scholarly journals PENGARUH LISAT PLATELET FASE SIKLUS MENSTRUASI TERHADAP AKTIVITAS FIBROBLAS KULIT MANUSIA

2019 ◽  
Vol 46 (1) ◽  
Author(s):  
Thianti Sylviningrum ◽  
Flandiana Yogianti ◽  
Sunardi Radiono ◽  
Yohanes Widodo Wirohadidjojo
Keyword(s):  
Growth Factors ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Platelet Rich Fibrin ◽  
Fetal Bovine

Lisat platelet dari platelet rich fibrin (PRF) adalah material berisi growth factors, estradiol (E2), dan leptin. Pada fase ovulasi (FO) dijumpai kadar E2, jumlah platelet, dan leukosit tertinggi, dimana interaksinya dapat menghasilkan growth factors yang meningkatkan aktivitas fibroblas dermis. Penelitian ini bertujuan untuk mengetahui efek lisat platelet dari PRF fase bleeding (FB) dan FO pada aktivitas fibroblas dermis.Biakan fibroblas dermis dibedakan menjadi 3 kelompok, biakan dalam media kultur dan fetal bovine serum (FBS) 1% sebagai kontrol negatif, biakan dalam media kultur dan FBS 10% sebagai kontrol positif. Kelompok perlakuan adalah biakan fibroblas yang diberi lisat platelet dari PRF FB dan FO 10%, 20%, dan 40% selama 72 jam.Perbedaan bermakna (p<0,05) dijumpai pada rerata indeks proliferasi antara kelompok kontrol positif dengan fibroblas terpajan lisat platelet dari PRF FB dan FO 20% dibandingkan dengan kontrol negatif, serta rerata densitas optik (DO) timbunan kolagen dari fibroblas yang diberi lisat platelet dari PRF FB dan FO 20% dibandingkan dengan semua kelompok kontrol. Tidak ada perbedaan bermakna (p>0,05) pada rerata indeks proliferasi dan DO timbunan kolagen antara kelompok fibroblas yang terpajan semua konsentrasi lisat platelet dari PRF FB dan FO. Peningkatan aktivitas fibroblas dermis oleh lisat platelet dari PRF FO tidak berbeda dibandingkan dengan lisat platelet dari PRF FB.Kata kunci : lisat platelet, platelet rich fibrin, fase bleeding, fase ovulasi, fibroblas, kolagen

scholarly journals Identification and Quantification of Proteins from Preparative Partition Chromatography and Peptides from Organic Extraction of Fetal Versus Adult Bovine Serum using Nano-Spray-LC-ESI-MS/MS

2021 ◽  
Author(s):  
Zhuo Zhen Chen
Keyword(s):  
Growth Factors ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Intact Proteins ◽  
Endogenous Peptides ◽  
Fetal Serum ◽  
Adult Bovine Serum ◽  
Alpha Feto Protein ◽  
Fetal Bovine ◽  
Insulin Like Growth Factors

Blood proteins communicate with many different cells, tissue and organs; perform key functions in the immune system and may be of particular biological complexity. One of the most widely used blood products in the laboratory is fetal bovine serum for cell culture. There are ethical and practical concerns regarding the use of fetal serum from animals and alternative serum-free replacements have been attempted using platelet lysates. Previous biochemical experiments have shown that FBS apparently contained factors such as alpha-feto protein (AFP) and insulin-like growth factors that may support the indefinite cell growth and division of certain cell lines. It is presumed that a set of as yet undefined growth factors transform cells growth resulting in rapid proliferation. Cultured Raw cells 264.7 in adult bovine serum multiplied slowly and differentiated into elongated cells with a dendritic shape, which died after the first few generations. On the contrary, in fetal bovine serum, cultured cells multiplied rapidly and formed many smaller cells with a rounded shape through many cell passages. Three independent batches of fetal bovine serum were tested on Raw cells 264.7 macrophages to confirm that they supported cell growth in culture compared to three independent batches of adult bovine serum. The intact proteins of each serum sample were separated by partition chromatography into 16 fractions with an increasing step gradient of salts over quaternary amine resin (proteomics). The endogenous peptides were precipitated with 90% of acetonitrile and extracted into 10 fractions with a decreasing step gradient of acetonitrile in water (peptidomics). Trypsin digested intact proteins and endogenous peptides were then analyzed on a fresh C18 nano-HPLC column with random and independent sampling by LC-ESI-MS/MS. The fractionated mass spectra were identified with SEQUEST and X!TANDEM algorithms. Redundant use of MS/MS spectra were flirted out with the SQL Server system and the R statistical analysis system was used to perform Chi Square (X2) analysis of frequency counts and ANOVA of the log10 precursor intensity results. Alpha-feto protein, fetal albumin, insulin, insulin like growth factors, platelet derived growth factors and proteins associated with HRAS/AKT growth pathway at the level of ligand, receptors, receptor associated enzyme and nucleic acid binding proteins including transcription factors were observed to be specifically enriched in fetal serum.

