scholarly journals Therapeutic drug monitoring in Davydovsky Hospital: using HPLC–MS/MS method in practical healthcare

2020 ◽  
Vol 4 (35) ◽  
pp. 47-52
Author(s):  
T. A. Rodina ◽  
E. S. Melnikov
Keyword(s):  
Mass Spectrometry ◽  
Side Effects ◽  
Therapeutic Drug Monitoring ◽  
Drug Monitoring ◽  
Drug Dosage ◽  
Individual Therapy ◽  
Therapeutic Window ◽  
Therapeutic Drug ◽  
Tandem Mass ◽  
Anticoagulant Drugs

There was shown that therapeutic drug monitoring by liquid tandem mass spectrometry is essential not only for the drugs with a narrow therapeutic window but also for “simple” medicines like anti‑hypertension and anticoagulant drugs. The examples of choosing an individual therapy and adjusting the drug dosage to eliminate side effects were given. The cases of solving non‑standard situations that arise during bioanalytical studies were considered.

scholarly journals Multiplex Liquid Chromatography-Tandem Mass Spectrometry Assay for Simultaneous Therapeutic Drug Monitoring of Ribavirin, Boceprevir, and Telaprevir

2013 ◽  
Vol 57 (7) ◽  
pp. 3147-3158 ◽  
Author(s):  
Manel Aouri ◽  
Darius Moradpour ◽  
Matthias Cavassini ◽  
Thomas Mercier ◽  
Thierry Buclin ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Hepatitis C ◽  
Therapeutic Drug Monitoring ◽  
Tandem Mass Spectrometry ◽  
Antiviral Therapy ◽  
Drug Monitoring ◽  
Reversed Phase ◽  
Therapeutic Drug ◽  
Tandem Mass

ABSTRACTNew directly acting antivirals (DAAs) that inhibit hepatitis C virus (HCV) replication are increasingly used for the treatment of chronic hepatitis C. A marked pharmacokinetic variability and a high potential for drug-drug interactions between DAAs and numerous drug classes have been identified. In addition, ribavirin (RBV), commonly associated with hemolytic anemia, often requires dose adjustment, advocating for therapeutic drug monitoring (TDM) in patients under combined antiviral therapy. However, an assay for the simultaneous analysis of RBV and DAAs constitutes an analytical challenge because of the large differences in polarity among these drugs, ranging from hydrophilic (RBV) to highly lipophilic (telaprevir [TVR]). Moreover, TVR is characterized by erratic behavior on standard octadecyl-based reversed-phase column chromatography and must be separated from VRT-127394, its inactive C-21 epimer metabolite. We have developed a convenient assay employing simple plasma protein precipitation, followed by high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) for the simultaneous determination of levels of RBV, boceprevir, and TVR, as well as its metabolite VRT-127394, in plasma. This new, simple, rapid, and robust HPLC-MS/MS assay offers an efficient method of real-time TDM aimed at maximizing efficacy while minimizing the toxicity of antiviral therapy.

Therapeutic Drug Monitoring of Tacrolimus (FK506) Using Tandem Mass Spectrometry

1998 ◽  
Vol 20 (2) ◽  
pp. 240-241 ◽  
Author(s):  
Paul J. Taylor ◽  
Stephen V. Lynch ◽  
Glenda A. Balderson ◽  
Anthony G. Johnson
Keyword(s):  
Mass Spectrometry ◽  
Therapeutic Drug Monitoring ◽  
Tandem Mass Spectrometry ◽  
Drug Monitoring ◽  
Therapeutic Drug ◽  
Tandem Mass

scholarly journals Simultaneous Quantification of Methotrexate and Its Metabolite 7-Hydroxy-Methotrexate in Human Plasma for Therapeutic Drug Monitoring

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Xinxin Ren ◽  
Zhipeng Wang ◽  
Yunlei Yun ◽  
Guangyi Meng ◽  
Xialan Zhang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Therapeutic Drug Monitoring ◽  
Human Plasma ◽  
Drug Monitoring ◽  
Internal Standard ◽  
Gradient Elution ◽  
Therapeutic Drug ◽  
Tandem Mass ◽  
The Matrix ◽  
Analytical Time

Objective. To establish and validate a simple, sensitive, and rapid liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the determination of methotrexate (MTX) and its major metabolite 7-hydroxy-methotrexate (7-OH-MTX) in human plasma. Method. The chromatographic separation was achieved on a Zorbax C18 column (3.5 μm, 2.1 × 100 mm) using a gradient elution with methanol (phase B) and 0.2% formic acid aqueous solution (phase A). The flow rate was 0.3 mL/min with analytical time of 3.5 min. Mass spectrometry detection was performed in a triple-quadruple tandem mass spectrometer under positive ion mode with the following mass transitions: m/z 455.1/308.1 for MTX, 471.0/324.1 for 7-OH-MTX, and 458.2/311.1 for internal standard. The pretreatment procedure was optimized with dilution after one-step protein precipitation. Results. The calibration range of methotrexate and 7-OH-MTX was 5.0-10000.0 ng/mL. The intraday and interday precision and accuracy were less than 15% and within ±15% for both analytes. The recovery for MTX and 7-OH-MTX was more than 90% and the matrix effect ranged from 97.90% to 117.60%. Conclusion. The method was successfully developed and applied to the routine therapeutic drug monitoring of MTX and 7-OH-MTX in human plasma.

scholarly journals Improved Therapeutic Drug Monitoring of Tacrolimus (FK506) by Tandem Mass Spectrometry

1997 ◽  
Vol 43 (11) ◽  
pp. 2189-2190 ◽  
Author(s):  
Paul J Taylor ◽  
Nicholas S Hogan ◽  
Stephen V Lynch ◽  
Anthony G Johnson ◽  
Susan M Pond
Keyword(s):  
Mass Spectrometry ◽  
Therapeutic Drug Monitoring ◽  
Tandem Mass Spectrometry ◽  
Drug Monitoring ◽  
Therapeutic Drug ◽  
Tandem Mass
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