scholarly journals Differential Regulation of Damage-Associated Molecular Pattern Release in a Mouse Model of Skeletal Muscle Ischemia/Reperfusion Injury

2021 ◽  
Vol 12 ◽  
Author(s):  
Hiroaki Furubeppu ◽  
Takashi Ito ◽  
Midori Kakuuchi ◽  
Tomotsugu Yasuda ◽  
Chinatsu Kamikokuryo ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Gastrocnemius Muscle ◽  
Ischemia Reperfusion ◽  
Histone H3 ◽  
Organ Injury ◽  
Hindlimb Ischemia ◽  
Clinical Issue ◽  
Muscle Ischemia ◽  
Remote Organ ◽  
Remote Organ Injury

BackgroundSkeletal muscle ischemia/reperfusion (I/R) injury is an important clinical issue that can cause remote organ injury. Although its pathogenesis has not been fully elucidated, recent studies have suggested that damage-associated molecular patterns (DAMPs) are mediators of remote organ injury in sterile inflammation. The purpose of this study was to investigate the possible involvement of DAMPs, including the nuclear proteins high-mobility group box 1 (HMGB1) and histone H3, in the pathogenesis of skeletal muscle I/R injury in mice.MethodsHindlimb ischemia was induced in mice through bilateral ligation of inguinal regions using rubber grommets. Reperfusion was induced by cutting the rubber grommets after 2–12 h of ischemic period. Survival rates, localization of HMGB1 and histone H3 in the gastrocnemius muscle, and circulating HMGB1 and histone H3 levels were analyzed. The effect of anti-HMGB1 and anti-histone H3 antibodies on survival was analyzed in mice with I/R injury.ResultsAll mice with hindlimb ischemia survived for at least 36 h, while all mice died within 24 h if the hindlimbs were reperfused after ischemia for 4–12 h. Immunohistochemical analysis revealed that HMGB1 translocated from the nucleus to the cytoplasm in the ischemic gastrocnemius muscle, while histone H3 was confined to the nucleus. Accordingly, serum HMGB1 levels were significantly elevated in mice with hindlimb I/R compared with normal mice or mice with hindlimb ischemia (P < 0.05). Serum histone H3 levels were not elevated after I/R. Treatment with anti-HMGB1 antibodies significantly improved survival of mice with hindlimb I/R injury compared with control antibodies (P < 0.05).ConclusionsHMGB1, but not histone H3, translocated to the cytoplasm during skeletal muscle ischemia, and was released into the systemic circulation after reperfusion in mice with I/R injury. Treatment with anti-HMGB1 antibodies partially improved survival.

The preventive role of medical ozone therapy on remote organ injury induced by hepatic ischemia reperfusion

2021 ◽  
Vol 28 (9) ◽  
pp. 1671
Author(s):  
Levent Demirtas ◽  
Cebrail Gursul ◽  
Ahmet Gurbuzel ◽  
Ilyas Sayar ◽  
Mehmet Gurbuzel ◽  
...  
Keyword(s):  
Ischemia Reperfusion ◽  
Organ Injury ◽  
Ozone Therapy ◽  
Hepatic Ischemia ◽  
Remote Organ ◽  
Remote Organ Injury ◽  
Medical Ozone ◽  
Hepatic Ischemia Reperfusion ◽  
Preventive Role

scholarly journals Orexigenic Hormone Ghrelin Attenuates Local and Remote Organ Injury after Intestinal Ischemia-Reperfusion

PLoS ONE ◽  
2008 ◽  
Vol 3 (4) ◽  
pp. e2026 ◽  
Author(s):  
Rongqian Wu ◽  
Weifeng Dong ◽  
Youxin Ji ◽  
Mian Zhou ◽  
Corrado P. Marini ◽  
...  
Keyword(s):  
Intestinal Ischemia ◽  
Ischemia Reperfusion ◽  
Organ Injury ◽  
Remote Organ ◽  
Remote Organ Injury ◽  
Intestinal Ischemia Reperfusion

LOW VOLUME HYPERTONIC SALINE ABROGATES MESENTERIC ISCHEMIA/REPERFUSION INDUCED REMOTE ORGAN INJURY

2005 ◽  
Vol 59 (2) ◽  
pp. 527
Author(s):  
Ernest A. Gonzalez ◽  
Rosemary A. Kozar ◽  
James W. Suliburk ◽  
David W. Mercer ◽  
Frederick A. Moore
Keyword(s):  
Hypertonic Saline ◽  
Mesenteric Ischemia ◽  
Ischemia Reperfusion ◽  
Organ Injury ◽  
Remote Organ ◽  
Remote Organ Injury ◽  
Low Volume

scholarly journals A Method to Detect Remote Organ Injury after Intestinal Ischemia-Reperfusion in Mice.

2002 ◽  
Vol 13 (3) ◽  
pp. 144-150
Author(s):  
Yuriko Matsuura ◽  
Kaoru Koike ◽  
Atsuko Tsujii ◽  
Saeed Samarghandian ◽  
Shigeki Kushimoto ◽  
...  
Keyword(s):  
Intestinal Ischemia ◽  
Ischemia Reperfusion ◽  
Organ Injury ◽  
Remote Organ ◽  
Remote Organ Injury ◽  
Intestinal Ischemia Reperfusion

scholarly journals Protective effect of N-acetylcysteine on kidney as a remote organ after skeletal muscle ischemia-reperfusion

2012 ◽  
Vol 27 (9) ◽  
pp. 611-615 ◽  
Author(s):  
Mohammad Ashrafzadeh Takhtfooladi ◽  
Amirali Jahanshahi ◽  
Gholamreza Jahanshahi ◽  
Amir Sotoudeh ◽  
Hamed Ashrafzadeh Takhtfooladi ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Protective Effect ◽  
Femoral Artery ◽  
Renal Injury ◽  
Ischemia Reperfusion ◽  
Male Rats ◽  
Muscle Ischemia ◽  
Group I ◽  
Remote Organ ◽  
Group Ii

PURPOSE: To investigate whether N-acetylcysteine has a protective effect against renal injury as a remote organ after skeletal muscle ischemia-reperfusion in rats. METHODS: Twenty Wistar male rats were divided randomly into two experimental groups: group ischemia-reperfusion (group I) and group ischemia-reperfusion + N-acetylcysteine (group II). After ketamine and xylazine anesthesia, femoral artery was exposed. All animals were undergone 2h of ischemia by occlusion femoral artery and 24h of reperfusion. Rats that were treated with N-acetylcysteine given IV at a dose of 150 mg/kg-¹, immediately before reperfusion. After 24h of reperfusion, the blood samples were collected and submitted for evaluation of plasmatic urea, creatinine values and then rats were euthanized and left kidney harvested for histopathological analysis under light microscopy. RESULTS: The urea (35±7.84 mg.dL-1), creatinine (1.46±0.47 mg.dL-1) values were significantly lower in group II (P=0.000). Renal histopathologic study in group I showed extensive distal and proximal tubular cells necrosis and sloughing of epithelial cells into the tubular lumen, cast formation in tubule and glomerul, glomerul fibrosis and hemorrhage. Histopathologically, there was a significant difference (p=0.037) between two groups. CONCLUSION: The N-acetylcysteine was able to decrease renal injury induced by skeletal muscle ischemia reperfusion in rats.

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