scholarly journals Transposon insertion mapping with PIMMS – Pragmatic Insertional Mutation Mapping System

2015 ◽  
Vol 06 ◽  
Author(s):  
Adam M. Blanchard ◽  
James A. Leigh ◽  
Sharon A. Egan ◽  
Richard D. Emes
2018 ◽  
Vol 50 (04) ◽  
pp. 541-546
Author(s):  
R. H. SHAH ◽  
S. MEMON ◽  
I. A. ISMAILI ◽  
Z. HUSSAIN ◽  
M. HAMMAD ◽  
...  

Genetics ◽  
2001 ◽  
Vol 158 (4) ◽  
pp. 1825-1827 ◽  
Author(s):  
Heather A Wiatrowski ◽  
Marian Carlson

Abstract We describe a new approach for identifying the gene corresponding to a mutation in Saccharomyces cerevisiae. A library of mTn-lacZ/LEU2 insertions is tested for failure to complement the mutation, and the noncomplementing insertion is used to obtain sequence. This approach offers an alternative to cloning by complementation with a plasmid library.


2004 ◽  
Vol 52 (Suppl 2) ◽  
pp. S357.3-S357
Author(s):  
D. Xing ◽  
F. G. Devecchi ◽  
T. R. Staley ◽  
D. S. Glassman ◽  
J. B. Martins

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