ◽  
2018 ◽  
Vol 9 (40) ◽  
pp. 25808-25825 ◽  
Author(s):  
Felipe V. Pereira ◽  
Amanda Campelo L. Melo ◽  
Jun Siong Low ◽  
Íris Arantes de Castro ◽  
Tárcio T. Braga ◽  
...  
Keyword(s):  
Immune Response ◽  
Antitumor Activity ◽  
Tumor Cells ◽  
Protective Immune Response
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