scholarly journals Platelet-Rich Fibrin Extract: A Promising Fetal Bovine Serum Alternative in Explant Cultures of Human Periosteal Sheets for Regenerative Therapy

2019 ◽  
Vol 20 (5) ◽  
pp. 1053 ◽  
Author(s):  
Tomoyuki Kawase ◽  
Masaki Nagata ◽  
Kazuhiro Okuda ◽  
Takashi Ushiki ◽  
Yoko Fujimoto ◽  
...  
Keyword(s):  
Bovine Serum ◽  
Culture Medium ◽  
Fetal Bovine Serum ◽  
Antigen Expression ◽  
Explant Culture ◽  
The Novel ◽  
Regenerative Therapy ◽  
Platelet Rich Fibrin ◽  
Alp Activity ◽  
Fetal Bovine

In 2004, we developed autologous periosteal sheets for the treatment of periodontal bone defects. This regenerative therapy has successfully regenerated periodontal bone and augmented alveolar ridge for implant placement. However, the necessity for 6-week culture is a limitation. Here, we examined the applicability of a human platelet-rich fibrin extract (PRFext) as an alternative to fetal bovine serum (FBS) for the explant culture of periosteal sheets in a novel culture medium (MSC-PCM) originally developed for maintaining mesenchymal stem cells. Small periosteum tissue segments were expanded in MSC-PCM + 2% PRFext for 4 weeks, and the resulting periosteal sheets were compared with those prepared by the conventional method using Medium199 + 10% FBS for their growth rate, cell multilayer formation, alkaline phosphatase (ALP) activity, and surface antigen expression (CD73, CD90, and CD105). Periosteal sheets grew faster in the novel culture medium than in the conventional medium. However, assessment of cell shape and ALP activity revealed that the periosteal cells growing in the novel medium were relatively immature. These findings suggest that the novel culture medium featuring PRFext offers advantages by shortening the culture period and excluding possible risks associated with xeno-factors without negatively altering the activity of periosteal sheets.

Clinical grade cultivation of human Schwann cell, by the using of human autologous serum instead of fetal bovine serum and without growth factors

2011 ◽  
Vol 13 (2) ◽  
pp. 281-285 ◽  
Author(s):  
Hamid-Reza Aghayan ◽  
Babak Arjmand ◽  
Abbas Norouzi-Javidan ◽  
Hooshang Saberi ◽  
Masoud Soleimani ◽  
...  
Keyword(s):  
Growth Factors ◽  
Schwann Cell ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Autologous Serum ◽  
Clinical Grade ◽  
Fetal Bovine

scholarly journals Comparison of Human Platelet Lysate versus Fetal Bovine Serum for Expansion of Human Articular Cartilage–Derived Chondroprogenitors

Cartilage ◽  
2020 ◽  
pp. 194760352091863 ◽  
Author(s):  
Upasana Kachroo ◽  
Shikha Mary Zachariah ◽  
Augustine Thambaiah ◽  
Aleya Tabasum ◽  
Abel Livingston ◽  
...  
Keyword(s):  
Growth Factors ◽  
Bovine Serum ◽  
Human Platelet ◽  
Fetal Bovine Serum ◽  
Surface Marker ◽  
Platelet Lysate ◽  
Surface Marker Expression ◽  
Trilineage Differentiation ◽  
Human Platelet Lysate ◽  
Fetal Bovine

