Preparation, Characterization and Preliminary <i>in vivo</i> Testing of Liposomated Antiidiotypic Antibodies Against Morphine Derivatives

Artiﬁcial membrane structures containing medicinal substances are highly promising for the development of new drugs. Liposomal preparations are actively used in medical practice due to their high efﬁcacy and relatively low toxicity. Our aim was to encapsulate anti-idiotypic antibodies into a liposomal composition with the purpose of improving their immunogenic properties. Following the preparation of a liposomal composition by the dehydration/rehydration method using ultrasonic treatment, the size, zeta potential, and loading efﬁciency of liposomes were investigated. Preliminary in vivo studies were conducted to evaluate the adjuvant properties of liposomes of varying size. Loaded liposomes of the smallest diameter (about 110 nm) showed the potential of enhancing the immune response similar to that obtained using Freund’s adjuvant. These results justify further research into the properties of liposomes loaded with antibodies.

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In Vivo Delivery of Silica Nanorattle Encapsulated Docetaxel for Liver Cancer Therapy with Low Toxicity and High Efficacy

ACS Nano ◽

10.1021/nn100918a ◽

2010 ◽

Vol 4 (11) ◽

pp. 6874-6882 ◽

Cited By ~ 246

Author(s):

Linlin Li ◽

Fangqiong Tang ◽

Huiyu Liu ◽

Tianlong Liu ◽

Nanjing Hao ◽

...

Keyword(s):

Cancer Therapy ◽

Liver Cancer ◽

High Efficacy ◽

Low Toxicity ◽

Silica Nanorattle ◽

In Vivo Delivery

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In vitro and in vivo anticancer activity of a novel puerarin nanosuspension against colon cancer, with high efficacy and low toxicity

International Journal of Pharmaceutics ◽

10.1016/j.ijpharm.2012.10.021 ◽

2013 ◽

Vol 441 (1-2) ◽

pp. 728-735 ◽

Cited By ~ 62

Author(s):

Yancai Wang ◽

Yingying Ma ◽

Ying Zheng ◽

Ju Song ◽

Xiao Yang ◽

...

Keyword(s):

Colon Cancer ◽

Anticancer Activity ◽

High Efficacy ◽

Low Toxicity

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In vitro evaluation of immunogenic properties of active dressings

Current Issues in Pharmacy and Medical Sciences ◽

10.2478/cipms-2014-0014 ◽

2014 ◽

Vol 27 (1) ◽

pp. 55-60 ◽

Cited By ~ 1

Author(s):

Joanna Orlowska ◽

Urszula Kurczewska ◽

Katarzyna Derwinska ◽

Wojciech Orlowski ◽

Daria Orszulak-Michalak

Keyword(s):

Immune Response ◽

Wound Dressings ◽

Enzyme Linked Immunosorbent Assay ◽

In Vitro Evaluation ◽

Murine Fibroblasts ◽

The Subject ◽

Immunogenic Properties

Abstract The aim of this study was in vitro evaluation of the level of the immune response in relation to wound dressings composed of alginate, calcium carboxymethylcellulose, and dibutyrylochitin and determination of the direction of response, which will make referring next to the results of in vivo phase possible. The subject of the experiments was to examine the commercially available, biodegradable alginate dressing, commercially available but not biodegradable dressing constructed from the sodium carboxymethylcellulose, and synthesized in house biodegradable dressing constructed of the dibutyrylchitin. To determine the direction of the immune response, the degree of secretion of pro-inflammatory interleukin (IL-1, IL-6) and antiinflammatory (IL-10) interleukin from murine fibroblasts having contact with the tested dressings (ELISA enzyme linked immunosorbent assay), was tested.

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