Novel Quantitative Methods for the Determination of Biomaterial Cytotoxicity

Two novel methods for the determination of biomaterial cytotoxicity using cell culture are presented. The methods combine a standardized protocol for producing extracts from medical devices with either the established MTT assay or a new fluorimetric assay. The suitability of both methods for evaluating the toxicity of candidate materials was demonstrated by resolution of the differences in the toxic effects of serial dilutions of a PVC extract on BHK21 and HT1080 cells. The tests yield highly reproducible, quantitative results and can be applied to materials in the usual physical forms applicable to artificial organs.

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An Improved Quantitative Image Analyser

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100078663 ◽

1977 ◽

Vol 35 ◽

pp. 226-227

Author(s):

J.P. Fallon ◽

P.J. Gregory ◽

C.J. Taylor

Keyword(s):

Feature Extraction ◽

Quantitative Analysis ◽

Frequency Content ◽

General Sense ◽

Physical Measurements ◽

Quantitative Image ◽

Image Analyser ◽

Automatic Methods ◽

Quantitative Results

Quantitative image analysis systems have been used for several years in research and quality control applications in various fields including metallurgy and medicine. The technique has been applied as an extension of subjective microscopy to problems requiring quantitative results and which are amenable to automatic methods of interpretation.Feature extraction. In the most general sense, a feature can be defined as a portion of the image which differs in some consistent way from the background. A feature may be characterized by the density difference between itself and the background, by an edge gradient, or by the spatial frequency content (texture) within its boundaries. The task of feature extraction includes recognition of features and encoding of the associated information for quantitative analysis.Quantitative Analysis. Quantitative analysis is the determination of one or more physical measurements of each feature. These measurements may be straightforward ones such as area, length, or perimeter, or more complex stereological measurements such as convex perimeter or Feret's diameter.

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Determination of anti-oxidative effects of phytogenic substances using a porcine epithelial cell culture model

Planta Medica ◽

10.1055/s-0036-1596997 ◽

2016 ◽

Vol 81 (S 01) ◽

pp. S1-S381

Author(s):

T Kaschubek ◽

E Mayer ◽

G Schatzmayr ◽

K Teichmann

Keyword(s):

Cell Culture ◽

Epithelial Cell ◽

Cell Culture Model ◽

Epithelial Cell Culture ◽

Culture Model ◽

Oxidative Effects

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Method for the determination of the radio-opacity index of medical devices made of plastics materials

10.3403/00094046u ◽

2015 ◽

Keyword(s):

Medical Devices

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Determination of Specific Activity of Cholera Chemical Vaccine Components using Cell Culture

Biotekhnologiya ◽

10.21519/0234-2758-2020-36-3-82-89 ◽

2020 ◽

Vol 36 (3) ◽

pp. 82-89

Author(s):

O.V. Gromova ◽

O.S. Durakova ◽

S.V. Generalov ◽

L.F. Livanova ◽

O.A. Volokh

Keyword(s):

Cell Culture ◽

Vibrio Cholerae ◽

Production Control ◽

Specific Activity ◽

Methodological Approach ◽

Toxin Production ◽

Submerged Cultivation ◽

Vaccine Production ◽

Antigenic Activity

Том 36(2020) №3 стр. 82-89; DOI 10.21519/0234-2758-2020-36-3-82-89А.В. Гаева1*, О.В. Громова1, О.С. Дуракова1, С.В. Генералов1, Л.Ф. Ливанова1, О.А. Волох1 Определение специфической активности компонентов холерной химической вакцины с использованием культуры клеток 1ФКУЗ «Российский научно-исследовательский противочумный институт «Микроб»» Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека, Саратов 410005 *[email protected] Поступила - 2019-11-26; После доработки - 2020-03-16; Принята к публикации - 2020-05-15 Список литературы Описаны методы определения динамики продукции токсинов штаммом Vibrio cholerae 569B при глубинном культивировании в биореакторе и антигенной активности специфической фракции холерогена-анатоксина по анатоксинсвязыванию с использованием клеточных культур. Показана высокая степень соответствия результатов, полученных методами, применяемыми для контроля этапов производства холерной химической вакцины и рассмотренными в данной работе. Отмечено, что применение клеточной линии СНО-К1 наиболее перспективно для замены биомоделей на промежуточных этапах контроля активных компонентов холерной химической вакцины. Разработанный методический подход впервые предлагается использовать на этапах производства холерной бивалентной химической вакцины. культура клеток, Vibrio cholerae, холерная химическая вакцина, контроль производства, холера. Vol 36(2020) N 3 p. 82-89; DOI 10.21519/0234-2758-2020-36-3-82-89A.V. Gaeva1*, O.V. Gromova1, O.S. Durakova1, S.V. Generalov1, L.F. Livanova1, O.A. Volokh1 Determination of Specific Activity of Cholera Chemical Vaccine Components using Cell Culture 1Russian Research Anti-Plague Institute «Microbe» of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Saratov, 410005 *[email protected] Received - 26.11.2019; Accepted - 15.05.2020 References The methods has been described to determine the dynamics of toxin production by the Vibrio cholerae 569B strain during submerged cultivation in bioreactor and of the antigenic activity of specific choleragen anatoxin fraction by anatoxin binding levels using cell cultures. High degree of consistency was observed between the results obtained via the method under consideration and those obtained via control methods at different stages of cholera chemical vaccine production. It was shown that the CHO-K1 cell line is the most promising substitute for biomodels at the intermediate stages of control of active cholera chemical vaccine components. The developed methodological approach was first proposed for use at the stages of cholera chemical bivalent vaccine manufacturing. cell culture, Vibrio cholerae, cholera chemical vaccine, production control, cholera.

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