scholarly journals Dim Red Light During Scotophase Enhances Mating of a Moth Through Increased Male Antennal Sensitivity Against the Female Sex Pheromone

2021 ◽  
Vol 12 ◽  
Author(s):  
Qiuying Chen ◽  
Xi Yang ◽  
Dongrui You ◽  
Jiaojiao Luo ◽  
Xiaojing Hu ◽  
...  

Insects are behaviorally and physiologically affected by different light conditions, including photoperiod, light intensity, and spectrum. Light at night has important influences on nocturnal insects, including most moth species. Moth copulation and mating usually occur at night. Although a few studies examine changes in insect mating under artificial light at night, detailed influences of light, such as that of monochromatic light, on moth mating remain largely unknown. In this study, on the basis of long-term insects rearing experience, dim red light (spectrum range: 610–710nm, with a peak at 660nm; 2.0 Lux) during scotophase was hypothesized to enhance mating in the yellow peach moth, Conogethes punctiferalis. To test the hypothesis, the mating of moths under dim red, blue, and white lights during scotophase was observed. Under the dim red light, the enhancement of mating in C. punctiferalis was observed. In addition, the electroantennografic response of males against the female sex pheromone increased with red light treatment during scotophase. In an analysis of the differentially expressed genes in the antennae of males under red light and dark conditions, the expression levels of two odorant-binding protein (OBP) genes, CpunOBP2 and CpunPBP5, were up-regulated. Two genes were then expressed in Escherichia coli, and the recombinant proteins showed strong binding to female pheromone components in fluorescence-binding assays. Thus, the results of this study indicated that dim red light at night enhanced the mating of C. punctiferalis. One of the mechanisms for the enhancement was probably an increase in the antennal sensitivity of males to the female sex pheromone under red light that was caused by increases in the expression levels of pheromone-binding protein genes in male antennae.

Author(s):  
Roland N. Perry

Abstract This chapter focuses on some of the principal techniques for analysing nematode behaviour and physiology, such as attraction/repulsion plate assays, pluronic gel assays, movement assays, electrophysiology, stylet activity, water content changes, oxygen consumption assays, collection of female sex pheromone, virgin females and males and viability tests, among others.


2011 ◽  
Vol 37 (11) ◽  
pp. 1216-1221 ◽  
Author(s):  
Chang Yeol Yang ◽  
Chae Hoon Paik ◽  
Geon Hwi Lee ◽  
Jin Young Park

Insects ◽  
2014 ◽  
Vol 5 (4) ◽  
pp. 705-721 ◽  
Author(s):  
Claire Duménil ◽  
Gary Judd ◽  
Dolors Bosch ◽  
Mario Baldessari ◽  
César Gemeno ◽  
...  

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