scholarly journals Development of a Multitarget Real-Time TaqMan PCR Assay for Enhanced Detection of Francisella tularensis in Complex Specimens

2003 ◽  
Vol 41 (12) ◽  
pp. 5492-5499 ◽  
Author(s):  
J. L. Versage ◽  
D. D. M. Severin ◽  
M. C. Chu ◽  
J. M. Petersen
Keyword(s):  
Real Time ◽  
Francisella Tularensis ◽  
Pcr Assay ◽  
Taqman Pcr

Differentiation of herpes simplex virus types 1 and 2 in clinical samples by a real-time taqman PCR assay

2005 ◽  
Vol 76 (3) ◽  
pp. 350-355 ◽  
Author(s):  
Lawrence Corey ◽  
Meei-Li Huang ◽  
Stacy Selke ◽  
Anna Wald
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Real Time ◽  
Clinical Samples ◽  
Pcr Assay ◽  
Taqman Pcr ◽  
Simplex Virus

scholarly journals Real-time TaqMan PCR for rapid detection and typing of genes encoding CTX-M extended-spectrum β-lactamases

2007 ◽  
Vol 56 (1) ◽  
pp. 52-55 ◽  
Author(s):  
Christopher I. Birkett ◽  
Hugo A. Ludlam ◽  
Neil Woodford ◽  
Derek F. J. Brown ◽  
Nicholas M. Brown ◽  
...  
Keyword(s):  
Real Time ◽  
Taqman Assay ◽  
Esbl Production ◽  
Pcr Assay ◽  
Extended Spectrum ◽  
Genes Encoding ◽  
Taqman Pcr ◽  
Assay Time ◽  
Phenotypic Methods ◽  
Single Reaction

The prevalence of CTX-M-producing members of the Enterobacteriaceae is increasing worldwide. A novel, multiplex, real-time TaqMan PCR assay to detect and type bla CTX-M genes is described which is an improvement on previously described techniques with respect to reduced assay time, elimination of the need for protracted post-PCR processing and the convenience of a single reaction vessel. Based on β-lactam antibiogram and MIC data, 478 of 1279 Enterobacteriaceae isolates from clinical blood and urine culture specimens were selected and tested for extended-spectrum β-lactamase (ESBL) production using phenotypic methods. The new TaqMan assay detected and typed bla CTX-M genes in 21 of 28 ESBL-producing isolates.

scholarly journals Quantitative Assessment of the Tetracycline Resistance Gene Pool in Cheese Samples by Real-Time TaqMan PCR

2007 ◽  
Vol 73 (5) ◽  
pp. 1676-1677 ◽  
Author(s):  
Michele Y. Manuzon ◽  
Scott E. Hanna ◽  
Hongliang Luo ◽  
Zhongtang Yu ◽  
W. James Harper ◽  
...  
Keyword(s):  
Real Time ◽  
Gene Pool ◽  
Antibiotic Resistance Genes ◽  
Tetracycline Resistance ◽  
Food Samples ◽  
Independent Method ◽  
Pcr Assay ◽  
Tetracycline Resistance Gene ◽  
Culture Independent ◽  
Taqman Pcr

ABSTRACT A TaqMan real-time PCR assay was developed to quantify the tetS gene pool present in retail cheeses. This protocol offers a rapid, specific, sensitive, and culture-independent method for assessing antibiotic resistance genes in food samples rich in fats and proteins.

Development and validation of a real-time TaqMan PCR assay for the detection of betanodavirus in clinical specimens

2010 ◽  
Vol 155 (8) ◽  
pp. 1193-1203 ◽  
Author(s):  
V. Panzarin ◽  
P. Patarnello ◽  
A. Mori ◽  
E. Rampazzo ◽  
E. Cappellozza ◽  
...  
Keyword(s):  
Real Time ◽  
Pcr Assay ◽  
Clinical Specimens ◽  
Taqman Pcr ◽  
Development And Validation

A real-time TaqMan PCR assay to discriminate between pathotype 1 (D1) and non-pathotype 1 (D1) isolates of Synchytrium endobioticum

2015 ◽  
Vol 143 (3) ◽  
pp. 495-506 ◽  
Author(s):  
Peter J. M. Bonants ◽  
Marga P. E. van Gent-Pelzer ◽  
Gerard C. M. van Leeuwen ◽  
Theo A. J. van der Lee
Keyword(s):  
Real Time ◽  
Pcr Assay ◽  
Synchytrium Endobioticum ◽  
Taqman Pcr
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