Water-soluble small-molecule probes for RNA based on a two-photon fluorescence “off–on” process: systematic analysis in live cell imaging and understanding of structure–activity relationships

2017 ◽  
Vol 53 (99) ◽  
pp. 13245-13248 ◽  
Author(s):  
Hong Li ◽  
Yuncang Li ◽  
Huihui Zhang ◽  
Guoyong Xu ◽  
Yuliang Zhang ◽  
...  
Keyword(s):  
Water Soluble ◽  
Systematic Analysis ◽  
Pyridinium Cation ◽  
Two Photon ◽  
Small Molecule Probes ◽  
Structure Activity ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

The remarkable two-photon fluorescence responses in vitro and in vivo of L1–5 for RNA revealed the synergistic effect of the amino group and the pyridinium cation.

Silole-Based Red Fluorescent Organic Dots for Bright Two-Photon Fluorescence In vitro Cell and In vivo Blood Vessel Imaging

Small ◽  
2015 ◽  
Vol 12 (6) ◽  
pp. 782-792 ◽  
Author(s):  
Bin Chen ◽  
Guangxue Feng ◽  
Bairong He ◽  
Chiching Goh ◽  
Shidang Xu ◽  
...  
Keyword(s):  
Blood Vessel ◽  
Two Photon ◽  
Vessel Imaging ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

A series of Zn(ii) terpyridine complexes with enhanced two-photon-excited fluorescence for in vitro and in vivo bioimaging

2015 ◽  
Vol 3 (36) ◽  
pp. 7213-7221 ◽  
Author(s):  
Qiong Zhang ◽  
Xiaohe Tian ◽  
Zhangjun Hu ◽  
Caroline Brommesson ◽  
Jieying Wu ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Cross Sections ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

TPA cross sections are enhanced for the complexes containing D–A type ligandL1.1exhibits specificity in two-photon fluorescence imaging.

scholarly journals In situ imaging of aminopeptidase N activity in hepatocellular carcinoma: a migration model for tumour using an activatable two-photon NIR fluorescent probe

2019 ◽  
Vol 10 (6) ◽  
pp. 1619-1625 ◽  
Author(s):  
Haidong Li ◽  
Yueqing Li ◽  
Qichao Yao ◽  
Jiangli Fan ◽  
Wen Sun ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Fluorescent Probe ◽  
Fluorescence Probe ◽  
Aminopeptidase N ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

In this work, a novel NIR two-photon fluorescence probe DCM-APN is reported for tracking APN in vitro and in vivo.

scholarly journals Fluorescent AIE-Active Materials for Two-Photon Bioimaging Applications

2020 ◽  
Vol 8 ◽  
Author(s):  
Qing Lu ◽  
Cheng-Juan Wu ◽  
Zhiqiang Liu ◽  
Guangle Niu ◽  
Xiaoqiang Yu
Keyword(s):  
Fluorescence Imaging ◽  
Ex Vivo ◽  
Tissue Imaging ◽  
Active Materials ◽  
Two Photon ◽  
Real Time Visualization ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Fluorescence imaging has been widely used as a powerful tool for in situ and real-time visualization of important analytes and biological events in live samples with remarkably high selectivity, sensitivity, and spatial resolution. Compared with one-photon fluorescence imaging, two-photon fluorescence imaging exhibits predominant advantages of minimal photodamage to samples, deep tissue penetration, and outstanding resolution. Recently, the aggregation-induced emission (AIE) materials have become a preferred choice in two-photon fluorescence biological imaging because of its unique bright fluorescence in solid and aggregate states and strong resistance to photobleaching. In this review, we will exclusively summarize the applications of AIE-active materials in two-photon fluorescence imaging with some representative examples from four aspects: fluorescence detection, in vitro cell imaging, ex vivo tissue imaging, and in vivo vascular imaging. In addition, the current challenges and future development directions of AIE-active materials for two-photon bioimaging are briefly discussed.

A novel two-photon fluorescent probe for hydrogen sulfide in living cells using an acedan–NBD amine dyad based on FRET process with high selectivity and sensitivity

2017 ◽  
Vol 41 (14) ◽  
pp. 6769-6774 ◽  
Author(s):  
Yao Tang ◽  
Guo-Fang Jiang
Keyword(s):  
Hydrogen Sulfide ◽  
Linear Response ◽  
High Selectivity ◽  
Fluorescence Probe ◽  
Living Cells ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

The first NBD amine based two-photon fluorescence probe L using a FRET strategy was developed for the H2S detecting in vitro and in vivo. The probe L not only afforded high selectivity and sensitivity for H2S detecting, but also dispalyed a linear response to H2S with a low detection limit 24 nM.

A water-soluble two-photon fluorescence chemosensor for ratiometric imaging of mitochondrial viscosity in living cells

2016 ◽  
Vol 4 (35) ◽  
pp. 5907-5912 ◽  
Author(s):  
Meng Zhao ◽  
Yingzhong Zhu ◽  
Jian Su ◽  
Qian Geng ◽  
Xiaohe Tian ◽  
...  
Keyword(s):  
Living Cells ◽  
Water Soluble ◽  
Two Photon ◽  
Ratiometric Imaging ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

We report a novel water-soluble ratiometric TPEF chemosensor EIN that is specifically responsive and singularly sensitive to mitochondria viscosity in living cells.

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