scholarly journals Comparison of the Real-time Nucleic Acid Sequence-based Amplification (RTi-NASBA) with Conventional NASBA, and Galactomannan Assay for the Diagnosis of Invasive Aspergillosis

2007 ◽  
Vol 22 (4) ◽  
pp. 672 ◽  
Author(s):  
Jin-Hong Yoo ◽  
Su-Mi Choi ◽  
Dong-Gun Lee ◽  
Sun-Hee Park ◽  
Jung-Hyun Choi ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Invasive Aspergillosis ◽  
Real Time ◽  
Nucleic Acid Sequence ◽  
The Real

scholarly journals Real-Time Nucleic Acid Sequence-Based Amplification Assay for Detection of Hepatitis A Virus

2005 ◽  
Vol 71 (11) ◽  
pp. 7113-7116 ◽  
Author(s):  
Khaled H. Abd El Galil ◽  
M. A. El Sokkary ◽  
S. M. Kheira ◽  
Andre M. Salazar ◽  
Marylynn V. Yates ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Hepatitis A ◽  
Molecular Beacon ◽  
Hepatitis A Virus ◽  
Immunomagnetic Separation ◽  
Nucleic Acid Sequence ◽  
The Real ◽  
Nasba Assay ◽  
Environmental Pathogens

ABSTRACT A nucleic acid sequence-based amplification (NASBA) assay in combination with a molecular beacon was developed for the real-time detection and quantification of hepatitis A virus (HAV). A 202-bp, highly conserved 5′ noncoding region of HAV was targeted. The sensitivity of the real-time NASBA assay was tested with 10-fold dilutions of viral RNA, and a detection limit of 1 PFU was obtained. The specificity of the assay was demonstrated by testing with other environmental pathogens and indicator microorganisms, with only HAV positively identified. When combined with immunomagnetic separation, the NASBA assay successfully detected as few as 10 PFU from seeded lake water samples. Due to its isothermal nature, its speed, and its similar sensitivity compared to the real-time RT-PCR assay, this newly reported real-time NASBA method will have broad applications for the rapid detection of HAV in contaminated food or water.

scholarly journals Real-Time Nucleic Acid Sequence-Based Amplification to Predict the Clinical Outcome of Invasive Aspergillosis

2012 ◽  
Vol 27 (1) ◽  
pp. 10 ◽  
Author(s):  
Si-Hyun Kim ◽  
Chulmin Park ◽  
Eun-Young Kwon ◽  
Na-Young Shin ◽  
Jae-Cheol Kwon ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Clinical Outcome ◽  
Invasive Aspergillosis ◽  
Real Time ◽  
Nucleic Acid Sequence

Rapid and simultaneous detection of three major diarrhea-causing viruses by multiplex real-time nucleic acid sequence-based amplification

2015 ◽  
Vol 160 (3) ◽  
pp. 719-725 ◽  
Author(s):  
Qiu-Hua Mo ◽  
Hai-Bo Wang ◽  
Hui-Rong Dai ◽  
Ji-Can Lin ◽  
Hua Tan ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Simultaneous Detection ◽  
Nucleic Acid Sequence

scholarly journals Comparison and Evaluation of Real-Time PCR, Real-Time Nucleic Acid Sequence-Based Amplification, Conventional PCR, and Serology for Diagnosis of Mycoplasma pneumoniae

2003 ◽  
Vol 41 (9) ◽  
pp. 4366-4371 ◽  
Author(s):  
K. E. Templeton ◽  
S. A. Scheltinga ◽  
A. W. Graffelman ◽  
J. M. van Schie ◽  
J. W. Crielaard ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Mycoplasma Pneumoniae ◽  
Real Time Pcr ◽  
Nucleic Acid Sequence ◽  
Conventional Pcr

scholarly journals Detection and Quantification of the Red Tide Dinoflagellate Karenia brevis by Real-Time Nucleic Acid Sequence-Based Amplification

2004 ◽  
Vol 70 (8) ◽  
pp. 4727-4732 ◽  
Author(s):  
Erica T. Casper ◽  
John H. Paul ◽  
Matthew C. Smith ◽  
Michael Gray
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Wildlife Conservation ◽  
Red Tide ◽  
Diagnostic Testing ◽  
Karenia Brevis ◽  
Nucleic Acid Sequence ◽  
Cell Counts ◽  
Wide Range

ABSTRACT Nucleic acid sequence-based amplification (NASBA) is an isothermal method of RNA amplification that has been previously used in clinical diagnostic testing. A real-time NASBA assay has been developed for the detection of rbcL mRNA from the red tide dinoflagellate Karenia brevis. This assay is sensitive to one K. brevis cell and 1.0 fg of in vitro transcript, with occasional detection of lower concentrations of transcript. The assay did not detect rbcL mRNA from a wide range of nontarget organisms and environmental clones, while 10 strains (all tested) of K. brevis were detected. By the use of standard curves based on time to positivity, concentrations of K. brevis in environmental samples were predicted by NASBA and classified into different levels of blooms per the Florida Fish and Wildlife Conservation Commission (FWC) system. NASBA classification matched FWC classification (based on cell counts) 72% of the time. Those samples that did not match were off by only one class. NASBA is sensitive, rapid, and effective and may be used as an additional or alternative method to detect and quantify K. brevis in the marine environment.

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