Rapid quantitative detection of chytridiomycosis (Batrachochytrium dendrobatidis) in amphibian samples using real-time Taqman PCR assay

Abstract A non-radio-labeled probe-based detection method was developed for rapid enumeration of Salmonella in seafood and water samples. A Salmonella-specific invA gene probe was developed using a digoxigenin-based non-radio labeling assay, which was evaluated with naturally contaminated seafood and water samples. The probe-based technique was further compared with the quantitative PCR assay. The method was specific for detection of different Salmonella serovars without any nonspecific hybridization with other Salmonella-related Enterobacteriaceae. The optimum labeling efficiency was determined for the labeled probe, and 10 pg/μL probe concentration was observed to be most efficient for detection of Salmonella colonies on nylon membrane. Quantification of Salmonella in naturally contaminated seafood and water samples (n = 21) was in the range 10–102 CFU/mL. The assay successfully quantified Salmonella in spiked seafood and water samples in the presence of background flora, and the entire assay was completed within 48 h. The probe-based assay was further evaluated with real-time PCR, and results showed that the assay was comparable to real-time PCR assay. Thus, this probe-based assay can be a rapid, useful, and alternative technique for quantitative detection of Salmonella in food, feed, and water samples.

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Quantitative detection of hepatitis C virus RNA in urine of patients with chronic hepatitis C using a novel real‐time PCR assay

Journal of Medical Virology ◽

10.1002/jmv.25280 ◽

2018 ◽

Vol 91 (1) ◽

pp. 115-123 ◽

Cited By ~ 2

Author(s):

Tian Lu ◽

Yanxi Han ◽

Rui Zhang ◽

Kuo Zhang ◽

Guigao Lin ◽

...

Keyword(s):

Chronic Hepatitis ◽

Hepatitis C Virus ◽

Hepatitis C ◽

Real Time ◽

Chronic Hepatitis C ◽

Real Time Pcr ◽

Quantitative Detection ◽

Hepatitis C Virus Rna ◽

Pcr Assay ◽

Virus Rna

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Development and validation of a SYBR Green real-time PCR assay for rapid and quantitative detection of goose interferons and proinflammatory cytokines

Poultry Science ◽

10.3382/ps/pev241 ◽

2015 ◽

Vol 94 (10) ◽

pp. 2382-2387 ◽

Cited By ~ 5

Author(s):

Hao Zhou ◽

Shun Chen ◽

Yulin Qi ◽

Mingshu Wang ◽

Renyong Jia ◽

...

Keyword(s):

Real Time ◽

Proinflammatory Cytokines ◽

Real Time Pcr ◽

Sybr Green ◽

Quantitative Detection ◽

Pcr Assay ◽

Development And Validation

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Fast and sensitive quantitative detection of HIV DNA in whole blood leucocytes by SYBR green I real-time PCR assay

Molecular and Cellular Probes ◽

10.1016/j.mcp.2007.05.005 ◽

2007 ◽

Vol 21 (5-6) ◽

pp. 368-378 ◽

Cited By ~ 27

Author(s):

Anna Casabianca ◽

Caterina Gori ◽

Chiara Orlandi ◽

Federica Forbici ◽

Carlo Federico Perno ◽

...

Keyword(s):

Real Time ◽

Real Time Pcr ◽

Whole Blood ◽

Sybr Green ◽

Sybr Green I ◽

Quantitative Detection ◽

Pcr Assay ◽

Hiv Dna

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Real-time TaqMan PCR for rapid detection and typing of genes encoding CTX-M extended-spectrum β-lactamases

Journal of Medical Microbiology ◽

10.1099/jmm.0.46909-0 ◽

2007 ◽

Vol 56 (1) ◽

pp. 52-55 ◽

Cited By ~ 94

Author(s):

Christopher I. Birkett ◽

Hugo A. Ludlam ◽

Neil Woodford ◽

Derek F. J. Brown ◽

Nicholas M. Brown ◽

...

Keyword(s):

Real Time ◽

Taqman Assay ◽

Esbl Production ◽

Pcr Assay ◽

Extended Spectrum ◽

Genes Encoding ◽

Taqman Pcr ◽

Assay Time ◽

Phenotypic Methods ◽

Single Reaction

The prevalence of CTX-M-producing members of the Enterobacteriaceae is increasing worldwide. A novel, multiplex, real-time TaqMan PCR assay to detect and type bla CTX-M genes is described which is an improvement on previously described techniques with respect to reduced assay time, elimination of the need for protracted post-PCR processing and the convenience of a single reaction vessel. Based on β-lactam antibiogram and MIC data, 478 of 1279 Enterobacteriaceae isolates from clinical blood and urine culture specimens were selected and tested for extended-spectrum β-lactamase (ESBL) production using phenotypic methods. The new TaqMan assay detected and typed bla CTX-M genes in 21 of 28 ESBL-producing isolates.

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