scholarly journals Production of haploids and double haploids in annual (Lolium multiflorum) and perennial (L. perenne) ryegrasses

2006 ◽  
Vol 12 ◽  
pp. 45-48
Author(s):  
Syed Wajid Hussain ◽  
Kim Richardson ◽  
Marty Faville ◽  
Derek Woodfield
Keyword(s):  
Perennial Ryegrass ◽  
Simple Sequence Repeat ◽  
Lolium Multiflorum ◽  
Annual Ryegrass ◽  
Sequence Repeat ◽  
Homozygous Condition ◽  
Green Plants ◽  
Double Haploids ◽  
Haploid Pollen ◽  
Simple Sequence

Anthers from 32 genotypes from one line of annual ryegrass (Lolium multiflorum) and 229 genotypes from 15 cultivars of perennial ryegrass (L. perenne) were tested for the production of haploids and double haploids. Six (23%) annual and 71 (31%) perennial ryegrass genotypes produced calli ranging from 1.3-16% and 0.8- 12.2% respectively. However none of the annual ryegrass genotypes produced green plants and only one genotype (Option WH-1) of perennial ryegrass produced 16 (1.8%) green plants. The remaining produced only albino regenerates. Twelve of the 16 green plants were evaluated cytologically for chromosome numbers. Four were haploids thus confirming their origin from haploid pollen. The remaining eight were all diploids. One haploid and one diploid were tested for their homozygous condition using seven simple sequence repeat (SSR) markers and were found to be homozygous at all seven loci. In order to increase the recovery of green regenerates, we have used the responsive genotype Option WH-1 in crosses with three other perennial ryegrass genotypes and the progenies are currently been grown for anther culture

scholarly journals Comparative Analysis of Genetic Similarity between Perennial Ryegrass Genotypes Investigated With AFLPs, ISSRs, RAPDs and SSRs

2011 ◽  
Vol 42 (No. 3) ◽  
pp. 87-94 ◽  
Author(s):  
U.K. Posselt ◽  
P. Barre ◽  
G. Brazauskas ◽  
L.B. Turner
Keyword(s):  
Perennial Ryegrass ◽  
Simple Sequence Repeat ◽  
Fragment Length Polymorphism ◽  
Genetic Relationships ◽  
Random Amplified Polymorphic Dna ◽  
Correlation Coefficients ◽  
Inter Simple Sequence Repeat ◽  
Grass Species ◽  
Sequence Repeat ◽  
Simple Sequence

Perennial ryegrass (Lolium perenne L.) is the most important grass species used in temperate grassland agriculture. Our objective was to obtain an overview of the genetic relationships between 20 individual genotypes of perennial ryegrass of diverse origins, using amplified fragment length polymorphism (AFLP), inter-simple sequence repeat (ISSR), random amplified polymorphic DNA (RAPD) and two sets of simple sequence repeat (SSR) markers. All 20 individuals were uniquely fingerprinted by all four marker systems and comparisons were made on the basis of 85 markers each. Mean genetic similarities were estimated at 0.31, 0.43, 0.23 and 0.15 for AFLPs, ISSRs, RAPDs and SSRs, respectively. Cophenetic values resulted in good (AFLP and SSR-B = 0.88) to moderately good fits (ISSR = 0.76, RAPD = 0.70, and SSR-A = 0.79). Comparing the four marker systems to each other, AFLP and SSR-A were correlated best (r = 0.57). All other comparisons revealed rather low correlation coefficients in the Mantel Z test. With twice as many markers cophenetic values increased to a very good fit for AFLPs (0.90) and SSRs (0.92).      

Development and characterisation of simple sequence repeat (SSR) markers for perennial ryegrass (Lolium perenne L.)

2001 ◽  
Vol 102 (2-3) ◽  
pp. 405-415 ◽  
Author(s):  
E. S. Jones ◽  
M. P. Dupal ◽  
R. Kölliker ◽  
M. C. Drayton ◽  
J. W. Forster
Keyword(s):  
Ssr Markers ◽  
Lolium Perenne ◽  
Perennial Ryegrass ◽  
Simple Sequence Repeat ◽  
Sequence Repeat ◽  
Lolium Perenne L ◽  
Simple Sequence

scholarly journals Inter-simple sequence repeat (ISSR) loci mapping in the genome of perennial ryegrass

Biologija ◽  
2008 ◽  
Vol 54 (1) ◽  
pp. 17-21 ◽  
Author(s):  
O. Pivorienė ◽  
I. Pašakinskienė ◽  
G. Brazauskas ◽  
L. Lideikytė ◽  
L. B. Jensen ◽  
...  
Keyword(s):  
Perennial Ryegrass ◽  
Simple Sequence Repeat ◽  
Inter Simple Sequence Repeat ◽  
Sequence Repeat ◽  
Simple Sequence