Purpose Articular chondroprogenitors, a suitable contender for cell-based therapy in cartilage repair, routinely employ fetal bovine serum (FBS) for expansion and differentiation. The possibility of transplant rejections or zoonoses transmissions raise a need for xeno-free alternatives. Use of human platelet lysate (hPL), a nutrient supplement abundant in growth factors, has not been reported for human chondroprogenitor expansion thus far. Our aim was to compare the biological profile of chondroprogenitors grown in hPL versus FBS. Methods Chondroprogenitors were isolated from 3 osteoarthritic knee joints. Following differential fibronectin adhesion assay, passage 0 cells grown in (a) 10% FBS and (b) 10% hPL were considered for assessment of growth kinetics, surface marker expression, gene expression, and trilineage differentiation. Latent transforming growth factor–β1 (TGFβ1) levels were also measured for each culture medium used. Results Cellular proliferation was significantly higher in cells grown with hPL ( P < 0.01). Surface marker expression was comparable except in CD-146 where hPL group had significantly higher values ( P = 0.03). Comparison of mRNA expression revealed notably low values of collagen I, collagen X, aggrecan, and collagen II ( P < 0.05). Trilineage differentiation was seen in both groups with higher alizarin red uptake noted in hPL. There were also significantly higher levels of latent TGFβ1 in the medium containing hPL as compared to FBS. Conclusions This is the first in vitro xeno-free study to affirm that hPL can serve as an optimal growth supplement for expansion of articular chondroprogenitors, although an in-depth assessment of resident growth factors and evaluation of different dilutions of hPL is required to assess suitability for use in translational research.

scholarly journals Identification and Quantification of Proteins from Preparative Partition Chromatography and Peptides from Organic Extraction of Fetal Versus Adult Bovine Serum using Nano-Spray-LC-ESI-MS/MS

2021 ◽  
Author(s):  
Zhuo Zhen Chen
Keyword(s):  
Growth Factors ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Intact Proteins ◽  
Endogenous Peptides ◽  
Fetal Serum ◽  
Adult Bovine Serum ◽  
Alpha Feto Protein ◽  
Fetal Bovine ◽  
Insulin Like Growth Factors

Blood proteins communicate with many different cells, tissue and organs; perform key functions in the immune system and may be of particular biological complexity. One of the most widely used blood products in the laboratory is fetal bovine serum for cell culture. There are ethical and practical concerns regarding the use of fetal serum from animals and alternative serum-free replacements have been attempted using platelet lysates. Previous biochemical experiments have shown that FBS apparently contained factors such as alpha-feto protein (AFP) and insulin-like growth factors that may support the indefinite cell growth and division of certain cell lines. It is presumed that a set of as yet undefined growth factors transform cells growth resulting in rapid proliferation. Cultured Raw cells 264.7 in adult bovine serum multiplied slowly and differentiated into elongated cells with a dendritic shape, which died after the first few generations. On the contrary, in fetal bovine serum, cultured cells multiplied rapidly and formed many smaller cells with a rounded shape through many cell passages. Three independent batches of fetal bovine serum were tested on Raw cells 264.7 macrophages to confirm that they supported cell growth in culture compared to three independent batches of adult bovine serum. The intact proteins of each serum sample were separated by partition chromatography into 16 fractions with an increasing step gradient of salts over quaternary amine resin (proteomics). The endogenous peptides were precipitated with 90% of acetonitrile and extracted into 10 fractions with a decreasing step gradient of acetonitrile in water (peptidomics). Trypsin digested intact proteins and endogenous peptides were then analyzed on a fresh C18 nano-HPLC column with random and independent sampling by LC-ESI-MS/MS. The fractionated mass spectra were identified with SEQUEST and X!TANDEM algorithms. Redundant use of MS/MS spectra were flirted out with the SQL Server system and the R statistical analysis system was used to perform Chi Square (X2) analysis of frequency counts and ANOVA of the log10 precursor intensity results. Alpha-feto protein, fetal albumin, insulin, insulin like growth factors, platelet derived growth factors and proteins associated with HRAS/AKT growth pathway at the level of ligand, receptors, receptor associated enzyme and nucleic acid binding proteins including transcription factors were observed to be specifically enriched in fetal serum.

Treatment of fetal bovine serum improves early development of porcine embryos by alleviating oxidative stress

2014 ◽  
Author(s):  
Seon-A Choi ◽  
Seong-Eun Mun ◽  
Pil-Soo Jeong ◽  
Hae-Jun Yang ◽  
Seung-Bin Yoon ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Early Development ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Fetal Bovine
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