scholarly journals Fluorescent AIE-Active Materials for Two-Photon Bioimaging Applications

2020 ◽  
Vol 8 ◽  
Author(s):  
Qing Lu ◽  
Cheng-Juan Wu ◽  
Zhiqiang Liu ◽  
Guangle Niu ◽  
Xiaoqiang Yu
Keyword(s):  
Fluorescence Imaging ◽  
Ex Vivo ◽  
Tissue Imaging ◽  
Active Materials ◽  
Two Photon ◽  
Real Time Visualization ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Fluorescence imaging has been widely used as a powerful tool for in situ and real-time visualization of important analytes and biological events in live samples with remarkably high selectivity, sensitivity, and spatial resolution. Compared with one-photon fluorescence imaging, two-photon fluorescence imaging exhibits predominant advantages of minimal photodamage to samples, deep tissue penetration, and outstanding resolution. Recently, the aggregation-induced emission (AIE) materials have become a preferred choice in two-photon fluorescence biological imaging because of its unique bright fluorescence in solid and aggregate states and strong resistance to photobleaching. In this review, we will exclusively summarize the applications of AIE-active materials in two-photon fluorescence imaging with some representative examples from four aspects: fluorescence detection, in vitro cell imaging, ex vivo tissue imaging, and in vivo vascular imaging. In addition, the current challenges and future development directions of AIE-active materials for two-photon bioimaging are briefly discussed.

A series of Zn(ii) terpyridine complexes with enhanced two-photon-excited fluorescence for in vitro and in vivo bioimaging

2015 ◽  
Vol 3 (36) ◽  
pp. 7213-7221 ◽  
Author(s):  
Qiong Zhang ◽  
Xiaohe Tian ◽  
Zhangjun Hu ◽  
Caroline Brommesson ◽  
Jieying Wu ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Cross Sections ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

TPA cross sections are enhanced for the complexes containing D–A type ligandL1.1exhibits specificity in two-photon fluorescence imaging.

Silole-Based Red Fluorescent Organic Dots for Bright Two-Photon Fluorescence In vitro Cell and In vivo Blood Vessel Imaging

Small ◽  
2015 ◽  
Vol 12 (6) ◽  
pp. 782-792 ◽  
Author(s):  
Bin Chen ◽  
Guangxue Feng ◽  
Bairong He ◽  
Chiching Goh ◽  
Shidang Xu ◽  
...  
Keyword(s):  
Blood Vessel ◽  
Two Photon ◽  
Vessel Imaging ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

scholarly journals Two-Photon Microscopy Allows Imaging and Characterization of Cochlear Microvasculature In Vivo

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Friedrich Ihler ◽  
Mattis Bertlich ◽  
Bernhard Weiss ◽  
Steffen Dietzel ◽  
Martin Canis
Keyword(s):  
Fluorescence Microscopy ◽  
Inner Ear ◽  
Ex Vivo ◽  
Small Scale ◽  
Published Data ◽  
Cochlear Blood Flow ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Impairment of cochlear blood flow has been discussed as factor in the pathophysiology of various inner ear disorders. However, the microscopic study of cochlear microcirculation is limited due to small scale and anatomical constraints. Here, two-photon fluorescence microscopy is applied to visualize cochlear microvessels. Guinea pigs were injected with Fluorescein isothiocyanate- or Texas red-dextrane as plasma marker. Intravital microscopy was performed in four animals and explanted cochleae from four animals were studied. The vascular architecture of the cochlea was visualized up to a depth of90.0±22.7 μm. Imaging yielded a mean contrast-to-noise ratio (CNR) of3.3±1.7. Mean diameter in vivo was16.5±6.0 μm for arterioles and8.0±2.4 μm for capillaries. In explanted cochleae, the diameter of radiating arterioles and capillaries was measured with12.2±1.6 μm and6.6±1.0 μm, respectively. The difference between capillaries and arterioles was statistically significant in both experimental setups (P<0.001andP=0.022, two-way ANOVA). Measured vessel diameters in vivo and ex vivo were in agreement with published data. We conclude that two-photon fluorescence microscopy allows the investigation of cochlear microvessels and is potentially a valuable tool for inner ear research.

Unprecedentedly High Tissue Penetration Capability of Co-Assembled Nanosystems for Two-Photon Fluorescence Imaging In Vivo

2015 ◽  
Vol 3 (5) ◽  
pp. 646-651 ◽  
Author(s):  
Pei-Pei Yang ◽  
Yang Yang ◽  
Yu-Juan Gao ◽  
Yi Wang ◽  
Ju-Chen Zhang ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Tissue Penetration ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Color‐Tunable Light‐up Bioorthogonal Probes for In Vivo Two‐Photon Fluorescence Imaging

2020 ◽  
Vol 26 (20) ◽  
pp. 4576-4582 ◽  
Author(s):  
Yandong Dou ◽  
Yajun Wang ◽  
Yukun Duan ◽  
Bin Liu ◽  
Qinglian Hu ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Two Photon ◽  
Color Tunable ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Water-soluble small-molecule probes for RNA based on a two-photon fluorescence “off–on” process: systematic analysis in live cell imaging and understanding of structure–activity relationships

2017 ◽  
Vol 53 (99) ◽  
pp. 13245-13248 ◽  
Author(s):  
Hong Li ◽  
Yuncang Li ◽  
Huihui Zhang ◽  
Guoyong Xu ◽  
Yuliang Zhang ◽  
...  
Keyword(s):  
Water Soluble ◽  
Systematic Analysis ◽  
Pyridinium Cation ◽  
Two Photon ◽  
Small Molecule Probes ◽  
Structure Activity ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

The remarkable two-photon fluorescence responses in vitro and in vivo of L1–5 for RNA revealed the synergistic effect of the amino group and the pyridinium cation.

Simultaneous atomic-force and two-photon fluorescence imaging of biological specimens in vivo

2004 ◽  
Vol 99 (4) ◽  
pp. 235-245 ◽  
Author(s):  
Claudiu C. Gradinaru ◽  
Peter Martinsson ◽  
Thijs J. Aartsma ◽  
Thomas Schmidt
Keyword(s):  
Fluorescence Imaging ◽  
Two Photon ◽  
Atomic Force ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

scholarly journals Longitudinal in vivo two-photon fluorescence imaging

2014 ◽  
Vol 522 (8) ◽  
pp. 1708-1727 ◽  
Author(s):  
Sarah E. Crowe ◽  
Graham C.R. Ellis-Davies
Keyword(s):  
Fluorescence Imaging ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence
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