A framework linkage map of perennial ryegrass based on SSR markers

Genome ◽  
2006 ◽  
Vol 49 (4) ◽  
pp. 354-364 ◽  
Author(s):  
G P Gill ◽  
P L Wilcox ◽  
D J Whittaker ◽  
R A Winz ◽  
P Bickerstaff ◽  
...  
Keyword(s):  
Linkage Map ◽  
Ssr Markers ◽  
Lolium Perenne ◽  
Perennial Ryegrass ◽  
Simple Sequence Repeat ◽  
Ssr Marker ◽  
Sequence Repeat ◽  
Linkage Groups ◽  
Marker Loci ◽  
Simple Sequence

A moderate-density linkage map for Lolium perenne L. has been constructed based on 376 simple sequence repeat (SSR) markers. Approximately one third (124) of the SSR markers were developed from GeneThresher® libraries that preferentially select genomic DNA clones from the gene-rich unmethylated portion of the genome. The remaining SSR marker loci were generated from either SSR-enriched genomic libraries (247) or ESTs (5). Forty-five percent of the GeneThresher SSRs were associated with an expressed gene. Unlike EST-derived SSR markers, GeneThresher SSRs were often associated with genes expressed at a low level, such as transcription factors. The map constructed here fulfills 2 definitions of a "framework map". Firstly, it is composed of codominant markers to ensure map transferability either within or among species. Secondly, it was constructed to achieve a level of statistical confidence in the support-for-order of marker loci. The map consists of 81 framework SSR markers spread over 7 linkage groups, the same as the haploid chromosome number. Most of the remaining 295 SSR markers have been placed into their most likely interval on the framework map. Nine RFLP markers and 1 SSR marker from another map constructed using the same pedigree were also incorporated to extend genome coverage at the terminal ends of 5 linkage groups. The final map provides a robust framework with which to conduct investigations into the genetic architecture of trait variation in this commercially important grass species.Key words: Framework map, perennial ryegrass, SSR, simple sequence repeat, GeneThresher, Lolium perenne.

Implementing molecular marker technology in forage improvement

2003 ◽  
pp. 229-238
Author(s):  
M. Faville ◽  
B. Barrett ◽  
A. Griffiths ◽  
M. Schreiber ◽  
C. Mercer ◽  
...  
Keyword(s):  
Quantitative Trait Locus ◽  
Genetic Variation ◽  
White Clover ◽  
Simple Sequence Repeat ◽  
Sequence Repeat ◽  
Linkage Groups ◽  
Seedling Vigour ◽  
Genome Map ◽  
Trait Locus ◽  
Simple Sequence

Accelerated improvement of two cornerstones of New Zealand's pastoral industries, per ennial ryegrass (Lolium perenne L.) and white clover (Trifolium repens L.), may be realised through the application of markerassisted selection (MAS) strategies to enhance traditional plant breeding programmes. Genome maps constructed using molecular markers represent the enabling technology for such strategies and we have assembled maps for each species using EST-SSR markers - simple sequence repeat (SSR) markers developed from expressed sequence tags (ESTs) representing genes. A comprehensive map of the white clover genome has been completed, with 464 EST-SSR and genomic SSR marker loci spanning 1125 cM in total, distributed across 16 linkage groups. These have been further classified into eight pairs of linkage groups, representing contributions from the diploid progenitors of this tetraploid species. In perennial ryegrass a genome map based exclusively on EST-SSR loci was constructed, with 130 loci currently mapped to seven linkage groups and covering a distance of 391 cM. This map continues to be expanded with the addition of ESTSSR loci, and markers are being concurrently transferred to other populations segregating for economically significant traits. We have initiated gene discovery through quantitative trait locus (QTL) analysis in both species, and the efficacy of the white clover map for this purpose was demonstrated with the initial identification of multiple QTL controlling seed yield and seedling vigour. One QTL on linkage group D2 accounts for 25.9% of the genetic variation for seed yield, and a putative QTL accounting for 12.7% of the genetic variation for seedling vigour was detected on linkage group E1. The application of MAS to forage breeding based on recurrent selection is discussed. Keywords: genome map, marker-assisted selection, perennial ryegrass, QTL, quantitative trait locus, SSR, simple sequence repeat, white clover

Genetic diversity of Symplocos paniculata of Hunan province revealed by inter-simple sequence repeat (ISSR)

2019 ◽  
Vol 51 (5) ◽  
Author(s):  
Huifang Cao ◽  
Qiang Lin ◽  
Peiwang Li ◽  
Jingzhen Chen ◽  
Changzhu Li ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Simple Sequence Repeat ◽  
Inter Simple Sequence Repeat ◽  
Hunan Province ◽  
Sequence Repeat ◽  
Simple Sequence
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