scholarly journals miR-10c Facilitates White Spot Syndrome Virus Infection by Targeting Toll3 in Litopenaeus vannemei

2021 ◽  
Vol 12 ◽  
Author(s):  
Hongliang Zuo ◽  
Xinxin Liu ◽  
Mengting Luo ◽  
Linwei Yang ◽  
Zhiming Zhu ◽  
...  

Toll-like receptors (TLRs) are canonical cell membrane receptors functioning to recognize pathogens and transduce signals to activate immune responses. It has been known that Toll3 in Pacific white shrimp Litopenaeus vannamei (LvToll3) plays a critical role in antiviral immunity by inducing the transcription of interferon regulatory factor (IRF), which mediates a signaling axis that is similar to the interferon system of vertebrates. However, the regulatory mechanism of the Toll3-IRF signaling is still unclear. In this study, a novel microRNA (miRNA) of miR-10 family, temporarily named as miR-10c, was identified from L. vannamei. miR-10c may play a nonnegligible regulatory role in shrimp immune responses since it was constitutively expressed in all detected tissues and transcriptionally induced by immune stimulation. Functional analysis validated that miR-10c could target LvToll3 to inhibit its expression, through which miR-10c blocked the nuclear translocation of IRF and facilitated white spot syndrome virus (WSSV) infection. To our knowledge, the present study revealed the first report of a Toll targeted by miRNA in crustaceans and provided a solid evidence base for supporting the role of LvToll3 in antiviral defense by activating IRF signaling in L. vannamei. Identification of the miR-10c/Toll3/IRF regulatory axis in shrimp provides new insights into the participation of miRNA in the regulation of immune responses and contributes to in-depth understanding of the mechanisms of Toll-induced immune responses in L. vannamei.

Aquaculture ◽  
2015 ◽  
Vol 447 ◽  
pp. 102-107 ◽  
Author(s):  
Alejandra Caballero-Zamora ◽  
Hugo H. Montaldo ◽  
Gabriel Ricardo Campos-Montes ◽  
Eugenia Guadalupe Cienfuegos-Rivas ◽  
Alfonso Martínez-Ortega ◽  
...  

2013 ◽  
Vol 36 (12) ◽  
pp. 977-985 ◽  
Author(s):  
L Cheng ◽  
W-H Lin ◽  
P-C Wang ◽  
M-A Tsai ◽  
J-P Hsu ◽  
...  

2019 ◽  
Vol 28 (1) ◽  
pp. 293-307 ◽  
Author(s):  
Cecília de Souza Valente ◽  
Karla Oliveira Ortiz ◽  
Rachel Depperschmidt ◽  
Ana Paula de Medeiros Fraga ◽  
Felipe do Nascimento Vieira ◽  
...  

Aquaculture ◽  
2015 ◽  
Vol 435 ◽  
pp. 306-309 ◽  
Author(s):  
Dharnappa Sannejal Akhila ◽  
Madhu K. Mani ◽  
Praveen Rai ◽  
Kelly Condon ◽  
Leigh Owens ◽  
...  

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